Abstract
Transgenic plant cells and plants are described which synthesise a starch which is modified in comparison with corresponding wild type plant cells and plants. The plant cells and plants described show a reduced activity of R1, BEI and BEII proteins. Furthermore, modified starches as well as methods for their production are described.
Claims
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An isolated starch comprising an amylose content of at least 75% and a phosphate content which is reduced by at least 50% in comparison to starch from corresponding wild type potato plant cells,
wherein said starch is derived from transgenic potato plant cells comprising
(1) a first foreign nucleic acid molecule comprising
(a) a nucleic acid sequence comprising at least 500 nucleotides of a starch phosphorylating (R1) gene operably linked to a promoter in an antisense orientation leading to reduced expression of an endogenous gene encoding a R1 protein compared to expression in the non-transgenic potato plant cell;
(b) a nucleic acid sequence comprising at least 500 nucleotides of a starch phosphorylating (R1) gene which, via a co-suppression effect, reduces the expression of an endogenous gene encoding a R1 protein compared to expression in the non-transgenic potato plant cell; or
(c) a nucleic acid sequence comprising at least 500 nucleotides of a starch phosphorylating (R1) gene that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a R1 protein compared to expression in the non-transgenic potato plant cell;
(2) a second foreign nucleic acid molecule comprising
(a) a nucleic acid sequence comprising at least 500 nucleotides of a branching enzyme I (BEI) gene operably linked to a promoter in an antisense orientation leading to reduced expression of an endogenous gene encoding a BEI protein compared to expression in the non-transgenic potato plant cell;
(b) a nucleic acid sequence comprising at least 500 nucleotides of a branching enzyme I (BEI) gene which, via a co-suppression effect, reduces the expression of an endogenous gene encoding a BEI protein compared to expression in the non-transgenic potato plant cell; or
(c) a nucleic acid sequence comprising at least 500 nucleotides of a branching enzyme I (BEI) gene that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEI protein compared to expression in the non-transgenic potato plant cell; and
(2) a third foreign nucleic acid molecule comprising
(a) a nucleic acid sequence comprising at least 500 nucleotides of a branching enzyme II (BEII) gene operably linked to a promoter in an antisense orientation leading to reduced expression of an endogenous gene encoding a BEII protein compared to expression in the non-transgenic potato plant cell;
(b) a nucleic acid sequence comprising at least 500 nucleotides of a branching enzyme II (BEII) gene which, via a co-suppression effect, reduces the expression of an endogenous gene encoding a BEII protein compared to expression in the non-transgenic potato plant cell; or
(c) a nucleic acid sequence comprising at least 500 nucleotides of a branching enzyme II (BEII) gene that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEII protein compared to expression in the non-transgenic potato plant cell.
- The starch of claim 1, wherein the phosphate content is reduced by at least 80% in comparison to starch from corresponding wild type potato plant cells.
- The starch of claim 1, wherein the potato plant cells are from the Desiree variety.
- The starch of claim 1, wherein said starch has a phosphate content in the C-6 position of the glucose monomers of up to 7 nmol C6-P mg−1 starch.
- The starch of claim 4, wherein said phosphate content in the C-6 position of the glucose monomers of up to 4 nmol C6-P mg−1 starch.
- The starch of claim 1, wherein said starch has a modified distribution of the side chains.
- The starch of claim 1, wherein said starch has an increased gel strength in comparison to starch from corresponding wild type potato plant cells.
- The starch of claim 7, wherein the gel strength is increased by at least 1000% in comparison to starch from corresponding wild type potato plant cells.
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A method for producing an isolated starch comprising, extracting starch from
(a) a transgenic potato plant cell comprising
(1) a first foreign nucleic acid molecule comprising
(i) a nucleic acid sequence comprising at least 500 nucleotides of a starch phosphorylating (R1) gene operably linked to a promoter in an antisense orientation leading to reduced expression of an endogenous gene encoding a R1 protein compared to expression in the non-transgenic potato plant cell;
(ii) a nucleic acid sequence comprising at least 500 nucleotides of a starch phosphorylating (R1) gene which, via a co-suppression effect, reduces the expression of an endogenous gene encoding a R1 protein compared to expression in the non-transgenic potato plant cell; or
(iii) a nucleic acid sequence comprising at least 500 nucleotides of a starch phosphorylating (R1) gene that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a R1 protein compared to expression in the non-transgenic potato plant cell;
(2) a second foreign nucleic acid molecule comprising
(i) a nucleic acid sequence comprising at least 500 nucleotides of a branching enzyme I (BEI) gene operably linked to a promoter in an antisense orientation leading to reduced expression of an endogenous gene encoding a BEI protein compared to expression in the non-transgenic potato plant cell;
(ii) a nucleic acid sequence comprising at least 500 nucleotides of a branching enzyme I (BEI) gene which, via a co-suppression effect, reduces the expression of an endogenous gene encoding a BEI protein compared to expression in the non-transgenic potato plant cell; or
(iii) a nucleic acid sequence comprising at least 500 nucleotides of a branching enzyme I (BEI) gene that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEI protein compared to expression in the non-transgenic potato plant cell; and
(3) a third foreign nucleic acid molecule comprising
(i) a nucleic acid sequence comprising at least 500 nucleotides of a branching enzyme II (BEII) gene operably linked to a promoter in an antisense orientation leading to reduced expression of an endogenous gene encoding a BEII protein compared to expression in the non-transgenic potato plant cell;
(ii) a nucleic acid sequence comprising at least 500 nucleotides of a branching enzyme II (BEII) gene which, via a co-suppression effect, reduces the expression of an endogenous gene encoding a BEII protein compared to expression in the non-transgenic potato plant cell; or
(iii) a nucleic acid sequence comprising at least 500 nucleotides of a branching enzyme II (BEII) gene that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEII protein compared to expression in the non-transgenic potato plant cell;
(b) a potato plant comprising said transgenic potato plant cell; or
(c) propagation material comprising said transgenic potato plant cell.
- The method of claim 9, comprising extracting starch from said transgenic potato plant cell.
- The method of claim 9, comprising extracting starch from said potato plant.
- The method of claim 9, comprising extracting starch from said propagation material.
- Starch obtained from any of one claims 9-12, wherein the starch has an amylose content of at least 75% and a phosphate content which is reduced by at least 50% in comparison from starch from corresponding wild type plants which are not genetically modified.
Owners (US)
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Basf Agricultural Solutions Seed Us Llc
(Aug 01 2018)
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Bayer Intellectual Property Gmbh
(Mar 25 2015)
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Bayer Cropscience Gmbh
(May 18 2007)
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Bayer Cropscience Ag
(Apr 13 2007)
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Applicants
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Bayer Cropscience Ag
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Inventors
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Uwer Ursula
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Frohberg Claus
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Pilling Jens
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Landschuetze Volker
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CPC Classifications
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C12N9/1051
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C07K14/415
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C12N15/8245
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IPC Classifications
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A01H5/00
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C12N15/82
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C07H21/04
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C07K14/415
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C08B30/00
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C08B30/12
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C12N5/10
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C12N9/10
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C12N15/00
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C12N15/09
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C12N15/11
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C12P19/04
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US Classifications
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800/284
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800/278
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800/285
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800/286
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435/320.1
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435/468
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536/23.1
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536/23.6
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536/24.5
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127/32
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Document Preview
- Publication: Nov 30, 2010
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Application:
Sep 25, 2006
US 52709906 A
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Priority:
Sep 25, 2006
US 52709906 A
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Priority:
Jun 11, 2002
US 17100802 A
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Priority:
Jun 12, 2001
DE 10128363 A