{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"171-704-257-915-211","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"171-704-257-915-211","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8838,"type":"PATENT","title":"Univ of Illinois Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":10245,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8209,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately = \"Univ Illinois\", \"Illinois Univ\", \"University of Illinois\", \"Illinois University\", \"University Illinois NOT Northern NOT southern NOT State\".
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Total patents: 9630
Search applicants and owners= \"Johns Hopkins Univ\", \"Univ Johns Hopkins\"
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Total patents: 12941
Search Applicants and Owners separately = \"Univ Illinois\", \"Illinois Univ\", \"University of Illinois\", \"Illinois University\", \"University Illinois NOT Northern NOT southern NOT State\".
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Total patents: 9630
Search applicants and owners= \"Johns Hopkins Univ\", \"Univ Johns Hopkins\"
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Total patents: 12941
providing a membrane having one or more nanopores, wherein said membrane separates a first compartment from a second compartment and the nanopore is in fluid communication with the first and second compartments;\n
providing DNA comprising a heterogeneous mixture of DNA molecules having different methylation parameters to said first compartment;\n
separating said DNA by establishing an electric field across the membrane, and\n
generating a threshold voltage across said nanopore from said electric field to force methylated DNA having the methylation parameter through said nanopore from said first to said second compartment without substantially allowing unwanted DNA not having the methylation parameter to pass to said second compartment to concentrate methylated DNA having the methylation parameter in said second compartment by a factor that is greater than or equal to 1000 the number of DNA not having the methylation parameter in said second compartment."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein said methylation parameter is methylation content, methylation pattern, or methylation content and pattern."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 2 wherein said methylation parameter is methylation content."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 3, wherein said method is capable of separating DNA differing by one methylation site per 100 bp length of DNA."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said DNA comprises at least a portion of a gene."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 5, wherein methylation of said gene is associated with a disease state."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6, wherein said disease state is cancer."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6, wherein said gene is selected from a group consisting of:\n
BRCA1 (breast cancer 1 gene);\n
MS3 (male-sterile gene); and\n
fragments thereof."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said membrane comprises silicon nitride (Si3N4)."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said membrane has a thickness that is selected from a range that is greater than or equal to 10 nm and less than or equal to 50 nm."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said pore diameter is less than or equal to the effective diameter of linear DNA."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said pore diameter is selected from a range that is greater than or equal to 1.5 nm and less than or equal to 3 nm."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12 wherein said pore diameter is selected from a range that is greater than or equal to 1.6 nm and less than or equal to 2.5 nm."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12, wherein said pore has a pore geometry comprising a cone angle, a minimum diameter, a maximum diameter, and a thickness, wherein said pore geometry is selected to generate a force on a DNA molecule within the pore that is sufficient to stretch the DNA."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said electric field is selected to generate said threshold voltage that is capable of forcing DNA having a methylation content that is equal to or greater than a user-selected methylation content."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said threshold voltage is selected to measure said methylation parameter."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 16, wherein said methylation parameter is the number of methylated sites on said DNA."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said DNA is double stranded and has a sequence length that is selected from a range that is greater than or equal to 50 base pairs."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 18, wherein said sequence length is selected from a range that is greater than or equal to 50 base pairs and less than or equal to 1000 base pairs."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, further comprising:\n
analyzing at least a portion of DNA from said second compartment."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 20, wherein said analyzing step comprises DNA sequencing by polymerase chain reaction."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, further comprising:\n
sequentially changing said electric field over a plurality of said separating steps to obtain a plurality of individually separated DNA, wherein each separated DNA has a methylation pattern or level different from any other separated DNA."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 22, wherein said sequentially changing step is used to separate said provided DNA based on methylation level, wherein said DNA comprises a mixture of unmethylated, hemimethylated and methylated DNA."],"number":23,"annotation":false,"claim":true,"title":false},{"lines":["A method of detecting a DNA methylation content or methylation pattern from DNA obtained from a subject, said method comprising:\n
obtaining a DNA sample from said subject, said DNA sample comprising a heterogeneous mixture of DNA molecules having different methylation content or methylation pattern;\n
providing a membrane having one or more nanopores, wherein said membrane separates a first compartment from a second compartment and the nanopore is in fluid communication with the first and second compartments;\n
providing said DNA sample to said first compartment;\n
establishing an electric field across the membrane, wherein said electric field generates a threshold voltage across said nanopore that is capable of forcing DNA having said methylation content or methylation pattern, without substantially passing DNA not having said methylation content or methylation pattern, through said nanopore from said first to said second compartment to concentrate DNA having said methylation content or methylation pattern in said second compartment by a factor that is greater than or equal to 1000 compared to other DNA in said second compartment not having said methylation content or said methylation parameter; and\n
detecting said methylation level or pattern by the passage of said methylated content or pattern through said nanopore or by detecting the presence of said methylated content or pattern of DNA in said second compartment."],"number":24,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 24, wherein said electric field is constant over time."],"number":25,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 24, wherein said electric field varies over time."],"number":26,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 24 further comprising determining the methylation content of said DNA by selecting said electric field to generate a force capable of translocating said DNA having a methylation content or greater than a user-selected methylation content through said nanopore, and wherein said force is insufficient to translocated a substantial number of DNA having methylation content lower than said user-selected value."],"number":27,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 24, wherein said DNA comprises at least a portion of a gene, wherein said gene methylation level or methylation pattern is associated with a disease state."],"number":28,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 28, wherein said disease state is cancer."],"number":29,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 24, wherein said subject is a human."],"number":30,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}