US 7674291 B2 - Human Skin Equivalents Expressing Exogenous Polypeptides - The Lens

Human Skin Equivalents Expressing Exogenous Polypeptides

Published: Mar 9, 2010
Earliest Priority: Aug 01 2003
Family: 35
Cited Works: 50
Cited by: 13
Cites: 20
Sequences: 124
Additional Info: Cited Works Full text Published Sequence

Data obtained from: NCBI GenBank EMBL-EBI Pat USPTO Fulltext DDBJ Pat

124 sequences found in this patent (in total)

Download sequences in fasta format: 105 peptides 19 DNAs

Displaying 1 - 10 of 124 sequences

SEQ ID NO

Length

Sequence Type

Locations

Declared organism

Determined organism

Occurrence

Tools

SEQ ID NO

Length

Sequence Type

DNA

Locations

Detailed Description

Declared organism

Artificial

Determined organism

Occurrence

21 times in PatSeqDB

in 2 simple families
in 21 documents

Tools

SEQ ID NO

Length

Sequence Type

DNA

Locations

Detailed Description

Declared organism

Artificial

Determined organism

Occurrence

21 times in PatSeqDB

in 2 simple families
in 21 documents

Tools

SEQ ID NO

Length

1,767

Sequence Type

DNA

Locations

Detailed Description

Declared organism

Artificial

Determined organism

Occurrence

21 times in PatSeqDB

in 2 simple families
in 21 documents

Tools

SEQ ID NO

Length

Sequence Type

DNA

Locations

Detailed Description

Declared organism

Artificial

Determined organism

Occurrence

21 times in PatSeqDB

in 2 simple families
in 21 documents

Tools

SEQ ID NO

Length

Sequence Type

DNA

Locations

Detailed Description

Declared organism

Artificial

Determined organism

Occurrence

21 times in PatSeqDB

in 2 simple families
in 21 documents

Tools

SEQ ID NO

Length

Sequence Type

DNA

Locations

Detailed Description

Declared organism

Artificial

Determined organism

Homo sapiens
Homo sapiens
Public DB hit
GI:224809241
NG_011446
1 of 10 hits reported for Homo sapiens.

Hit length
90,688 bp

E Value
2.55E-2

BLAST Score
42.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 6

100% cov
NG_011446

0.02% cov

Alignment Length
21 bp

CIGAR string
21M mismatches: 0 - insertions: 0 - deletions: 0
Pan troglodytes
Pan troglodytes
Public DB hit
GI:332821476
XM_526931
1 of 2 hits reported for Pan troglodytes.

Hit length
2,461 bp

E Value
2.55E-2

BLAST Score
42.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 6

100% cov
XM_526931

0.85% cov

Alignment Length
21 bp

CIGAR string
21M mismatches: 0 - insertions: 0 - deletions: 0
Nomascus leucogenys
Nomascus leucogenys
Public DB hit
GI:332257427
XM_003277758
1 of 2 hits reported for Nomascus leucogenys.

Hit length
2,471 bp

E Value
2.55E-2

BLAST Score
42.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 6

100% cov
XM_003277758

0.85% cov

Alignment Length
21 bp

CIGAR string
21M mismatches: 0 - insertions: 0 - deletions: 0
Bos taurus
Bos taurus
Public DB hit
GI:329744554
NM_001206326
1 of 2 hits reported for Bos taurus.

Hit length
3,384 bp

E Value
2.55E-2

BLAST Score
42.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 6

100% cov
NM_001206326

0.62% cov

Alignment Length
21 bp
Sus scrofa
Sus scrofa
Public DB hit
GI:311273663
XM_003133924
1 of 2 hits reported for Sus scrofa.

Hit length
1,426 bp

E Value
2.55E-2

BLAST Score
42.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 6

100% cov
XM_003133924

1.47% cov

Alignment Length
21 bp
Pongo abelii
Pongo abelii
Public DB hit
GI:297675220
XM_002815541
1 of 2 hits reported for Pongo abelii.

