Qualitative/quantitative Detection Of Fungal Species

Abstract

A method based on species-specific molecular markers applied to a multiplex platform system is provided that permits, in a one-time assay, the qualitative and quantitative screening of multi-species populations in several dozens of environmental samples, tested in parallel, with low cost and rapid turn-around. The method identifies a specific portion of DNA, shared amongst a variety of important mold species, but with sufficient sequence differences that allow for unique identifications. Two pairs of primers, which are capable of amplifying this region across at least 38 different mold species, have been identified. Also short oligonucleotide probes have been designed specifically for each species to detect their presence and concentration in mixed species environmental samples. The method of detection of the invention can be practiced using conventional PCR reactions, followed by nucleotide hybridization assays and quantization of the hybridized biotinylated amplicons in a multiplex liquid array system.


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Document History
  • Publication: Sep 29, 2015
  • Application: Nov 23, 2011
    US US 201113303237 A
  • Priority: Nov 23, 2011
    US US 201113303237 A
  • Priority: Nov 24, 2010
    US US 41680010 P

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