{"search_session":{},"preferences":{"l":"fr","queryLanguage":"fr"},"patentId":"099-666-460-399-191","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"099-666-460-399-191","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10731,"type":"PATENT","title":"The University of North Carolina at Chapel Hill - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":8887,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8232,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately:\"uni* north caro*\". Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1000
Search Applicants and Owners separately:\"uni* north caro*\". Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1000
(a) providing a subject under consideration for a therapy;\n
(b) providing a sample from the subject, wherein the sample comprises a cell or tissue having a molecular age;\n
(c) determining the molecular age of the cell or tissue by:\n\n(i) quantifying a level of expression of p16INK4a, ARF, or both p16INK4a and ARF in the sample;\n(ii) comparing the level of expression quantified with a standard, wherein the comparing comprises determining whether an increased level of expression of p16INK4a, ARF, or both p16INK4a and ARF is present or absent in the sample relative to the standard; and\n(iii) determining an increased molecular age if an increased level is present or a lack of an increased molecular age if an increased level is absent; and\n
(d) predicting an increased tendency to therapy-induced toxicity in the subject if an increased molecular age is present or a decreased tendency to therapy-induced toxicity in the subject if an increased molecular age is absent."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, further comprising treating the subject with the therapy if a decreased tendency to therapy-induced toxicity is predicted or not treating the subject with the therapy if an increased tendency to therapy-induced toxicity is predicted."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, further comprising treating the subject with the therapy and one or more additional supportive treatments if an increased tendency to therapy-induced toxicity is predicted."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["A method for determining suitability of a cell or tissue for use in a donation, the method comprising:\n
(a) providing a sample from the cell or tissue, wherein the cell or tissue has a molecular age;\n
(b) determining the molecular age of the cell or tissue by:\n\n(i) quantifying a level of expression of p16INK4a, ARF, or both p16INK4a and ARF in the sample;\n(ii) comparing the level of expression quantified with a standard, wherein the comparing comprises determining whether an increased level of expression of p16INK4a, ARF, or both p16INK4a and ARF is present or absent in the sample relative to the standard; and\n(iii) determining the molecular age based on whether an increased level is present or absent; and\n
(c) determining a suitability of the cell or tissue for use in a donation based on the molecular age."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 2, wherein the cell or tissue donation is appropriate for a transplant selected from the group consisting of bone marrow transplant, solid organ transplant, and tissue graft transplant."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["A method for determining the molecular age of a cell or tissue in a subject, the method comprising:\n
a) providing a sample from the cell or tissue;\n
b) quantifying a level of expression of p16INK4a, ARF, or both p16INK4a and ARF in the sample; and\n
c) determining the molecular age of the cell or tissue by comparing the level of expression of p16INK4a, ARF, or both p16INK4a and ARF to a standard."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claims 1, 4, or 6, wherein the sample comprises a cell or tissue selected from the group consisting of an adrenal gland cell, a bone marrow cell, a cecum cell, a brain cortex cell, a duodenum cell, a heart cell, a kidney cell, a liver cell, a lung cell, a lymph node cell, an ovary cell, a pancreas cell, a spleen cell, a testis cell, a blood cell, and a uterus cell."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claims 1, 4, or 6, further comprising purifying a particular cell type from tissue."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 5, wherein the particular cell type comprises a lymphocyte, a stem cell, a neural cell, and an islet cell."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claims 1, 4 or 6, wherein the standard comprises a quantification of a level of expression of p16INK4a, ARF, or both p16INK4a and ARF in a cell or tissue in a population of same species individuals as the subject."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claims 1, 4, or 6, wherein the standard comprises a quantification of a level of expression of p16INK4a, ARF, or both p16INK4a and ARF in a cell or tissue in the subject at an earlier time."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method of claims 1, 4, or 6, wherein the standard comprises a quantification of a level of expression of p16INK4a, ARF, or both p16INK4a and ARF in a second sample isolated from a different tissue of the subject."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9, wherein the at least one different tissue comprises a cell selected from the group consisting of an adrenal gland cell, a bone marrow cell, a cecum cell, a brain cortex cell, a duodenum cell, a heart cell, a kidney cell, a liver cell, a lung cell, a lymph node cell, an ovary cell, a pancreas cell, a spleen cell, a testis cell, a blood cell, and a uterus cell."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method of claims 1, 4, or 6, wherein the subject is a human."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claims 1, 4, or 6, wherein the quantifying employs quantitative real-time polymerase chain reaction analysis."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claims 1, 4, or 6, wherein the comparing the level comprises determining an increased level of expression of p16INK4a, ARF, or both p16INK4a and ARF, and the increased level of expression indicates increased molecular age of the tissue relative to the standard."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method of claims 1, 4, or 6, wherein the comparing is to a level of expression of p16INK4a, ARF, or both p16INK4a and ARF in the target cell type in a population of same species individuals as the subject."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method of claims 1, 4, or 6, further comprising normalizing the level of expression quantified to a level of expression of a housekeeping gene."],"number":18,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}