{"search_session":{},"preferences":{"l":"es","queryLanguage":"es"},"patentId":"007-069-752-827-124","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"007-069-752-827-124"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":12056,"type":"PATENT","title":"Wake Forest University Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":2514,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8584,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately:Wake Forest Univ*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2294
Search Applicants and Owners separately:Wake Forest Univ*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2294
providing urine stem cells isolated from a urine sample; and then\n
differentiating said urine stem cells, wherein said differentiating is osteogenic, chondrogenic, adipogenic, endothelial, neurogenic or myogenic;\n
to produce said culture of differentiated cells."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said differentiating is osteogenic, and wherein said culture of differentiated cells comprises mineralized bone, calcium deposition, osteocalcin, Runx2, alkaline phosphatase, or a combination thereof."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said differentiating is adipogenic, and wherein said culture of differentiated cells express the transcription factor PPARγ, lipoprotein lipase (LpL), or a combination thereof."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said differentiating is chondrogenic, and wherein said culture of differentiated cells express Sox9, collagen II, aggrecan, or a combination thereof."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein differentiating is myogenic, and wherein said culture of differentiated cells express actin, desmin, calponin, myosin, or a combination thereof."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein differentiating is endothelial, and wherein said culture of differentiated cells express vWF, CD 31, or a combination thereof."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein differentiating is neurogenic, and wherein said culture of differentiated cells express nestin."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said urine stem cells are human or mammalian cells."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["An isolated differentiated cell produced by a method of claim 1."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["An isolated urine stem cell, wherein said cell is c-kit positive, and wherein said cell can differentiate into two or more lineages selected from the group consisting of: bone, cartilage, fat, endothelium, nerve and muscle."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The cell of claim 10, wherein said cell is positive for a marker selected from: CD133, SSEA-A, CD90, CD73, CD105, pericyte CD146 (MCAM), NG2, PDGF-Reeeptorβ (PDGF-Rβ), and combinations thereof, and wherein said cell is negative for a marker selected from CD31, CD34, CD45, and combinations thereof."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The cell of claim 10, wherein said cell expresses telomerase."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The cell of claim 10, wherein said cell has undergone hypoxia preconditioning by culturing in a 1-2% O2 environment."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["A method of providing a seeded tissue scaffold, said method comprising the steps of:\n
providing the isolated urine stem cell of claim 10;\n
differentiating said urine stem cell, wherein said differentiating is osteogenic, chondrogenic, adipogenic, endothelial, neurogenic or myogenic, to produce differentiated cells; and\n
seeding said differentiated cells onto a biocompatible tissue scaffold."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 14, wherein said tissue scaffold comprises a collagen matrix."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 14, wherein said tissue scaffold comprises a synthetic polymer."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 14, wherein said tissue scaffold comprises a synthetic polymer selected from the group consisting of: polyglycolic acid (PGA), polylactic acid (PLA), and polylactic-co-glycolic acid (PLGA)."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 14, wherein said tissue scaffold comprises a cellulose polymer."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["A seeded tissue scaffold comprising a biocompatible tissue scaffold and urine stem cells."],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["The seeded tissue scaffold of claim 19, wherein said biodegradable tissue scaffold comprises a collagen matrix."],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["The seeded tissue scaffold of claim 19, wherein said biodegradable tissue scaffold comprises a synthetic polymer."],"number":21,"annotation":false,"title":false,"claim":true},{"lines":["The seeded tissue scaffold of claim 19, wherein said biodegradable tissue scaffold comprises a synthetic polymer selected from the group consisting of: polyglycolic acid (PGA), polylactic acid (PLA) and polylactic-co-glycolic acid (PLGA)."],"number":22,"annotation":false,"title":false,"claim":true},{"lines":["The seeded tissue scaffold of claim 19, wherein said biodegradable tissue scaffold comprises a cellulose polymer."],"number":23,"annotation":false,"title":false,"claim":true},{"lines":["A method of treating a subject in need thereof, said method comprising the steps of:\n
providing a tissue substrate, wherein said substrate comprises urine stem cells;\n
differentiating said urine stem cells, wherein said differentiating is osteogenic, chondrogenic, adipogenic, endothelial, neurogenic or myogenic, to produce differentiated cells;\n
seeding said differentiated cells onto a biocompatible substrate; and\n
transplanting said substrate into said patient."],"number":24,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 24, wherein said substrate comprises a collagen matrix."],"number":25,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 24, wherein said substrate comprises a synthetic polymer."],"number":26,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 24, wherein said substrate comprises a synthetic polymer selected from the group consisting of: polyglycolic acid (PGA), polylactic acid (PLA) and polylactic-co-glycolic acid (PLGA)."],"number":27,"annotation":false,"title":false,"claim":true},{"lines":["Cultured smooth muscle tissue produced by the process of claim 5."],"number":28,"annotation":false,"title":false,"claim":true},{"lines":["The cultured muscle tissue of claim 28, wherein said tissue is characterized by a contractile response to calcium ionophore in vitro."],"number":29,"annotation":false,"title":false,"claim":true},{"lines":["The cultured muscle tissue of claim 28, wherein said tissue is suturable."],"number":30,"annotation":false,"title":false,"claim":true},{"lines":["The cultured muscle tissue of claim 28, wherein said tissue contracts upon administration of a contraction agonist four weeks after of in vivo implantation."],"number":31,"annotation":false,"title":false,"claim":true},{"lines":["A method of treating stress urinary incontinence (UI) or vesicoureteral reflux (VCR) in a subject in need thereof, comprising:\n
administering urine stem cells to said subject in a treatment effective amount."],"number":32,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 32, wherein said administering is carried out by subcutaneous administration."],"number":33,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 32, wherein said urine stem cells are tranfected with a nucleic acid encoding VEFG."],"number":34,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 32, further comprising administering endothelial cells."],"number":35,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 32, wherein said cells are provided in a pharmaceutically acceptable carrier."],"number":36,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 36, wherein said carrier comprises a collagen gel, a hydrogel, a temperature sensitive gel or a hyaluronic acid gel."],"number":37,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 32, wherein said administering is carried out by endoscopic injection."],"number":38,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 32, wherein said subject is in need of treatment for stress urinary incontinence (SUI)."],"number":39,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}