{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"011-927-566-137-528","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"011-927-566-137-528"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":22728,"type":"PATENT","title":"Citing UC Davis publications","description":"Patent documents citing scholarly work of UC Davis","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":34174,"tags":[],"user":{"id":233682368,"username":"tech","firstName":"The Lens","lastName":"Team","created":"2017-08-06T20:11:49.000Z","displayName":"The Lens Team","profilePictureKey":"lens/users/15eac2a0-031d-4923-92cb-a162e1cb2bbb/profile-picture","preferences":"{\"beta\":true}","accountType":"PERSONAL","isOauthOnly":false},"notes":[],"sharedType":"PUBLISHED","hasLinkedSavedQueries":false,"savedQueries":[],"created":"2017-08-07T04:41:48Z","updated":"2017-08-07T04:41:48Z","lastEventDate":"2017-08-07T04:41:48Z"},{"id":22802,"type":"PATENT","title":"Citing UC System publications","description":"Patent documents citing scholarly work of UC System","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":266608,"tags":[],"user":{"id":233682368,"username":"tech","firstName":"The Lens","lastName":"Team","created":"2017-08-06T20:11:49.000Z","displayName":"The Lens Team","profilePictureKey":"lens/users/15eac2a0-031d-4923-92cb-a162e1cb2bbb/profile-picture","preferences":"{\"beta\":true}","accountType":"PERSONAL","isOauthOnly":false},"notes":[],"sharedType":"PUBLISHED","hasLinkedSavedQueries":false,"savedQueries":[],"created":"2017-08-07T05:10:38Z","updated":"2017-08-07T05:10:38Z","lastEventDate":"2017-08-07T05:10:38Z"},{"id":22859,"type":"PATENT","title":"Citing Harvard Univ publications","description":"Patent documents citing scholarly work of Harvard Univ","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":175224,"tags":[],"user":{"id":233682368,"username":"tech","firstName":"The Lens","lastName":"Team","created":"2017-08-06T20:11:49.000Z","displayName":"The Lens Team","profilePictureKey":"lens/users/15eac2a0-031d-4923-92cb-a162e1cb2bbb/profile-picture","preferences":"{\"beta\":true}","accountType":"PERSONAL","isOauthOnly":false},"notes":[],"sharedType":"PUBLISHED","hasLinkedSavedQueries":false,"savedQueries":[],"created":"2017-08-07T05:37:44Z","updated":"2017-08-07T05:37:44Z","lastEventDate":"2017-08-07T05:37:44Z"}],"notes":[],"inventorships":[],"privateCollections":[],"publicCollections":[{"id":22728,"type":"PATENT","title":"Citing UC Davis publications","description":"Patent documents citing scholarly work of UC Davis","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":34174,"tags":[],"user":{"id":233682368,"username":"tech","firstName":"The Lens","lastName":"Team","created":"2017-08-06T20:11:49.000Z","displayName":"The Lens Team","profilePictureKey":"lens/users/15eac2a0-031d-4923-92cb-a162e1cb2bbb/profile-picture","preferences":"{\"beta\":true}","accountType":"PERSONAL","isOauthOnly":false},"notes":[],"sharedType":"PUBLISHED","hasLinkedSavedQueries":false,"savedQueries":[],"created":"2017-08-07T04:41:48Z","updated":"2017-08-07T04:41:48Z","lastEventDate":"2017-08-07T04:41:48Z"},{"id":22802,"type":"PATENT","title":"Citing UC System publications","description":"Patent documents citing scholarly work of UC System","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":266608,"tags":[],"user":{"id":233682368,"username":"tech","firstName":"The Lens","lastName":"Team","created":"2017-08-06T20:11:49.000Z","displayName":"The Lens Team","profilePictureKey":"lens/users/15eac2a0-031d-4923-92cb-a162e1cb2bbb/profile-picture","preferences":"{\"beta\":true}","accountType":"PERSONAL","isOauthOnly":false},"notes":[],"sharedType":"PUBLISHED","hasLinkedSavedQueries":false,"savedQueries":[],"created":"2017-08-07T05:10:38Z","updated":"2017-08-07T05:10:38Z","lastEventDate":"2017-08-07T05:10:38Z"},{"id":22859,"type":"PATENT","title":"Citing Harvard Univ publications","description":"Patent documents citing scholarly work of Harvard Univ","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":175224,"tags":[],"user":{"id":233682368,"username":"tech","firstName":"The Lens","lastName":"Team","created":"2017-08-06T20:11:49.000Z","displayName":"The