Hit length
3,665 bp

E Value
2.55E-2

BLAST Score
42.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 6

100% cov
XM_002815541

0.57% cov

Alignment Length
21 bp
Oryctolagus cuniculus
Oryctolagus cuniculus
Public DB hit
GI:291395320
XM_002714023
1 of 2 hits reported for Oryctolagus cuniculus.

Hit length
663 bp

E Value
2.55E-2

BLAST Score
42.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 6

100% cov
XM_002714023

3.17% cov

Alignment Length
21 bp
Equus caballus
Equus caballus
Public DB hit
GI:149732756
XM_001498109
1 of 2 hits reported for Equus caballus.

Hit length
651 bp

E Value
2.55E-2

BLAST Score
42.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 6

100% cov
XM_001498109

3.23% cov

Alignment Length
21 bp
synthetic construct
synthetic construct
Public DB hit
GI:313882557
HQ258011

Hit length
658 bp

E Value
2.55E-2

BLAST Score
42.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 6

100% cov
HQ258011

3.19% cov

Alignment Length
21 bp

Occurrence

21 times in PatSeqDB

in 2 simple families
in 21 documents

Tools

SEQ ID NO

Length

Sequence Type

DNA

Locations

Detailed Description

Declared organism

Artificial

Determined organism

Equus caballus
Equus caballus
Public DB hit
GI:255683514
NM_001164018
1 of 2 hits reported for Equus caballus.

Hit length
444 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
NM_001164018

4.5% cov

Alignment Length
20 bp

CIGAR string
20M mismatches: 0 - insertions: 0 - deletions: 0
PiggyBac insertional mutagenesis vector Slingshot-PB
PiggyBac insertional mutagenesis vector Slingshot-PB
Public DB hit
GI:294985311
GU937109

Hit length
15,199 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
GU937109

0.13% cov

Alignment Length
20 bp

CIGAR string
20M mismatches: 0 - insertions: 0 - deletions: 0
Cloning vector pMAZe
Cloning vector pMAZe
Public DB hit
GI:288188775
GQ856364

Hit length
11,860 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
GQ856364

0.17% cov

Alignment Length
20 bp

CIGAR string
20M mismatches: 0 - insertions: 0 - deletions: 0
synthetic construct
synthetic construct
Public DB hit
GI:197205403
EU919427

Hit length
4,794 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
EU919427

0.42% cov

Alignment Length
20 bp
Mammalian expression vector pNBioSec
Mammalian expression vector pNBioSec
Public DB hit
GI:158534336
EU082004

Hit length
7,032 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
EU082004

0.28% cov

Alignment Length
20 bp
Expression vector pP0-Cre
Expression vector pP0-Cre
Public DB hit
GI:158302652
AB363405

Hit length
6,350 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
AB363405

0.31% cov

Alignment Length
20 bp
Cloning vector pU.CAG.Hand1
Cloning vector pU.CAG.Hand1
Public DB hit
GI:124361332
EF208956

Hit length
5,947 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
EF208956

0.34% cov

Alignment Length
20 bp
Cloning vector pU.CAG.MyocS
Cloning vector pU.CAG.MyocS
Public DB hit
GI:124361330
EF208955

Hit length
7,910 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
EF208955

0.25% cov

Alignment Length
20 bp
Shuttle vector pUCAG.TBX5
Shuttle vector pUCAG.TBX5
Public DB hit
GI:122893047
EF186088

Hit length
6,630 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
EF186088

0.3% cov

Alignment Length
20 bp
Shuttle vector pUCAG.MEF2C
Shuttle vector pUCAG.MEF2C
Public DB hit
GI:122893045
EF186087

Hit length
6,382 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
EF186087

0.31% cov

Alignment Length
20 bp
Shuttle vector pUCAG.GATA4
Shuttle vector pUCAG.GATA4
Public DB hit
GI:122893043
EF186086

Hit length
6,388 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
EF186086

0.31% cov

Alignment Length
20 bp
Shuttle vector pUCAG.nkx2.5
Shuttle vector pUCAG.nkx2.5
Public DB hit
GI:122893041
EF186085

