Synthetic Gene For Enhanced Expression In E. Coli

  • Published: Feb 5, 2009
  • Earliest Priority: Jun 29 2007
  • Family: 3
  • Cited Works: 0
  • Cited by: 0
  • Cites: 8
  • Sequences: 22
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A novel nesiritide synthetic cDNA chimera encoding human b-type natriuretic peptide (hBNP) or nesiritide and a process for the preparation of the said novel chimera. Further, the inventors disclose the use of nesiritide synthetic cDNA chimera to obtain an expressible construct to produce mature nesiritide. Particularly, the inventors disclose an application of recombinant cloning method to prepare an ORF of a nesiritide chimeric construct, which is simultaneously codon optimized for E. coli and has optimal RNA stability. The inventors also provide a process for large scale purification of nesiritide by pH precipitation and chromatography.

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Document History
  • Publication: Feb 5, 2009
  • Application: May 23, 2008
    US US 12631908 A
  • Priority: Jun 29, 2007
    IN IN 1247MU2007 A

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