Lens Team","profilePictureKey":"lens/users/15eac2a0-031d-4923-92cb-a162e1cb2bbb/profile-picture","preferences":"{\"beta\":true}","accountType":"PERSONAL","isOauthOnly":false},"notes":[],"sharedType":"PUBLISHED","hasLinkedSavedQueries":false,"savedQueries":[],"created":"2017-08-07T05:37:44Z","updated":"2017-08-07T05:37:44Z","lastEventDate":"2017-08-07T05:37:44Z"}],"privateNotes":[],"landscapeCollections":[],"landscapeNotes":[]},"document":{"record_lens_id":"011-927-566-137-528","lens_id":["011-927-566-137-528","125-835-945-776-202"],"doc_key":"US_20130031965_A1_20130207","created":"2016-01-15T11:55:29.715","docdb_id":380923591,"lens_internal":{"earliest_lens_id_created_time":"2016-01-15T11:55:29.715","last_modified":"2024-03-25T22:38:24.692","legacy_pub_key":"US_2013_0031965_A1","has_doc_lang":true,"has_biblio_lang":true,"has_all_title_lang":true,"has_all_abstract_lang":true,"has_all_claims_lang":true,"has_description_lang":true},"jurisdiction":"US","doc_number":"20130031965","kind":"A1","date_published":"2013-02-07","year_published":2013,"ids":["US_2013_0031965_A1","011-927-566-137-528","125-835-945-776-202","US_20130031965_A1_20130207","US","20130031965","A1","US20130031965A1","US20130031965","20130031965A1"],"lang":"en","publication_type":"PATENT_APPLICATION","application_reference":{"jurisdiction":"US","doc_number":"201213426891","kind":"A","date":"2012-03-22"},"priority_claim":[{"jurisdiction":"US","doc_number":"201213426891","kind":"A","date":"2012-03-22"},{"jurisdiction":"US","doc_number":"201161513921","kind":"P","date":"2011-08-01"}],"priority_claim.source":"DOCDB","earliest_priority_claim_date":"2011-08-01","title":{"en":[{"text":"METHOD OF TESTING FOR A MILK TRISACCHARIDE","lang":"en","source":"DOCDB","data_format":"DOCDBA"}]},"title_lang":["en"],"has_title":true,"applicant":[{"name":"MADSON MICHAEL","residence":"US","sequence":1,"app_type":"applicant"}],"applicant_count":1,"has_applicant":true,"inventor":[{"name":"MADSON MICHAEL","residence":"US","sequence":1}],"inventor_count":1,"has_inventor":true,"agent":[],"agent_count":0,"has_agent":false,"owner":[{"name":"BIOLOGISTICS LLC","address":"P.O. BOX 0183, GARNER, IOWA, 50438","sequence":1,"recorded_date":"2012-04-26","execution_date":"2012-04-20","is_current_owner":true}],"owner_count":1,"owner_all":[{"name":"BIOLOGISTICS LLC","address":"P.O. BOX 0183, GARNER, IOWA, 50438","sequence":1,"recorded_date":"2012-04-26","execution_date":"2012-04-20","is_current_owner":true}],"owner_all_count":1,"has_owner":true,"has_examiner":false,"class_ipcr":[{"symbol":"G01N30/00","version_indicator":"2006-01-01","class_symbol_position":"F","class_value":"I","action_date":"2013-02-07","class_status":"B","class_data_source":"H","generating_office":"US","sequence":1}],"class_ipcr.first_symbol":"G01N30/00","class_ipcr.later_symbol":[],"class_ipcr.inv_symbol":["G01N30/00"],"class_ipcr.add_symbol":[],"class_ipcr.source":"DOCDB","class_cpc":[{"symbol":"G01N33/04","version_indicator":"2013-01-01","class_symbol_position":"F","class_value":"I","action_date":"2013-09-30","class_status":"B","class_data_source":"H","generating_office":"EP","sequence":1},{"symbol":"G01N33/04","version_indicator":"2013-01-01","class_symbol_position":"F","class_value":"I","action_date":"2013-09-30","class_status":"B","class_data_source":"H","generating_office":"US","sequence":2},{"symbol":"G01N2030/8836","version_indicator":"2013-01-01","class_symbol_position":"L","class_value":"A","action_date":"2013-09-30","class_status":"B","class_data_source":"H","generating_office":"EP","sequence":3},{"symbol":"G01N2030/8836","version_indicator":"2013-01-01","class_symbol_position":"L","class_value":"A","action_date":"2013-09-30","class_status":"B","class_data_source":"H","generating_office":"US","sequence":4}],"class_cpc_cset":[],"class_cpc.first_symbol":"G01N33/04","class_cpc.later_symbol":["G01N2030/8836","G01N2030/8836"],"class_cpc.inv_symbol":["G01N33/04","G01N33/04"],"class_cpc.add_symbol":["G01N2030/8836","G01N2030/8836"],"class_cpc.source":"DOCDB","class_national":[{"symbol":"73/61.52","symbol_position":"F"},{"symbol":"73/61.43","symbol_position":"L"}],"class_national.first_symbol":"73/61.52","class_national.later_symbol":["73/61.43"],"class_national.source":"DOCDB","reference_cited":[{"patent":{"num":1,"document_id":{"jurisdiction":"US","doc_number":"2009104603","kind":"A1","date":"2009-04-23","name":"SATOMAA