{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"074-346-231-907-594","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"074-346-231-907-594"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8759,"type":"PATENT","title":"University of Columbia","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":13487,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8203,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Searched applicants and Owners= \"Columbia Univ\", \"Univ Columbia\", \" Univ Colum*\", \"Colum* univ\", \"Univ* Colum* NOT British\".
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Searched applicants and Owners= \"Columbia Univ\", \"Univ Columbia\", \" Univ Colum*\", \"Colum* univ\", \"Univ* Colum* NOT British\".
Select more for logical Variants
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Total patents: more than 10k
(a) obtaining a sample from the subject;\n
(b) quantitatively determining the amount of an early pregnancy associated molecular isoform of human chorionic gonadotropin (hCG) which isoform is recognized by the antibody produced by hybridoma cell line B152 (ATCC Designation No. HB-12467) in the sample;\n
(c) comparing the amount of the early pregnancy associated molecular isoform of hCG in the sample determined in step (b) with a reference amount determined for samples from either temporally matched, normal pregnant subjects or non-pregnant subjects; and\n
(d) administering to the subject an effective amount of the early pregnancy associated molecular isoform of hCG to reduce the risk of pregnancy loss, if the amount of the early pregnancy associated molecular isoform of hCG in the sample from the subject is less than the reference amount present in the samples from the temporally matched, normal pregnant subjects or from non-pregnant subjects, so as to thereby reduce the risk of pregnancy loss in the subject."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the sample is a urine sample or a blood sample."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein step (b) comprises:\n
(i) contacting the sample with an amount of a capture antibody which specifically binds to the early pregnancy associated molecular isoform of hCG, under conditions permitting formation of a complex between the capture antibody and the early pregnancy associated molecular isoform of hCG; and\n
(ii) measuring the amount of complexes formed in step (i), thereby determining the amount of the early pregnancy associated molecular isoform of hCG in the sample."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, wherein the capture antibody is B152."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein step (b) comprises:\n
(i) contacting the sample with an amount of a capture antibody which specifically binds to the early pregnancy associated molecular isoform of hCG, under conditions permitting formation of a complex between the capture antibody and the early pregnancy associated molecular isoform of hCG;\n
(ii) contacting the complexes formed in step (i) with an amount of a detection antibody under conditions permitting binding of the detection antibody to the complexes; and\n
(iii)measuring the amount of detection antibodies bound to the complexes so as to thereby determine the amount of the early pregnancy associated molecular isoform of hCG in the sample."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 5, wherein the capture antibody is B152."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["the method of claim 5, wherein the detection antibody is B207."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["the method of claim 7, wherein the detection antibody is labeled with a detectable marker."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 8, wherein the detectable marker is a radioactive isotope, enzyme, dye, magnetic bead, or biotin."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 9, wherein the radioactive isotope is I125."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein step (b) comprises:\n
(i) contacting an amount of a capture antibody which specifically binds to the early pregnancy associated molecular isoform of hCG with a solid matrix under conditions permitting binding of the capture antibody with the solid matrix;\n
(ii) contacting the sample with the solid matrix under conditions permitting binding of the early pregnancy associated molecular isoform of hCG present in the sample with the capture antibody bound to the solid matrix;\n
(iii) separating the solid matrix and the sample;\n
(iv) contacting the separated solid matrix with a detection antibody; and\n
(v) measuring the amount of the detection antibody bound on the solid matrix, thereby determining the amount of early pregnancy associated molecular isoform of hCG in the sample."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 11, wherein the capture antibody is B152."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["the method of claim 12, wherein the detection antibody is labeled with a detectable marker."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 13, wherein the detectable marker is a radioactive isotope, enzyme, dye, magnetic bead, or biotin."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 14, wherein the radioactive isotope is I125."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["the method of claim 11, wherein the detection antibody is B207."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["A method for reducing the risk of pregnancy loss in a subject which comprises:\n
(a) obtaining a sample from the subject;\n
(b) quantitatively determining the amount of an early pregnancy associated molecular isoform of hCG which isoform is recognized by the antibody produced by hybridoma cell line B152 (ATCC Designation No. HB-12467) in the sample;\n
(c) quantitatively determining the amount of intact non-nicked hCC in the sample;\n
(d) determining the ratio of the amount of the early pregnancy associated molecular isoform of hCG in the sample to the amount of intact non-nicked hCG in the sample;\n
(e) comparing the ratio determined in step (d) with a reference ratio determined for samples from either temporally matched, normal pregnant subjects or non-pregnant subjects; and\n
(f) administering to the subject an effective amount of the early pregnancy associated molecular isoform of hCG to reduce the risk of pregnancy loss if the ratio determined in step (d) is less than the reference ratio determined for the samples from the temporally matched, normal pregnant subjects or from non-pregnant subjects, so as to thereby reduce the risk of pregnancy loss in the subject."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["A method for reducing the risk of pregnancy loss in a subject which comprises:\n
(a) obtaining a sample from the subject for quantitative determination of the amount of an early pregnancy associated molecular isoform of hCG which isoform is recognized by the antibody produced by hybridoma cell line B152 (ATCC Designation No. HB-12467) in the sample, and then for comparison of the amount of the early pregnancy associated molecular isoform of hCG in the sample so determined with a reference amount determined for samples from temporally matched, normal subjects; and\n
(b) administering to the subject an effective amount of the early pregnancy associated molecular isoform of hCG to reduce the risk of pregnancy loss if the amount of the early pregnancy associated molecular isoform of hCG in the sample is less than the reference amount, so as to thereby reduce the risk of pregnancy loss in the subject."],"number":18,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}