{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"005-323-362-405-928","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"005-323-362-405-928"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11720,"type":"PATENT","title":"University of Iowa - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":3930,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8373,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: uni* iowa
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Add to collection. Total patents: 3686
Search Applicants and Owners separately: uni* iowa
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Select all patents in the collection and expand by simple families.
Add to collection. Total patents: 3686
a first strand of RNA having a 5′ end and a 3′ end, wherein the first strand is complementary to at least 15 nucleotides of a targeted gene of interest, and\n
a second strand of RNA having a 5′ end and a 3′ end,\n
wherein\n
(i) the first strand is 18 to 30 nucleotides in length and comprises SEQ ID NO: 49 and the second strand is 18 to 30 nucleotides in length and comprises SEQ ID NO: 50;\n
(ii) the first strand is 20 to 30 nucleotides in length and comprises SEQ ID NO: 51 and the second strand is 20 to 30 nucleotides in length and comprises SEQ ID NO: 52; or\n
(iii) the first strand is 23 to 30 nucleotides in length and comprises SEQ ID NO: 53 and the second strand is 23 to 30 nucleotides in length and comprises SEQ ID NO: 54; and\n
wherein at least 12 nucleotides of the first and second strands are complementary to each other and form a small interfering RNA (siRNA) duplex under physiological conditions, and wherein the siRNA silences only one allele of the targeted gene in the cell."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The cell of claim 1, wherein the first strand and the second strand are operably linked by means of an RNA loop strand to form a hairpin structure comprising a duplex structure and a loop structure."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The cell of claim 2, wherein the loop structure contains from 4 to 10 nucleotides."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["An isolated RNA duplex comprising a first strand of RNA, and a second strand of RNA,\n
wherein at least 12 nucleotides of the first and second strands are complementary to each other; and wherein\n
(i) the first strand is 18 to 30 nucleotides in length and comprises SEQ ID NO: 49 and the second strand is 18 to 30 nucleotides in length and comprises SEQ ID NO: 50;\n
(ii) the first strand is 20 to 30 nucleotides in length and comprises SEQ ID NO: 51 and the second strand is 20 to 30 nucleotides in length and comprises SEQ ID NO: 52; or\n
(iii) the first strand is 23 to 30 nucleotides in length and comprises SEQ ID NO: 53 and the second strand is 23 to 30 nucleotides in length and comprises SEQ ID NO: 54; and\n
wherein the first strand and the second strand are operably linked by means of an RNA loop strand to form a hairpin structure comprising a duplex structure and a loop structure."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The RNA duplex of claim 4, wherein the loop structure contains from 4 to 10 nucleotides."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["An expression cassette comprising a nucleic acid encoding at least one strand of an isolated RNA duplex comprising a first strand of RNA, and a second strand of RNA,\n
wherein at least 12 nucleotides of the first and second strands are complementary to each other; and wherein\n
(i) the first strand is 18 to 30 nucleotides in length and comprises SEQ ID NO: 49 and the second strand is 18 to 30 nucleotides in length and comprises SEQ ID NO: 50;\n
(ii) the first strand is 20 to 30 nucleotides in length and comprises SEQ ID NO: 51 and the second strand is 20 to 30 nucleotides in length and comprises SEQ ID NO: 52; or\n
(iii) the first strand is 23 to 30 nucleotides in length and comprises SEQ ID NO: 53 and the second strand is 23 to 30 nucleotides in length and comprises SEQ ID NO: 54."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["A vector comprising the expression cassette of claim 6."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["A vector comprising two expression cassettes, a first expression cassette comprising a nucleic acid encoding the first strand of the RNA duplex of claim 6 and a second expression cassette comprising a nucleic acid encoding the second strand of the RNA duplex of claim 6."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["A cell comprising the expression cassette of claim 6."],"number":9,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}