Hit length
6,175 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
EF186085

0.32% cov

Alignment Length
20 bp
Shuttle vector pUCAG.myocardin
Shuttle vector pUCAG.myocardin
Public DB hit
GI:122893039
EF186084

Hit length
8,054 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
EF186084

0.25% cov

Alignment Length
20 bp
Shuttle vector pUCAG.Gfp
Shuttle vector pUCAG.Gfp
Public DB hit
GI:122893037
EF186083

Hit length
5,761 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
EF186083

0.35% cov

Alignment Length
20 bp
Expression vector pCXGFP-1
Expression vector pCXGFP-1
Public DB hit
GI:117939115
AB281497

Hit length
6,787 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
AB281497

0.29% cov

Alignment Length
20 bp
Ochotona curzoniae
Ochotona curzoniae
Public DB hit
GI:110734519
DQ839484

Hit length
444 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
DQ839484

4.5% cov

Alignment Length
20 bp
Transposon vector T2AR200R200G
Transposon vector T2AR200R200G
Public DB hit
GI:110082649
AB262456

Hit length
2,602 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
AB262456

0.77% cov

Alignment Length
20 bp
Transposon vector T2AL200L200G
Transposon vector T2AL200L200G
Public DB hit
GI:110082647
AB262455

Hit length
2,602 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
AB262455

0.77% cov

Alignment Length
20 bp
Transposon vector T2AL50R50G
Transposon vector T2AL50R50G
Public DB hit
GI:110082645
AB262454

Hit length
2,302 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
AB262454

0.87% cov

Alignment Length
20 bp
Transposon vector T2AL200R100G
Transposon vector T2AL200R100G
Public DB hit
GI:110082643
AB262453

Hit length
2,502 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
AB262453

0.8% cov

Alignment Length
20 bp
Transposon vector T2AL200R150G
Transposon vector T2AL200R150G
Public DB hit
GI:110082641
AB262452

Hit length
2,552 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
AB262452

0.78% cov

Alignment Length
20 bp
Transposon vector T2AL200R175G
Transposon vector T2AL200R175G
Public DB hit
GI:110082639
AB262451

Hit length
2,577 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
AB262451

0.78% cov

Alignment Length
20 bp
Transposon vector T2AL150R200G
Transposon vector T2AL150R200G
Public DB hit
GI:110082637
AB262450

Hit length
2,552 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
AB262450

0.78% cov

Alignment Length
20 bp
Transposon vector T2AL175R200G
Transposon vector T2AL175R200G
Public DB hit
GI:110082635
AB262449

Hit length
2,577 bp

E Value
6.71E-2

BLAST Score
40.1 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 7

100% cov
AB262449

0.78% cov

Alignment Length
20 bp

Occurrence

21 times in PatSeqDB

in 2 simple families
in 21 documents

Tools

SEQ ID NO

Length

Sequence Type

DNA

Locations

Detailed Description

Declared organism

Artificial

Determined organism

Schistosoma mansoni
Schistosoma mansoni
Public DB hit
GI:238667843
FN363668

Hit length
1,631 bp

E Value
2.65E-7

BLAST Score
61.9 bits

Similarity

100%

Coverage

62%

Alignment (schematic)

SEQ ID NO 8

62% cov
FN363668

1.9% cov

Alignment Length
31 bp

CIGAR string
19H31M mismatches: 0 - insertions: 0 - deletions: 0
Danio rerio
Danio rerio
Public DB hit
GI:152012990
BC150167
1 of 3 hits reported for Danio rerio.

Hit length
4,415 bp

E Value
2.65E-7

BLAST Score
61.9 bits

Similarity

100%

Coverage

62%

Alignment (schematic)

SEQ ID NO 8

62% cov
BC150167

0.7% cov

Alignment Length
31 bp

CIGAR string
19H31M mismatches: 0 - insertions: 0 - deletions: 0
Mus musculus
Mus musculus
Public DB hit
GI:20988791
BC030176
1 of 6 hits reported for Mus musculus.