TERO [FI], et al"},"lens_id":"066-445-429-471-144","category":[],"us_category":[],"cited_phase":"PRS","rel_claims":[],"sequence":1}},{"npl":{"num":1,"text":"Tao et al., Journal of Dairy Science, Vol. 91, 2008, pages 3768 - 3778","npl_type":"a","external_id":["10.3168/jds.2008-1305","18832198"],"record_lens_id":"058-959-086-840-136","lens_id":["119-701-850-263-796","085-431-694-781-735","058-959-086-840-136"],"sequence":2,"category":[],"us_category":[],"cited_phase":"PRS","rel_claims":[]}},{"npl":{"num":2,"text":"Tao et al, Journal of Dairy Science, Vol 92, 2009, pages 2991 - 3001","npl_type":"a","external_id":["10.3168/jds.2008-1642","19528576"],"record_lens_id":"005-817-096-396-212","lens_id":["146-025-566-635-87X","116-294-222-247-176","005-817-096-396-212"],"sequence":3,"category":[],"us_category":[],"cited_phase":"PRS","rel_claims":[]}},{"npl":{"num":3,"text":"Cataldi et al., Analytica Chimica Acta, Volume 485, Issue 1, 26 May 2003, Pages 43 - 49","npl_type":"a","external_id":[],"lens_id":[],"sequence":4,"category":[],"us_category":[],"cited_phase":"PRS","rel_claims":[]}},{"npl":{"num":4,"text":"Stepans et al. Breastfeeding Medicine, Volume 1, Number 4, 2006","npl_type":"a","external_id":["10.1089/bfm.2006.1.207","17661601"],"record_lens_id":"073-242-738-633-198","lens_id":["182-635-519-373-866","073-242-738-633-198","189-439-483-634-310"],"sequence":5,"category":[],"us_category":[],"cited_phase":"PRS","rel_claims":[]}},{"npl":{"num":5,"text":"Ward, Open Glycoscience, 2009, 2, 9-15","npl_type":"a","external_id":[],"lens_id":[],"sequence":6,"category":[],"us_category":[],"cited_phase":"PRS","rel_claims":[]}},{"npl":{"num":6,"text":"Messer, Biochem. J. (1974) 139, 415-420","npl_type":"a","external_id":["10.1042/bj1390415","4374932","pmc1166298"],"record_lens_id":"109-083-757-411-688","lens_id":["116-861-704-326-457","109-083-757-411-688","160-063-220-292-026"],"sequence":7,"category":[],"us_category":[],"cited_phase":"PRS","rel_claims":[]}}],"reference_cited.source":"DOCDB","reference_cited.patent_count":1,"cites_patent":true,"reference_cited.npl_count":6,"reference_cited.npl_resolved_count":4,"cites_npl":true,"cites_resolved_npl":true,"cited_by":{"patent_count":1,"patent":[{"lens_id":"147-499-622-047-150","document_id":{"jurisdiction":"CN","doc_number":"105424858","kind":"A"}}]},"cited_by_patent":true,"family":{"simple":{"size":2,"id":245198728,"member":[{"lens_id":"011-927-566-137-528","document_id":{"jurisdiction":"US","doc_number":"20130031965","kind":"A1","date":"2013-02-07"}},{"lens_id":"088-269-445-674-185","document_id":{"jurisdiction":"US","doc_number":"8993226","kind":"B2","date":"2015-03-31"}}]},"extended":{"size":6,"id":243321449,"member":[{"lens_id":"183-047-684-088-255","document_id":{"jurisdiction":"US","doc_number":"9557335","kind":"B2","date":"2017-01-31"}},{"lens_id":"130-808-039-992-197","document_id":{"jurisdiction":"US","doc_number":"20150323540","kind":"A1","date":"2015-11-12"}},{"lens_id":"011-927-566-137-528","document_id":{"jurisdiction":"US","doc_number":"20130031965","kind":"A1","date":"2013-02-07"}},{"lens_id":"199-104-525-794-59X","document_id":{"jurisdiction":"US","doc_number":"9726671","kind":"B2","date":"2017-08-08"}},{"lens_id":"105-920-600-245-458","document_id":{"jurisdiction":"US","doc_number":"20150064793","kind":"A1","date":"2015-03-05"}},{"lens_id":"088-269-445-674-185","document_id":{"jurisdiction":"US","doc_number":"8993226","kind":"B2","date":"2015-03-31"}}]}},"has_sequence":false,"legal_status":{"ipr_type":"patent for invention","granted":true,"earliest_filing_date":"2012-03-22","grant_date":"2015-03-31","anticipated_term_date":"2032-03-22","discontinuation_date":"2023-05-08","has_disclaimer":false,"patent_status":"INACTIVE","publication_count":2,"has_spc":false,"has_grant_event":true,"has_entry_into_national_phase":false},"abstract":{"en":[{"text":"A method