{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"032-788-739-992-667","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"032-788-739-992-667"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10787,"type":"PATENT","title":"Rutgers University - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":7108,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8257,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: \"Rutgers Univ*\";
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 4935
Search Applicants and Owners separately: \"Rutgers Univ*\";
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 4935
a) providing seeds from a plant comprising an RNAi construct which acts in a dominant fashion to down modulate expression of alpha zeins, said down modulation decreasing the vitreous nature of seeds obtained from said plant;\n
b) propagating said seed from a) and crossing the resulting plant with a QPM plant line;\n
c) obtaining seeds from plants resulting from said cross and harvesting those kernels which exhibit an increased kernel vitreousness when compared to seeds in a) thereby providing a dominant selectable phenotype for elevated lysine and rapid selection of QPM modifiers."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, further comprising crossing plants obtained from the kernels of c) with a non-QPM line thereby promoting introgression of the QPM trait into said non-QPM line."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said reduced expression of the zein protein results in an increase in the seed of an amount of at least one amino acid which is essential to the diet of animals and humans."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said RNAi construct is introduced into said plant cell with at least one selectable marker and/or reporter gene thereby facilitating identification of cells comprising said RNAi construct."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said alpha-zein expression is reduced in said cell via introduction of analpha zeinRNAi construct."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1 further comprising breeding a transgenic plant from the harvested kernels to yield a progeny plant that has an increase in the amount of at least one amino acid as a dominant trait, said method optionally comprising backcrossing said progeny plant to said non-QPM parent containing a traditional o2 mutation to eliminate the RNAitransgene construct, thereby replacing it with the non-transgenic o2 mutation."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["A plant obtained by the method of claim 5."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["A method for production of improved maize seed comprising:\n
a) providing seeds from a plant comprising an RNAi construct which acts in a dominant fashion to down modulate expression of alpha zeins, said down modulation decreasing the vitreous nature of seeds obtained from said plant, said RNAi construct optionally comprising a nucleic acid encoding a reporter molecule;\n
b) propagating said seed from a) and crossing the resulting plant with an IHP QPM maize line;\n
c) obtaining seeds from plants resulting from said cross and harvesting those kernels which exhibit expression of said reporter molecule, said seeds exhibiting an increased kernel vitreousness, and elevated protein content when compared to seeds in a) or seeds obtained from IHP maize."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 8, wherein said seed from step c) exhibits elevated lysine content."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 8, wherein said seed from step c) exhibits elevated tryptophan content."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 8, wherein said seed from step c) exhibits elevated methionine content."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 8, wherein said reporter gene is green fluorescent protein."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 8, wherein said RNAi construct is shown in FIG. 6."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 8, wherein said seed have the genotype IHP/−; P6z1RNAi/−."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["A plant obtained from the seed of claim 14."],"number":15,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}