Hit length
847 bp

E Value
2.65E-7

BLAST Score
61.9 bits

Similarity

100%

Coverage

62%

Alignment (schematic)

SEQ ID NO 8

62% cov
BC030176

3.66% cov

Alignment Length
31 bp
Xenopus laevis
Xenopus laevis
Public DB hit
GI:92447194
BC072757
1 of 4 hits reported for Xenopus laevis.

Hit length
4,994 bp

E Value
2.65E-7

BLAST Score
61.9 bits

Similarity

100%

Coverage

62%

Alignment (schematic)

SEQ ID NO 8

62% cov
BC072757

0.62% cov

Alignment Length
31 bp
Aspergillus oryzae
Aspergillus oryzae
Public DB hit
GI:84577790
AB229964
1 of 7 hits reported for Aspergillus oryzae.

Hit length
585 bp

E Value
2.65E-7

BLAST Score
61.9 bits

Similarity

100%

Coverage

62%

Alignment (schematic)

SEQ ID NO 8

62% cov
AB229964

5.3% cov

Alignment Length
31 bp
Homo sapiens
Homo sapiens
Public DB hit
GI:18042967
BC020376
1 of 3 hits reported for Homo sapiens.

Hit length
1,569 bp

E Value
2.65E-7

BLAST Score
61.9 bits

Similarity

100%

Coverage

62%

Alignment (schematic)

SEQ ID NO 8

62% cov
BC020376

1.98% cov

Alignment Length
31 bp
Cucumis sativus
Cucumis sativus
Public DB hit
GI:2462264
Y12793

Hit length
1,297 bp

E Value
2.65E-7

BLAST Score
61.9 bits

Similarity

100%

Coverage

62%

Alignment (schematic)

SEQ ID NO 8

62% cov
Y12793

2.39% cov

Alignment Length
31 bp

Occurrence

21 times in PatSeqDB

in 2 simple families
in 21 documents

Tools

SEQ ID NO

Length

322

Sequence Type

DNA

Locations

Detailed Description, Drawings

Declared organism

Homo sapiens

Determined organism

Homo sapiens
Homo sapiens
Public DB hit
GI:1755147
U73945
1 of 5 hits reported for Homo sapiens.

Hit length
322 bp

E Value
5.41E-167

BLAST Score
595.7 bits

Similarity

100%

Coverage

100%

Alignment (schematic)

SEQ ID NO 9

100% cov
U73945

100% cov

Alignment Length
322 bp

CIGAR string
322M mismatches: 0 - insertions: 0 - deletions: 0
Nomascus leucogenys
Nomascus leucogenys
Public DB hit
GI:332244598
XM_003271413
1 of 2 hits reported for Nomascus leucogenys.

Hit length
463 bp

E Value
2.61E-140

BLAST Score
507.1 bits

Similarity

95.05%

Coverage

100%

Alignment (schematic)

SEQ ID NO 9

100% cov
XM_003271413

69.55% cov

Alignment Length
323 bp

CIGAR string
28MN22MN83MN15MN17MN8MN8MN32MN4MN25MIMD3MN42MN2MN7MN4MN6M mismatches: 14 - insertions: 1 - deletions: 1
Pongo abelii
Pongo abelii
Public DB hit
GI:297682218
XM_002818778
1 of 2 hits reported for Pongo abelii.

Hit length
478 bp

E Value
2.61E-140

BLAST Score
507.1 bits

Similarity

95.31%

Coverage

99.07%

Alignment (schematic)

SEQ ID NO 9

99.07% cov
XM_002818778

66.74% cov

Alignment Length
320 bp

CIGAR string
28MN22MN34MN64MN17MN17MN23MN8MIMD12MN17MN37M2N10MN3MN12M3H mismatches: 13 - insertions: 1 - deletions: 1
Pongo pygmaeus
Pongo pygmaeus
Public DB hit
GI:134802575
AM410099

Hit length
228 bp

E Value
9.71E-115

BLAST Score
422.2 bits

Similarity

100%

Coverage

70.81%

Alignment (schematic)

SEQ ID NO 9

70.81% cov
AM410099

100% cov

Alignment Length
228 bp

CIGAR string
71H228M23H mismatches: 0 - insertions: 0 - deletions: 0
Pan troglodytes
Pan troglodytes
Public DB hit
GI:134802566
AM410097
1 of 3 hits reported for Pan troglodytes.