of testing for a trisaccharide in milk includes the step of providing a predetermined amount of milk. The method also includes the step of isolating a trisachharide of the milk with a hydride insertion reaction to identify the molecule as phosphorylated. Also included in the method is the step of obtaining a mass spectrum of the isolated milk trisaccharide sample. The method further includes the step of identifying a molecular structure of the isolated milk trisaccharide sample.","lang":"en","source":"USPTO_FULLTEXT","data_format":"ORIGINAL"}]},"abstract_lang":["en"],"has_abstract":true,"claim":{"en":[{"text":"1 . A method of testing for a trisaccharide in milk comprising the steps of: providing a predetermined amount of milk; isolating a trisachharide of the milk to obtain a sample; obtaining a mass spectrum of the isolated milk trisaccharide sample; and identifying a molecular structure of the isolated milk trisaccharide sample.","lang":"en","source":"USPTO_FULLTEXT","data_format":"ORIGINAL"},{"text":"2 . The method of testing for a trisaccharide in milk of claim 1 additionally comprising the step of subjecting the isolated milk trisaccharide sample to high performance chromatography.","lang":"en","source":"USPTO_FULLTEXT","data_format":"ORIGINAL"},{"text":"3 . The method of testing for a trisaccharide in milk of claim 1 wherein the hydride reduction is comprised of the steps of combining the predetermined amount of milk with ethanol and centrifuging the milk and ethanol to form a supernatant.","lang":"en","source":"USPTO_FULLTEXT","data_format":"ORIGINAL"},{"text":"4 . The method of testing for a trisaccharide in milk of claim 3 wherein the hydride reduction is used to determine phosphorylation.","lang":"en","source":"USPTO_FULLTEXT","data_format":"ORIGINAL"},{"text":"5 . The method of testing for a trisaccharide in milk of claim 4 wherein the milk is pasteurized fat free bovine milk.","lang":"en","source":"USPTO_FULLTEXT","data_format":"ORIGINAL"},{"text":"6 . The method of testing for a trisaccharide in milk of claim 5 wherein the ethanol is cold 95% ethanol.","lang":"en","source":"USPTO_FULLTEXT","data_format":"ORIGINAL"},{"text":"7 . The method of testing for a trisaccharide in milk of claim 6 wherein ethanol is combined with the predetermined amount of milk at a 10:1 ratio.","lang":"en","source":"USPTO_FULLTEXT","data_format":"ORIGINAL"},{"text":"8 . The method of testing for a trisaccharide in milk of claim 4 wherein the supernatant is comprised of the isolated milk trisaccharide sample.","lang":"en","source":"USPTO_FULLTEXT","data_format":"ORIGINAL"},{"text":"9 . The method of testing for a trisaccharide in milk of claim 8 further comprising the step of identifying a structure of a molecular ion of the isolated milk trisaccharide sample.","lang":"en","source":"USPTO_FULLTEXT","data_format":"ORIGINAL"},{"text":"10 . The method of testing for a trisaccharide in milk of claim 9 further comprising the step of identifying a substitution position of a phosphate from the structure of the molecular ion of the isolated milk trisaccharide sample.","lang":"en","source":"USPTO_FULLTEXT","data_format":"ORIGINAL"},{"text":"11 . The method of testing for a trisaccharide in milk of claim 9 further comprising the step of determining whether the trisaccharide molecule is phosphorylated based upon the molecular structure of the isolated milk trisaccharide sample.","lang":"en","source":"USPTO_FULLTEXT","data_format":"ORIGINAL"}]},"claim_lang":["en"],"has_claim":true,"description":{"en":{"text":"CROSS REFERENCE TO RELATED APPLICATIONS This application claims the benefit of U.S. Provisional Application No. 61/513,921, filed Aug. 1, 2011. BACKGROUND OF THE INVENTION This invention relates to a method of testing for a milk trisaccharide. More specifically, the present invention relates to a laboratory method that can be used in order to isolate a bovine milk trisaccharide. Cancer is a disease that affects millions of families and individuals every year. As a result scientists are in a continuous search for substances that are effective in promoting anti-cancer activity whether the substances