{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"187-729-879-266-043","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"187-729-879-266-043","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10806,"type":"PATENT","title":" Technion-Israel Institute of Technology - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":1908,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8271,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: \" Technion Israel Inst Techno\"; \"Techni* Israel Inst* Techno*\"
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 4
Search Applicants and Owners separately: \" Technion Israel Inst Techno\"; \"Techni* Israel Inst* Techno*\"
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 4
(a) isolating foreskin cells from foreskin tissue;\n
(b) culturing said foreskin cells in a culture medium including serum and/or serum replacement thereby preparing said human foreskin fibroblast feeder cell line."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said embryonic stem cells are of human origin."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 2, wherein said foreskin tissue is obtained from an 8-14 day old male individual."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 2, wherein step (a) is effected by:\n
(i) mincing said foreskin tissue;\n
(ii) dissociating said foreskin tissue resultant from step (i) into single cells."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 5, wherein step (ii) is effected via treatment with trypsin."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 2, wherein said serum and/or said serum replacement are provided at a concentration of at least 10%."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 2, wherein said serum is human serum."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 2, wherein said serum and/or said serum replacement are provided at a concentration of 15%."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["A cell culture comprising:\n
(i) embrynoic stem cells; and\n
(ii) human foreskin fibroblast cells capable of maintaining said embryonic stem cells in an undifferentiated state."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The cell culture of claim 10, wherein said embryonic stem cells are of human origin."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The cell culture of claim 10, wherein said human foreskin fibroblast cells are capable of forming a mono-layer in the cell culture."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The cell culture of claim 10, wherein said cell culture includes a culture medium including serum replacement and/or human serum."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The cell culture of claim 13, wherein said serum replacement and/or said human serum are provided at a concentration of at least 10%."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The cell culture of claim 13, wherein said serum replacement and/or said human serum are provided at a concentration of 15%."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The cell culture of claim 10, wherein said human foreskin fibroblast cells of said cell culture maintain said embryonic stem cells in an undifferentiated, proliferative state through at least 87 passages."],"number":16,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}