{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"164-353-644-366-413","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"164-353-644-366-413","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11813,"type":"PATENT","title":"University of Aberdeen Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":27926,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8443,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: univ* aberdeen. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1658
Search Applicants and Owners separately: univ* aberdeen. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1658
a DNA encoding dihydrodipicolinate synthase which is desensitized to feedback inhibition by L-lysine; and
a DNA encoding a mutant LysE protein that promotes export of L-lysine to the outside of a bacterial cell when it is introduced into a methanol-assimilating bacterium, wherein said bacterium is modified to enhance the intracellular activities of diaminopimelate dehydrogenase, diaminopimelate decarboxylase, dihydrodipicolinate reductase, and aspartate-semialdehyde dehydrogenase."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The methanol-assimilating bacterium according to claim 1, further comprising:
a DNA encoding an aspartokinase which is desensitized to feedback inhibition by L-lysine."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The methanol-assimilating bacterium according to claim 1 or 2, wherein said bacterium is introduced with DNAs encoding the enzymes or the mutant LysE protein into chromosomal DNA and/or transformed with a plasmid(s) carrying DNAs encoding the enzymes or the mutant LysE protein."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The methanol-assimilating bacterium according to claim 3, wherein the DNA encoding dihydrodipicolinate synthase, the DNA encoding dihydrodipicolinate reductase, the DNA encoding aspartate-semialdehyde dehydrogenase, and the DNA encoding aspartokinase are derived from a bacterium belonging to the genus Escherichia, the DNA encoding diaminopimelate decarboxylase is derived from a bacterium belonging to the genus Methylophilus, and the DNA encoding diaminopimelate dehydrogenase and the DNA encoding mutant LysE are derived from a bacterium belonging to the genus Brevibacterium."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The methanol-assimilating bacterium according to any one of claims 1 to 4, wherein said bacterium belongs to the genus Methylophilus."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The methanol-assimilating bacterium according to any one of claims 1 to 5, wherein said bacterium is Methylophilus methylotrophus AJ110196 strain (FERM BP-10434)."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["A method of producing L-lysine, comprising culturing the methanol-assimilating bacterium according to any one of claims 1 to 6 in a medium to produce and accumulate L-lysine in the medium or bacterial cells; and collecting L-lysine from the medium or bacterial cells."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of producing L-lysine according to claim 7, wherein the main carbon source of the medium is methanol."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["Methylophilus methylotrophus AJ110196 strain (FERM BP-10434)."],"number":9,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}