{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"055-722-435-003-279","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"055-722-435-003-279","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8892,"type":"PATENT","title":"University of Wisconsin Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":15082,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8214,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners = \"Wisconsin University\" , \"University of Wisconsin\", \"Wisconsin Univ\", \" Univ Wisconsin\" , \"Wisco* Alum* Res* Found*\".
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Search Applicants and Owners = \"Wisconsin University\" , \"University of Wisconsin\", \"Wisconsin Univ\", \" Univ Wisconsin\" , \"Wisco* Alum* Res* Found*\".
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n and m are 0 or integers equal to 1-3, inclusive, and n+m=0-4, inclusive; the dashed line indicates that a double bond may be present or that the bond may be part of an aromatic group and R4, R6 and R8 are not present if there is a double bond between the carbons or the bond is a part of an aromatic ring;\n
R1 and R2 are groups independently selected from aliphatic, alicyclic, heteroalicyclic, aromatic, or heteroaromatic groups which are optionally substituted, wherein substituents are selected from the group consisting of halides, and OH, OR, COH, COR, COOH, COOR, and N(R′)2 groups where R, independent of other R, is an aliphatic, alicyclic, heteroalicyclic, aromatic or heteroaromatic group and each R′, independent of other R′ are hydrogen, or an aliphatic, alicyclic, heteroalicyclic, aromatic or heteroaromatic groups, R and R′ are optionally substituted with substituents as listed for R1 and R2; in R1 and R2, one or more non-neighboring CH2 groups can be replaced with O, S, CO, COO, or CONR′; and\n
R3-R8, independently, are selected from hydrogens, or aliphatic, alicyclic, heteroalicyclic, aromatic or heteroaromatic groups, which can be optionally substituted as listed for R1 and R2, in R3-R8 one or more non-neighboring CH2 groups can be replaced with O, S, CO, COO, or CONR′ groups, where R′ is defined as for R1 and R2, and where R1 and R2 are optionally covalently linked to form a cyclic group, including a bicyclic group, or two or more of R3-R8 are optionally covalently linked to form a cyclic group, including a bicyclic group."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14 wherein the phosphinothiol has the formula: \n\nwhere:\n
R1 and R2independently, are groups selected from aliphatic, alicyclic, heteroalicyclic, aromatic, or heteroaromatic groups which are optionally substituted, wherein substituents are selected from the group consisting of halides, and OH, OR, COH, COR, COOH, COOR, and N(R′)2 groups, where R, independent of other R, is an aliphatic, alicyclic, heteroalicyclic, aromatic or heteroaromatic group and each R′, independent of other R′, is a hydrogen, or an aliphatic, alicyclic, heteroalicyclic, aromatic or heteroaromatic group, R and R′ are optionally substituted as listed for R1 and R2; and in R1 and R2, one or more non-neighboring CH2 groups can be replaced with O, S, CO, COO, or CONR′; and\n
R3 and R4, independently, are groups selected from hydrogen, or an aliphatic, alicyclic, heteroalicyclic, aromatic or heteroaromatic group, which are optionally substituted as listed for R1 and R2, in R3 and R4 one or more non-neighboring CH2 groups can be replaced with O, S, CO, COO, or CONR′ groups where each R′ is defined as for R1 and R2, and where R1 and R2 are optionally covalently linked to form a cyclic group, including a bicyclic group, or R3 and R4 are optionally covalently linked to form a cyclic group, including a bicyclic group."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 15 wherein in the phosphinothiol, R3 and R4 are both hydrogens and R1 and R2 are selected from a phenyl group or a substituted phenyl group."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 15 wherein the R1 and R2 groups of the phosphinothiol are n-butyl groups."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14 wherein the R1 and R2 groups of the phosphinothiol are polyethers."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 18 wherein at least one of the R1 and R2 groups of the phosphinothiol have the formula: \n\nwhere p is 0 or an integer ranging from 1 to about 10, inclusive, and R is hydrogen or an alkyl group."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14 wherein the phosphinothiol has the formula: \n\nwhere n and m are 0 or 1 and X1-X4 are substituents on the aromatic group, two of which may be covalently linked to form an alicyclic or aromatic ring, where X1-X4 are hydrogens, halides, optionally substituted aliphatic, alicyclic or aromatic groups, OH or OR, COR, COOH or COOR groups, where R is an optionally substituted aliphatic, alicyclic, aromatic, or N(R′)2 group, where each R′, independent of other R′, is a hydrogen, or an optionally substituted aliphatic, alicyclic or aromatic group."