Muscle Sample Prepared For Prion Assay


A method of preparing a sample of muscle tissue and of assaying the prepared sample to determine the presence of prions in the sample is disclosed. The muscle tissue is homogenized and mixed with a complexing agent which forms a complex with a higher specific gravity than PrP<SUP>Sc</SUP>, the complexing agent or other components of the homogenate. Gravity is then used (e.g. ultra centrifugation) to concentrate the complex and the concentrate is assayed to detect prions. The muscle tissue is preferably extracted from a muscle or group of muscles such as hind limb muscle which have a higher or more preferably the highest concentration of prions as compared to other muscle in the mammal.

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Document History
  • Publication: Jan 23, 2007
  • Application: Aug 2, 2002
    US US 21194202 A
  • Priority: Aug 2, 2002
    US US 21194202 A
  • Priority: Jan 22, 2002
    US US 35152502 P
  • Priority: Sep 20, 2001
    US US 32390301 P

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