Hit length
228 bp

E Value
2.1E-111

BLAST Score
411.1 bits

Similarity

99.12%

Coverage

70.81%

Alignment (schematic)

SEQ ID NO 9

70.81% cov
AM410097

100% cov

Alignment Length
228 bp

CIGAR string
71H107MN72MN47M23H mismatches: 2 - insertions: 0 - deletions: 0
Gorilla gorilla
Gorilla gorilla
Public DB hit
GI:134802571
AM410098

Hit length
228 bp

E Value
4.55E-108

BLAST Score
400 bits

Similarity

98.25%

Coverage

70.81%

Alignment (schematic)

SEQ ID NO 9

70.81% cov
AM410098

100% cov

Alignment Length
228 bp

CIGAR string
71H53MN26MN59MN39MN47M23H mismatches: 4 - insertions: 0 - deletions: 0
synthetic construct
synthetic construct
Public DB hit
GI:312153013
HQ448540
1 of 2 hits reported for synthetic construct.

Hit length
306 bp

E Value
4.58E-103

BLAST Score
383.4 bits

Similarity

100%

Coverage

64.29%

Alignment (schematic)

SEQ ID NO 9

64.29% cov
HQ448540

67.65% cov

Alignment Length
207 bp

CIGAR string
70H207M45H mismatches: 0 - insertions: 0 - deletions: 0
Hylobates lar
Hylobates lar
Public DB hit
GI:134802577
AM410100

Hit length
228 bp

E Value
2.15E-96

BLAST Score
361.2 bits

Similarity

95.2%

Coverage

70.81%

Alignment (schematic)

SEQ ID NO 9

70.81% cov
AM410100

100% cov

Alignment Length
229 bp

CIGAR string
71H64MN15MN17MN8MN8MN23MN8MN4MN25MIMD3MN42M23H mismatches: 9 - insertions: 1 - deletions: 1

Occurrence

36 times in PatSeqDB

in 6 extended families
(1 referenced in claims)
in 8 simple families
(1 referenced in claims)
in 36 documents

Tools

SEQ ID NO

Length

319

Sequence Type

DNA

Locations

Detailed Description, Drawings

Declared organism

Homo sapiens

Determined organism

Occurrence

29 times in PatSeqDB

in 3 extended families
(1 referenced in claims)
in 5 simple families
(2 referenced in claims)
in 29 documents

Tools

124 sequences found in this patent (in total)

Filter sequences

Displaying 1 - 10 of 124 sequences

Cloning vector 3'HPRT

Public DB hit

Hit length

E Value

BLAST Score

Similarity

Coverage

Alignment (schematic)

Alignment Length

CIGAR string

Homo sapiens

Public DB hit

Hit length

E Value

BLAST Score

Similarity

Coverage

Alignment (schematic)

Alignment Length

CIGAR string

Homo sapiens

Public DB hit

Hit length

E Value

BLAST Score

Similarity

Coverage

Alignment (schematic)

Alignment Length

CIGAR string

Pan troglodytes

Public DB hit

Hit length

E Value

BLAST Score

Similarity

Coverage

Alignment (schematic)

Alignment Length

CIGAR string

Nomascus leucogenys

Public DB hit

Hit length

E Value

BLAST Score

Similarity

Coverage

Alignment (schematic)

Alignment Length

Homo sapiens

Public DB hit

Hit length

E Value

BLAST Score

Similarity

Coverage

Alignment (schematic)

Alignment Length

CIGAR string

Cloning vector 3'HPRT

Public DB hit

Hit length

E Value

BLAST Score

Similarity

Coverage

Alignment (schematic)

Alignment Length

Expression vector pcDNA3-hFIX

Public DB hit

Hit length

E Value

BLAST Score

Similarity

Coverage

Alignment (schematic)

Alignment Length