Near-infrared Light-activated Proteins

Abstract

Methods and constructs are provided for controlling processes in live animal, plant or microbial organisms via genetically engineered near-infrared light-activated or light- inactivated proteins. In embodiments, these proteins are chimeras comprised of the photosensory modules of bacteriohytochromes and output modules that possess enzymatic activity and/or ability to bind to DNA, RNA, protein. or small molecules. DNA encoding these proteins can be introduced as genes into live animals, plants or microbes, where their activities can be turned on by near-infrared light, controlled by the intensity of light, and turned off by near-infrared light or a different wavelength than the activating light. By using light-inducible proteins, one can regulate diverse cellular processes with high spatial and temporal precision, in a nontoxic manner, often using external light sources. For example, near-infrared light-activated proteins possessing nucleotidyl cyclase, protein kinase, protease, DNA-binding and RNA-binding activities can be used to control signal transduction, cell apoptosis, proliferation, adhesion, differentiation and other cell processes.


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Document History
  • Publication: Jan 31, 2013
  • Application: Jul 27, 2012
    WO US 2012/0048699 W
  • Priority: Jul 27, 2011
    US US 201161512065 P

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