Lipid-encapsulated Polyanionic Nucleic Acid


Methods for the preparation of a lipid-nucleic acid composition are provided. According to the methods, a mixture of lipids containing a protonatable or deprotonatable lipid, for example an amino lipid and a lipid such as a PEG- or Polyamide oligomer-modified lipid is combined with a buffered aqueous solution of a charged therapeutic agent, for example polyanionic nucleic acids, to produce particles in which the therapeutic agent is encapsulated in a lipid vesicle. Surface charges on the lipid particles are at least partially neutralized to provide surface-neutralized lipid-encapsulated compositions of the therapeutic agents. The method permits the preparation of compositions with high ratios of therapeutic agent to lipid and with encapsulation efficiencies in excess of 50%.

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Document History
  • Publication: Sep 20, 2011
  • Application: Feb 8, 2008
    US US 2869608 A
  • Priority: Feb 8, 2008
    US US 2869608 A
  • Priority: Sep 9, 2003
    US US 65894703 A
  • Priority: Jun 29, 2001
    US US 89548001 A
  • Priority: May 14, 1998
    US US 7895498 A
  • Priority: May 14, 1997
    US US 85637497 A

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