{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"013-063-029-707-73X","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"013-063-029-707-73X","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8906,"type":"PATENT","title":"Univ Pennsylvania Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":24238,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8221,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search applicants and owners= \"Univ Pennsylvania\", \"Pennsylvania Univ\", \"Univ Penns*\", \"Penn* Univ\", \"University of Pennsylvania\", \"Pennsylvania University\".
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Search applicants and owners= \"Univ Pennsylvania\", \"Pennsylvania Univ\", \"Univ Penns*\", \"Penn* Univ\", \"University of Pennsylvania\", \"Pennsylvania University\".
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1) contacting a plurality of immortalized cells in vitro that are deficient in Bax gene expression and Bak gene expression with a test compound;\n
2) determining if PARP is activated in said cells contacted with said test compound wherein the activation of PARP in said cells contacted with said test compound indicates that the test compound can induce cell death by necrosis in cells in vitro which rely on catabolizing glucose for ATP production."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said measuring PARP activation comprises measuring PAR polymers, NAD depletion, or ATP depletion."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, further comprising performing a negative control assay which comprises determining if PARP is activated in a plurality of bax−/−bak−/− immortalized cells in the absence of the test compound."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, further comprising performing a positive control assay which comprises determining if PARP is activated in a plurality of bax−/−bak−/− immortalized cells with a positive control compound and determining if PARP is activated."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the PARP activation in said cells contacted with said test compound is at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 100%, or at least 200% greater than the PARP activation in said cells in the absence of said test compound."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["A method of identifying a compound which can induce cell death by necrosis in cells which rely on catabolizing glucose for ATP production in vitro comprising, the steps of:\n
1) contacting a plurality of immortalized cells in vitro that are deficient in Bax gene expression and Bak gene expression with a test compound;\n
2) determining if PARP is activated in said cells contacted with said test compound;\n
3) determining if necrosis is induced in said cells;\n
wherein the activation of PARP and induction of necrosis in said cells contacted with said test compound indicates that the test compound-can induce cell death by necrosis in cells in vitro which rely on catabolizing glucose for ATP production."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6, wherein said measuring PARP activation comprises measuring PAR polymers, NAD depletion, or ATP depletion."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6, further comprising performing a negative control assay which comprises determining if PARP is activated in a plurality of bax−/−bak−/− immortalized cells in the absence of the test compound."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6, further comprising performing a positive control assay which comprises determining if PARP is activated in a plurality of bax−/−bak−/− immortalized cells with a positive control compound and determining if PARP is activated."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6, wherein the PARP activation in said cells contacted with said test compound is at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 100%, or at least 200% greater than the PARP activation in said cells in the absence of said test compound."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6, wherein necrosis is determined by measuring organelle swelling, intracellular vacuole formation, plasma membrane disintegration, or nuclear degradation without condensation."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["A method of identifying a compound that can induce necrosis in cells which rely on catabolizing glucose for ATP production in vitro comprising:\n
1) contacting a plurality of immortalized cells in vitro that are deficient in Bax gene expression and Bak gene expression with a test compound; and\n
2) determining if necrosis is induced in said cells contacted with said test compound;\n
wherein induction of necrosis in said cells contacted with said test compound indicates that the test compound-can induce cell death by necrosis in cells in vitro which rely on catabolizing glucose for ATP production."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12, wherein necrosis is determined by measuring organelle swelling, intracellular vacuole formation, plasma membrane disintegration, or nuclear degradation without condensation."],"number":13,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}