Genes From Propionibacterium Freudenreichii Encoding Enzymes Involved In Vitamin B12 Biosynthesis


Four new genes, and the enzymes that they encode, are disclosed, which are present in Propionibacterium freudenreichii, and which are involved in at least five steps in the biosynthetic pathway of vitamin B12. The four enzymes are: A: cobyrinic acid a, c-diamide synthase; B: a bifunctional enzyme, which is a cobinamide kinase and a cobinamide phosphate guanyl transferase; C: a cobalamin 5-phosphate synthase; and D: an adenosyl transferase. Genes encoding the four enzymes can be placed in Propionibacteria shuttle vectors and used to transform Propionibacteria hosts, in order to improve the production of vitamin B12 or a precursor thereof on an industrial scale during fermentation.

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Document History
  • Publication: May 5, 2009
  • Application: Jan 25, 2005
    US US 52238905 A
  • Priority: Jul 25, 2003
    EP EP 0308216 W
  • Priority: Jul 25, 2002
    EP EP 02255203 A

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