{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"053-228-808-388-049","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"053-228-808-388-049","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11624,"type":"PATENT","title":"Mayo Medical School - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":5667,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8299,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: mayo medi*
Select more for logical variants. Add to collection.
Select all patents in the collection and expand by simple families. Add to collection. Total patents: 5671
Search Applicants and Owners separately: mayo medi*
Select more for logical variants. Add to collection.
Select all patents in the collection and expand by simple families. Add to collection. Total patents: 5671
(a) culturing ventral endodermal cells or ventral endodermal-like cells in medium, thereby generating conditioned culture medium, and
(b) harvesting the conditioned culture medium, thereby obtaining a cardiogenic cocktail."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The cardiogenic cocktail of claim 1, wherein said ventral endodermal cells or ventral endodermal-like cells are treated with TNF-α."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The cardiogenic cocktail of claim 1, wherein said TNF-α-treated ventral endodermal cells are from an embryo treated with TNF-α and no human embryos are used."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The cardiogenic cocktail of claims 2 or 3, wherein said TNF-α-treated ventral endodermal-like cells are embryonal carcinomal cells."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The cardiogenic cocktail of any of claims 1-4 comprising TGFβ1, TGFβ2, BMP1, BMP2, BMP5, BMP6, FGF4, FGF5, FGF12, FGF13, FGF15, FGF20, leukemia inhibitory factor, VEGF-C, insulin growth factor-1 and interleukin 6."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The cardiogenic cocktail of any of claims 1-5, further comprising one or more components selected from the group consisting of caspase4, chemokine ligand 1, chemokine ligand 2, chemokine ligand 5, chemokine ligand 7, chemokine ligand 11, chemokine ligand 20, haptoglobin, colony stimulating factor 1, lectin, cholesterol 25-hydroxylase, syntaxin-8, syntaxin-11, ceruloplasmin, complement component 1, complement component 3, platelet derived growth factor, integrin alpha 6, lysosomal acid lipase 1, β-2 micro globulin, ubiquitin, macrophage migration inhibitory factor, retinoic acid, cofilin, cyclophillin A, FKBP12, NDPK, profilin 1, cystatin C, calcyclin and insulin-like growth factor-1."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The cardiogenic cocktail of any of claims 1-6, further comprising adding one or more components to said cardiogenic cocktail, wherein said one or more components are selected from the group consisting of IGF-1, IL-6, FGF-4, TGF-β, BMP, LIF, and hα-thrombin."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The cardiogenic cocktail of any of claims 1-7, wherein said cardiogenic cocktail, upon exposure to embryonic stem cells, causes said embryonic stem cells to lose their tumorigenicity."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The cardiogenic cocktail of any of claims 1-8, wherein said cardiogenic cocktail, upon exposure to embryonic stem cells, causes said embryonic stem cells to differentiate into cardiopoietic stem cells."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["A cardiopoietic stem cell, obtainable by exposing embryonic stem cells to the cardiogenic cocktail of any of claims 1-8, wherein said cardiopoietic stem cell exhibits nuclear translocation of cardiac transcription factors, wherein cardiac transcription factors are NKX2.5, MEF2C, and GATA4; and wherein said cardiopoietic stem cell does not comprise sarcomeric proteins."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["A cardiopoietic stem cell, wherein said cardiopoietic stem cell expresses at least one polypeptide selected from the group consisting of integral membrane protein 2A; insulin-like growth factor binding protein 4; thymus cell antigen 1, theta; selenoprotein P, plasma, 1; and glycoprotein 38."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["A method of making the cardiopoietic stem cells of any of claims 9-11, comprising:
contacting a culture of embryonic stem cells with the cardiogenic cocktail of any of claims 1-8, under conditions wherein said cardiopoietic stem cells are formed in said culture, said cardiogenic cocktail comprising TGG-β1, TGF-β2, BMP-1, BMP-2, BMP-5, BMP-6, FGF-4, FGF-5, FGF-12, FGF-13, FGF-15, FGF-20, leukemia inhibitory factor, VEGF-C, insulin growth factor-1 and interleukin 6."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12, further comprising:
separating said cardiopoietic stem cells from said embryonic stem cells or from cardiomyocytes."],"number":13,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}