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 20 wherein R1 and R2 are phenyl or substituted phenyl groups."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21 wherein R1 and R2 are phenyl groups substituted with electron donating groups or electron withdrawing groups."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["A method for synthesis of a peptide or protein wherein at least one of the amide bonds of the peptide or protein is formed by the method of claim 1."],"number":23,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 23 wherein two peptides formed by conventional solid phase synthesis are ligated by formation of the amide bond."],"number":24,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 23 wherein two peptides or proteins formed by recombinant DNA expression methods are ligated by formation of the amide bond."],"number":25,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 23 wherein a peptide or protein formed by conventional solid phase methods is ligated to a peptide or protein formed by recombinant DNA expression methods by formation of the amide bonds."],"number":26,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 23 wherein a peptide is formed by a sequential series of ligations forming amide bonds between amino acids."],"number":27,"annotation":false,"claim":true,"title":false},{"lines":["A method for labeling a peptide or protein with a reporter molecule which comprises the ligation step of claim 1."],"number":28,"annotation":false,"claim":true,"title":false},{"lines":["A phosphinothiol reagent having the formula: \n\nwhere:\n
n and m are 0 or integers equal to 1-3, inclusive, and n+m=0-4, inclusive; the dashed line indicates that a double bond may be present or that the bond may be part of an aromatic group, and R4, R6 and R8 are not present if there is a double bond between the carbons or the bond is a part of an aromatic ring;\n
R1 and R2 are groups independently selected from aliphatic, alicyclic, heteroalicyclic, aromatic, or heteroaromatic groups which are optionally substituted, wherein substituents are selected from the group consisting of halides, and OH, OR, COH, COR, COOH, COOR, and N(R′)2 groups, where R, independent of other R, is an aliphatic, alicyclic, heteroalicyclic, aromatic or heteroaromatic group and each R′, independent of other R′ is a hydrogen, or an aliphatic, alicyclic, heteroalicyclic, aromatic or heteroaromatic group, R and R′ are optionally substituted as listed above for R1 and R2; and R1 and R2, one or more non-neighboring CH2 groups can be replaced with O, S, GO, COO, or CONR′; and\n
R3-R8, independently, are selected from hydrogens, or aliphatic, alicyclic, heteroalicyclic, aromatic or heteroaromatic groups which are optionally substituted as listed for R1 and R2, in R3-R8 one or more non-neighboring CH2 groups can be replaced with O, S, CO, COO, or CONR′ groups where R′ is defined as for R1 and R2, R1 and R2 are optionally covalently linked to form a cyclic group, including a bicyclic group, and two or more of R3-R8 are optionally covalently linked to form a cyclic group, including a bicyclic group wherein the reagent is not o-(diphenylphosphino)benzenethiol."],"number":29,"annotation":false,"claim":true,"title":false},{"lines":["The reagent of claim 29 wherein the phosphinothiol has the formula: \n\nwhere:\n
R1 and R2, independently, are groups selected from aliphatic, alicyclic, heteroalicyclic, aromatic, or heteroaromatic groups which are optionally substituted, wherein substituents are selected from the group consisting of halides, and OH, OR, COH, COR, COOH, COOR, and N(R′)2 groups, where R, independent of other R, is an aliphatic, alicyclic, heteroalicyclic, aromatic or heteroaromatic group and each R′, independent of other R′ is a hydrogen, or an aliphatic, alicyclic, heteroalicyclic, aromatic or heteroaromatic group, R and R′ are optionally substituted as listed above for R1 and R2; and in R1 and R2, one or more non-neighboring CH2 groups can be replaced with O, S, CO, COO, or CONR′; and\n