prevent the onset of cancer or alternatively slow down or stop the growth of cancer. Thus, a principal object of the present invention is to provide a method for isolating a bovine milk trisaccharide in order to determine the trisaccharide's anti-cancer activity. SUMMARY OF THE INVENTION A method of testing for a trisaccharide in milk. The method includes the step of providing a predetermined amount of milk. The method also includes the step of isolating a trisachharide of the milk, to obtain a sample, and by hydride insertion to help identify the milk trisaccharide. Also included in the method is the step of obtaining a mass spectrum of the isolated milk trisaccharide sample. The method further includes the step of identifying a molecular structure of the isolated milk trisaccharide sample. BRIEF DESCRIPTION OF DRAWINGS FIG. 1 is a perspective view of the system of isolating a milk trisaccharide of the present invention; FIG. 2 is a diagram of the fragmentation of the complete molecular ion of a sample of milk trisaccharide identified and isolated by the system of testing for a milk trisaccharide of the present invention; and FIG. 3 is a diagram of a mass spectrum of a sample of the isolated milk trisaccharide identified by the system of testing for a milk trisaccharide of the present invention. DESCRIPTION OF THE PREFERRED EMBODIMENT The figures show a system 10 utilized in order to isolate a bovine milk trisaccharide. The system 10 includes a pipet 12 , first and second centrifuge tubes, 14 , 16 , that in a preferred embodiment are plastic, and a centrifuge 18 having a cap 20 disposed thereon. In addition the system can include a freezer or a container holding water 22 . The method used to isolate the bovine milk trisaccharide using the system 10 is to pipet a predetermined amount of commercialized pasteurized cold fat free milk 24 into the centrifuge tube 14 . In a preferred embodiment 0.1 ml commercial pasteurized cold fat free milk 24 is pipet into a 15 ml plastic centrifuge tube 14 . Once the commercialized pasteurized cold fat free milk 24 is in the centrifuge tube 14 , 1 ml of cold 95% ethanol 26 is added. Thus, a 10:1 ratio of 95% ethanol 26 to the commercialized pasteurized cold fat free milk 24 is presented. At this time the centrifuge tube 18 is capped and centrifuged for a predetermined amount of time, preferably 30-40 seconds. After centrifuging the supernatant 28 is formed and is placed in a second centrifuge tube 16 . The supernatant 28 is then dried with a stream of nitrogen and placed in a freezer 22 for storage. The sample 28 can later be dissolved in water. A mass spectrum of this sample 28 presents a major ion, m/z 683.3 that is a cross ring cleavage product of the actual ion that is found in the original spectrum of milk fraction. M/Z 705.5 meanwhile is sodium salt as identified in FIG. 3 . Thus, this sample is a trisaccharide of bovine milk 30 that contains N-Acetyl neuraminic acid substituted lactosyl-6′ phosphate. From mass spectrometry, HPLC and a hydride reduction the trisaccharide was identified and the identity of the substitution position of phosphate is presented. Specifically, the molecule is phosphorylated that is discerned by utilizing a hydride reduction insertion. Thus, by isolating this milk trisaccharide, this milk fraction can be tested for anti cancer activity. Thus, at least all of the stated problems have been overcome.","lang":"en","source":"USPTO_FULLTEXT","data_format":"ORIGINAL"}},"description_lang":["en"],"has_description":true,"has_docdb":true,"has_inpadoc":true,"has_full_text":true,"biblio_lang":"en"},"jurisdiction":"US","collections":[],"usersTags":[],"lensId":"011-927-566-137-528","publicationKey":"US_2013_0031965_A1","displayKey":"US 2013/0031965 