In Vitro Mutagenesis, Phenotyping, And Gene Mapping

Abstract

Cellular libraries useful for in vitro phenotyping and gene mapping. In a representative approach, a method for preparing a homozygous cellular library includes the steps of providing a heterozygous cellular library comprising a plurality of isolated parent cells; inducing site-specific mitotic recombination in the plurality of isolated parent cells; culturing the plurality of isolated parent cells, whereby a population of daughter cells is produced; and selecting daughter cells comprising a homozygous genetic modification, whereby a homozygous cellular library is prepared.


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Document History
  • Publication: Apr 24, 2007
  • Application: May 2, 2003
    US US 42897703 A
  • Priority: May 2, 2003
    US US 42897703 A
  • Priority: May 2, 2002
    US US 37786402 P

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