R3 and R4, independently, are selected from hydrogens, or aliphatic, alicyclic, heteroalicyclic, aromatic or heteroaromatic groups which are optionally substituted as listed for R1 and R2, in R3 and R4 one or more non-neighboring CH2 groups can be replaced with O, S, CO, COO, or CONR′ groups where R′ is as defined for R1 and R2, and R1 and R2 or R3 and R4 are optionally covalently linked to form a cyclic group, including a bicyclic group."],"number":30,"annotation":false,"claim":true,"title":false},{"lines":["The reagent of claim 30 wherein the R1 and R2 groups of the phosphinothiol are n-butyl groups."],"number":31,"annotation":false,"claim":true,"title":false},{"lines":["The reagent of claim 29 wherein the R1 and R2 groups of the phosphinothiol are polyethers."],"number":32,"annotation":false,"claim":true,"title":false},{"lines":["The reagent of claim 29 wherein at least one of the R1 and R2 groups of the phosphinothiol comprise a moiety having the formula: \n\nwhere p is 0 or an integer ranging from 1 to about 10, inclusive, and R is hydrogen or an alkyl group."],"number":33,"annotation":false,"claim":true,"title":false},{"lines":["The reagent of claim 29 wherein the phosphinothiol has the formula: \n\nwhere n and m are 0 are 1 and X1-X4 are substituents on the aromatic group, two of which may be covalently linked to form an alicyclic or aromatic ring, X1-X4 are hydrogens, halides, optionally substituted aliphatic, alicyclic or aromatic groups, OH or OR, COR, COOH or COOR groups, where R is an optionally substituted aliphatic, alicyclic, aromatic, or N(R′)2 group, where each R′, independent of other R′, is a hydrogen, or an optionally substituted aliphatic, alicyclic or aromatic group."],"number":34,"annotation":false,"claim":true,"title":false},{"lines":["The reagent of claim 34 wherein R1 and R2 are phenyl or substituted phenyl groups."],"number":35,"annotation":false,"claim":true,"title":false},{"lines":["The reagent of claim 34 wherein R1 and R2 are phenyl groups substituted with electron donating groups or electron withdrawing groups."],"number":36,"annotation":false,"claim":true,"title":false},{"lines":["The reagent of claim 34 wherein n and m are both 0."],"number":37,"annotation":false,"claim":true,"title":false},{"lines":["The reagent of claim 34 wherein R1 and R2 are both hydrogens."],"number":38,"annotation":false,"claim":true,"title":false},{"lines":["The reagent of claim 34 where R1 and R2 are both phenyl groups."],"number":39,"annotation":false,"claim":true,"title":false},{"lines":["The reagent of claim 34 where R1 and R2 are both n-butyl groups."],"number":40,"annotation":false,"claim":true,"title":false},{"lines":["The reagent of claim 29 which is (diphenylphosphino)methanethiol."],"number":41,"annotation":false,"claim":true,"title":false},{"lines":["A kit for forming an amide bond between an activated carboxylic acid derivative and an azide which comprises one or more reagents for generating an azide and one or more of the phosphinothiol reagents of claim 29."],"number":42,"annotation":false,"claim":true,"title":false},{"lines":["A kit for synthesis of peptides or proteins which comprises one or more of the phosphinothiol reagents of claim 29 and one or more protective agents for amino acid side chains."],"number":43,"annotation":false,"claim":true,"title":false},{"lines":["The kit of claim 43 further comprising a resin for solid phase synthesis."],"number":44,"annotation":false,"claim":true,"title":false},{"lines":["The kit of claim 44 wherein the resin is a safety-catch resin."],"number":45,"annotation":false,"claim":true,"title":false},{"lines":["The kit of claim 43 further comprising a reagent for generating azido peptides."],"number":46,"annotation":false,"claim":true,"title":false},{"lines":["The kit of claim 43 further comprising a reagent for generating thioesters."],"number":47,"annotation":false,"claim":true,"title":false},{"lines":["A kit of claim 42 further comprising one or more solvents for conducting amide bond formation and optionally containing instructions for carrying out amide bond formation."],"number":48,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the phosphinothioester is a phosphinothioester of a lipid."],"number":49,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 49 wherein the azide is an azido peptide or an azido protein."],"number":50,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the azide is a nucleoside azide."],"number":51,"annotation":false,"claim":true,"title":false},{"lines":["The method or claim 51 wherein the phosphinothioester is a phosphinothioester of a peptide or an azido protein."],"number":52,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the amide bond ligates a saccharide to a peptide."],"number":53,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the amide bond ligates a lipid to a peptide."],"number":54,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the amide bond ligates a nucleoside to a peptide."],"number":55,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the amide bond ligates a nucleic acid to a peptide."],"number":56,"annotation":false,"claim":true,"title":false},{"lines":["The reagent of claim 30 wherein R3 and R4 are both hydrogens."],"number":57,"annotation":false,"claim":true,"title":false},{"lines":["The reagent of claim 57 wherein R1 and R2 are phenyl groups or substituted phenyl groups."],"number":58,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}