A1","docAssets":{"lensId":"011-927-566-137-528","pdfUrl":"https://www.lens.org/images/patent/US/20130031965/A1/US_2013_0031965_A1.pdf","images":[{"thumb":"https://s3-us-west-2.amazonaws.com/lens-resource/patent/US/A1/20130031/20130031965/image/page/10pc/00000001.png","full":"https://s3-us-west-2.amazonaws.com/lens-resource/patent/US/A1/20130031/20130031965/image/page/full/00000001.png"},{"thumb":"https://s3-us-west-2.amazonaws.com/lens-resource/patent/US/A1/20130031/20130031965/image/page/10pc/00000002.png","full":"https://s3-us-west-2.amazonaws.com/lens-resource/patent/US/A1/20130031/20130031965/image/page/full/00000002.png"},{"thumb":"https://s3-us-west-2.amazonaws.com/lens-resource/patent/US/A1/20130031/20130031965/image/page/10pc/00000003.png","full":"https://s3-us-west-2.amazonaws.com/lens-resource/patent/US/A1/20130031/20130031965/image/page/full/00000003.png"},{"thumb":"https://s3-us-west-2.amazonaws.com/lens-resource/patent/US/A1/20130031/20130031965/image/page/10pc/00000004.png","full":"https://s3-us-west-2.amazonaws.com/lens-resource/patent/US/A1/20130031/20130031965/image/page/full/00000004.png"},{"thumb":"https://s3-us-west-2.amazonaws.com/lens-resource/patent/US/A1/20130031/20130031965/image/page/10pc/00000005.png","full":"https://s3-us-west-2.amazonaws.com/lens-resource/patent/US/A1/20130031/20130031965/image/page/full/00000005.png"}],"fallover":false},"countryName":"USA","inventorModel":{"inventors":[{"name":{"value":"MADSON MICHAEL","valueNormalised":"Madson Michael"},"inventorship":null}],"inventorships":[],"unmatchedInventorships":[],"activeUserHasInventorship":false},"simpleFamilyId":245198728,"citesPatentCount":1,"countrySpec":{"countryName":"USA","description":"FIRST PUBLISHED PATENT APPLICATION [FROM 2001 ONWARDS]","rule":"pubdate:AFTER:15-03-2001","docType":"PATENT_APPLICATION"},"pageTitle":"US 2013/0031965 A1 - Method Of Testing For A Milk Trisaccharide","documentTitle":"Method Of Testing For A Milk Trisaccharide"},"claims":{"source":"xml_claims","claims":[{"lines":["A method of testing for a trisaccharide in milk comprising the steps of:\n
providing a predetermined amount of milk;\n
isolating a trisachharide of the milk to obtain a sample;\n
obtaining a mass spectrum of the isolated milk trisaccharide sample; and\n
identifying a molecular structure of the isolated milk trisaccharide sample."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of testing for a trisaccharide in milk of claim 1 additionally comprising the step of subjecting the isolated milk trisaccharide sample to high performance chromatography."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of testing for a trisaccharide in milk of claim 1 wherein the hydride reduction is comprised of the steps of combining the predetermined amount of milk with ethanol and centrifuging the milk and ethanol to form a supernatant."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of testing for a trisaccharide in milk of claim 3 wherein the hydride reduction is used to determine phosphorylation."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of testing for a trisaccharide in milk of claim 4 wherein the milk is pasteurized fat free bovine milk."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of testing for a trisaccharide in milk of claim 5 wherein the ethanol is cold 95% ethanol."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of testing for a trisaccharide in milk of claim 6 wherein ethanol is combined with the predetermined amount of milk at a 10:1 ratio."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of testing for a trisaccharide in milk of claim 4 wherein the supernatant is comprised of the isolated milk trisaccharide sample."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of testing for a trisaccharide in milk of claim 8 further comprising the step of identifying a structure of a molecular ion of the isolated milk trisaccharide sample."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of testing for a trisaccharide in milk of claim 9 further comprising the step of identifying a substitution position of a phosphate from the structure of the molecular ion of the isolated milk trisaccharide sample."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of testing for a trisaccharide in milk of claim 9 further comprising the step of determining whether the trisaccharide molecule is phosphorylated based upon the molecular structure of the isolated milk trisaccharide sample."],"number":11,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}