Functional Genomics Using Crispr-cas Systems, Compositions, Methods, Screens And Applications Thereof

Abstract

The present invention generally relates to libraries, kits, methods, applications and screens used in functional genomics that focus on gene function in a cell and that may use vector systems and other aspects related to Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas systems and components thereof. The present invention also relates to rules for making potent single guide RNAs (sgRNAs) for use in CRISPR-Cas systems. Provided are genomic libraries and genome wide libraries, kits, methods of knocking out in parallel every gene in the genome, methods of selecting individual cell knock outs that survive under a selective pressure, methods of identifying the genetic basis of one or more medical symptoms exhibited by a patient, and methods for designing a genome-scale sgRNA library.


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Document History
  • Publication: May 7, 2015
  • Application: Oct 28, 2014
    WO US 2014/0062558 W
  • Priority: Oct 27, 2014
    US US 201462069243 P
  • Priority: Dec 9, 2013
    US US 201361963643 P
  • Priority: Oct 28, 2013
    US US 201361961980 P

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