Immediate Chromatin Immunoprecipitation And Analysis

Abstract

The present invention relates to a newly developed immediate chromatin immunoprecipitation procedure (“ZipChIP”). ZipChIP significantly reduces the time and increases sensitivity allowing for rapid screening of multiple loci. ZipChIP enables the detection of histone modifications (e.g., H3K4 mono- and trimethylation) and at least two yeast histone demethylases, Jhd2 and Rph1, which were previously found difficult to detect using standard methods. ZipChIP further relates to the enrichment of the histone deacetylase Sir2 at heterochromatin in yeast and enrichment of the chromatin remodeler, PICKLE, in Arabidopsis thaliana.


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Document History
  • Publication: Jun 16, 2016
  • Application: Dec 10, 2015
    US US 201514965732 A
  • Priority: Dec 10, 2015
    US US 201514965732 A
  • Priority: Dec 10, 2014
    US US 201462090087 P

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