{"search_session":{},"preferences":{"l":"zh","queryLanguage":"zh"},"patentId":"035-674-912-865-623","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"035-674-912-865-623"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8903,"type":"PATENT","title":"Johns Hopkins Univ Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":17938,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8218,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search applicants and owners= \"Johns Hopkins Univ\", \"Univ Johns Hopkins\"
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Total patents: 12941
Search applicants and owners= \"Johns Hopkins Univ\", \"Univ Johns Hopkins\"
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Total patents: 12941
expressing a construct in a cell, wherein the construct comprises the autoregulatory molecule, a measurable marker, and a cleavable substrate in a single molecule;\n
expressing a protease capable of cleaving the cleavable substrate in the cell, wherein the protease cleaves the cleavable substrate during translation allowing the autoregulatory molecule to fold into a functional molecule; and\n
evaluating the cell for the presence of the measurable substrate."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the cell is an E. coli cell."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the autoregulatory molecule is CI."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the measurable marker is selected from the group consisting of fluorescent peptides, colorimetric compounds, chemiluminescent peptides, and combinations thereof."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 5, where the fluorescent peptide is selected from the group consisting of yellow fluorescent protein (YFP), blue fluorescent protein (BFP), green fluorescent protein (GFP), red fluorescent protein (RFP) and fluorescing mutants thereof."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["A purified and isolated polynucleotide, comprising\n
a polynucleotide sequence encoding an autoregulatory molecule,\n
a polynucleotide sequence encoding a measurable marker, and\n
a polynucleotide sequence encoding a cleavable substrate, wherein the polynucleotide sequence encoding the cleavable substrate connects the polynucleotide sequence encoding the autoregulatory molecule and the polynucleotide sequence encoding the measurable marker, and wherein upon translation of the polynucleotide, when expressed in a cell, the cleavable substrate is cleaved by a protease releasing the measurable marker and allowing the autoregulatory molecule to fold functionally."],"number":6,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}