{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"060-685-206-814-159","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"060-685-206-814-159"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11813,"type":"PATENT","title":"University of Aberdeen Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":27926,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8443,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: univ* aberdeen. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1658
Search Applicants and Owners separately: univ* aberdeen. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1658
a) introducing the plasmid into said bacteria;\n
b) introducing a mutation in chromosomal gene rho of the bacteria;\n
c) maintaining a functional copy of said chromosomal gene on a conditional replicon in said bacteria;\n
d) culturing said bacteria under a condition permissive for intracellular replication and maintenance of said conditional replicon;\n
e) culturing the bacteria under a restrictive condition that does not permit further intracellular replication of the conditional replicon;\n
f) estimating the level of said plasmid in the bacteria cultured under both permissive and restrictive conditions; and\n
g) comparing the level of said plasmid in the bacteria cultured under permissive and restrictive conditions,\nwherein a difference in the level of plasmid from bacteria cultured under permissive conditions and the level of plasmid from bacteria cultured under restrictive conditions is an indication of increased plasmid content,wherein the mutation in gene rho is a GC-to-TA transition mutation at codon 243 of the gene and the bacteria are E. coli. "],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method as claimed in claim 1, wherein the mutation results in a protein with an alanine-to-glutamate substitution at a corresponding amino acid position."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the plasmid is selected from the group consisting of plasmids bearing the ColE 1 origin of replication and plasmids bearing the p15A origin of replication."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, wherein the plasmids bearing the ColE 1 origin of replication are members of an incompatibility group selected from the group consisting of pBR322, pBR329, pUC plasmids, and pBluescript plasmids bearing the origins of replication of any of these plasmids."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the increase is about 10 fold."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the conditional replicon is pHYD1201."],"number":6,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}