{"search_session":{},"preferences":{"l":"ru","queryLanguage":"ru"},"patentId":"042-821-194-959-190","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"042-821-194-959-190"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11706,"type":"PATENT","title":"The University of Georgia - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":3798,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8360,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: uni* georgia
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Add to collection. Total patents: 3820
comprises a knock-out of the coding region of a lactate dehydrogenase and produces lactate in an amount less than a comparable wild type control;\n
comprises at least one heterologous polynucleotide that encodes an enzyme in a metabolic pathway that converts acetyl-CoA to ethanol, wherein the enzyme is an acetaldehyde dehydrogenase that can convert acetyl-CoA to acetaldehyde, an aldehyde dehydrogenase that can convert acetyl-CoA to acetaldehyde, or a combination thereof; and wherein the Caldicellulosiruptor microbe is C. bescii, C. kristjansonii, C. obsidiansis, C. hydrothermalis, C. kronotskvensis, C. lactoaceticus, or C. saccharolvticus."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The recombinant Caldicellulosiruptor microbe of claim 1 wherein the Caldicellulosiruptor microbe is Caldicellulosiruptor bescii. "],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The recombinant Caldicellulosiruptor microbe of claim 1 comprising a deletion of at least a portion of a lactate dehydrogenase coding region."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The recombinant Caldicellulosiruptor microbe of claim 3 wherein the lactate dehydrogenase coding region encodes an NADH-dependent lactate dehydrogenase (Cbes 1918)."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The recombinant Caldicellulosiruptor microbe of claim 1 further genetically modified to exhibit decreased activity of an endonuclease that digests unmethylated DNA in a Caldicellulosiruptor spp. (CbeI) compared to a comparable wild-type control."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The recombinant Caldicellulosiruptor microbe of claim 1 further genetically modified to exhibit increased efficiency of transformation with unmethylated heterologous DNA."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["A method comprising:\n
growing the recombinant Caldicellulosiruptor microbe of claim 1, wherein the Caldicellulosiruptor microbe is genetically modified to produce a greater amount of ethanol than a comparable wild type control under conditions effective for the recombinant Caldicellulosiruptor microbe to produce ethanol, wherein the Caldicellulosiruptor microbe:\n
comprises a knock-out of the coding region of a lactate dehydrogenase and produces lactate in an amount less than a comparable wild type control;\n
comprises at least one heterologous polynucleotide that encodes an enzyme in a metabolic pathway that converts acetyl-CoA to ethanol, wherein the enzyme is an acetaldehyde dehydrogenase that can convert acetate to acetaldehyde, an aldehyde dehydrogenase that can convert acetyl-CoA to acetaldehyde, or a combination thereof; and wherein the\nCaldicellulosiruptor microbe is C. bescii, C. kristjansonii, C. obsidiansis, C. hydrothermalis, C. kronotskvensis, C. lactoaceticus, or C. saccharolvticus. "],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 7 further comprising collecting at least a portion of the ethanol."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 7 wherein the conditions comprise a carbon source that comprises napier grass, Bermuda grass, switchgrass, or a hardwood."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 9 wherein the carbon source comprises unpretreated switchgrass."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The recombinant Caldicellulosiruptor microbe of claim 1 further comprising a knock-out of the coding region of an acetate kinase where the microbe further comprises an alcohol dehydrogenase that converts acetaldehyde to ethanol."],"number":11,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}