{"search_session":{},"preferences":{"l":"ar","queryLanguage":"ar"},"patentId":"005-693-668-546-81X","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"005-693-668-546-81X"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11618,"type":"PATENT","title":"Université libre de Bruxelles (ULB) - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":2893,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8293,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: univ* AND bruxe*
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Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2688
Search Applicants and Owners separately:univ* AND Montpellier. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1289
Search Applicants and Owners separately: univ* AND bruxe*
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Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2688
Search Applicants and Owners separately:univ* AND Montpellier. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1289
providing cells of a recombinant microbial cell population, which comprise a gene coding for a gene product of interest, with a gene coding for a cytotoxin polypeptide of a proteic killer gene system, wherein said gene coding for said cytotoxin polypeptide is operably linked to a regulatable, regulatory DNA sequence comprising a promoter that regulates the expression of said cytotoxin polypeptide at the transcriptional level in response to the presence or absence of a compound in the medium, such that said cytotoxin is expressed."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1, wherein the cells of said cell population are further provided with a gene coding for an antidote polypeptide of said proteic killer gene system, wherein said antidote polypeptide binds to said cytotoxin polypeptide, thereby resulting in at least a partial counteraction of the toxic effect of said cytotoxin polypeptide."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 2, wherein the gene coding for said antidote polypeptide that binds to said cytotoxin polypeptide of said proteic killer gene system is operably linked to a regulatable, regulatory DNA sequence such that said gene coding for said antidote polypeptide is suppressed under conditions whereby said gene coding for said cytotoxin polypeptide is expressed."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 2, wherein the expression of said genes coding for said cytotoxin and/or antidote polypeptide of said proteic killer gene system is stochastically regulated."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 4, wherein said stochastical regulation is effected by operably linking said genes coding for said cytotoxin and/or antidote polypeptide of said proteic killer gene system to a regulatory sequence that comprises an invertible promoter."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 4, wherein said stochastical regulation is effected by flanking at least part of said regulatory sequence by repeat sequences such that at least part of said regulatory sequence is then recombinationally excised."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1, wherein at least one of said genes of said proteic killer gene system is present on the chromosome of said cell."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1, wherein at least one of said genes of said proteic killer gene system is present on an extrachromosomal replicon."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1, wherein said cytotoxin polypeptide inhibits translation."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1, wherein said genes of said proteic killer gene system are derived from Gram-negative bacteria, Gram-positive bacteria or from a species belonging to the Archae."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1, wherein said Gram-negative bacteria are selected from the group consisting of Enterobacteriaceae spp., Hemophilus spp., Vibrionaceae spp., Pseudomonadaceae spp., Helicobacter spp. and Synechosystis spp."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1, wherein said Gram-positive bacteria are selected from the group consisting of lactic acid bacterial spp., Bacillaceae spp. and Mycobacterium spp."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 12, wherein said Gram-positive bacterium is Bacillus thuringiensis. "],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1, wherein said gene coding for said cytotoxin polypeptide of said proteic killer gene system, when expressed, results in the formation of a cytotoxin polypeptide selected from the group consisting of an E. coli K-12 relE polypeptide, an E. coli plasmid P307 relE polypeptide, a plasmid F CcdB polypeptide, a plasmid R1 PemK polypeptide, a plasmid RP4 ParE polypeptide, a prophage P1 Doc polypeptide, a Streptococcus pneumoniae cytotoxin polypeptide, an Archeon Methanococcus janashii cytotoxin polypeptide and a functionally equivalent polypeptide which is a derivative of any of the aforementioned polypeptides, the sequence of which has been modified by substitution, deletion or addition of one or more amino acids while the gene product of which has retained at least part of the function of the gene product of the non modified sequence."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1, wherein said promoter is inducible by a chemical compound."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1, wherein said promoter is suppressible by a first kind of chemical compound and inducible by a second kind of chemical compound such that when said first kind of compound is depleted from the medium, the promoter is induced by said second kind of compound."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1, wherein said gene product of interest is an immunologically active gene product."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1, wherein said gene product of interest is one that is effective in degradation of an environmental pollutant."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1, wherein said gene product of interest is a pesticidally active product."],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 19, wherein said gene coding for said pesticidally active gene product is derived from Bacillus thuringiensis. "],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1 further comprising the steps of:\n
providing in the cell of the recombinant microbial cell population at least a gene coding for a cytotoxin polypeptide of a proteic killer gene system, said gene expressible in said cells of said cell population, wherein said gene is operably linked to a regulatory DNA sequence; and\n
cultivating said cell population under conditions whereby said gene coding for said cytotoxin polypeptide is expressed, said expression leading to at least a partial killing of said cell population."],"number":21,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said promoter regulates the expression of said cytotoxin polypeptide in response to the presence of a compound in the medium."],"number":22,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said promoter regulates the expression of said cytotoxin polypeptide in response to the absence of a compound in the medium."],"number":23,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}