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Method For Identifying Phytochemical Type Of Marihuana By Virtue Of One-step Multiplex Pcr

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Abstract

The invention discloses a method for identifying a phytochemical type of a marihuana by virtue of one-step multiplex PCR. The method for identifying the phytochemical type of the marihuana by virtue of one-step multiplex PCR comprises the following steps: 1) extracting genome DNA of a marihuana plant; 2) designing a multiplex PCR primer; 3) preparing a PCR reaction system; 4) carrying out PCR amplification; 5) carrying out agarose electrophoresis; and 6) observing an electrophoretic band of a PCR product and judging a chemical type. By virtue of the one-step PCR reaction, a drug type marihuana can be amplified to obtain a specific THC stripe with the size being about 550bp, a fiber type marihuana can be amplified to obtain a CBD stripe with the size being about 1050bp, and an intermediate type marihuana can be amplified the THC stripe with the size being about 550bp and the CBD stripe with the size being about 1050bp at the same time, so that chemical type separation of a tested marihuana sample can be visually judged according to amplification stripes. The method for identifying the phytochemical type of the marihuana by virtue of the one-step multiplex PCR is not restricted by sampling time, a sampling site and the like in the traditional chemical detection, has the characteristics of short period, low cost and the like, is easy to operate, can be used for quickly identifying the phytochemical type of the marihuana and can be used by a public security drug detection department, an agriculture breeding department and the like, so that the method for identifying the phytochemical type of the marihuana by virtue of the primary multiplex PCR has a good application prospect.


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Document History
  • Publication: Jan 21, 2015
  • Application: Sep 28, 2014
    CN CN 201410505113 A
  • Priority: Sep 28, 2014
    CN CN 201410505113 A

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