{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"156-581-040-685-717","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"156-581-040-685-717"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8903,"type":"PATENT","title":"Johns Hopkins Univ Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":17938,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8218,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search applicants and owners= \"Johns Hopkins Univ\", \"Univ Johns Hopkins\"
Select more for logical variants
Add to collection
Total patents: 12941
Search applicants and owners= \"Johns Hopkins Univ\", \"Univ Johns Hopkins\"
Select more for logical variants
Add to collection
Total patents: 12941
plating a single-cell suspension of hESCs or hiPSCs onto a surface coated with suitable materials selected from the group consisting of type I collagen, fibronectin, and type IV collagen;\n
adding culture medium;\n
culturing the cells for several days;\n
adding Vascular Endothelial Growth Factor (VEGF) and a transforming growth factor-beta (TGF-β) inhibitor to the culture medium;\n
culturing the cells for several days; and\n
harvesting the resulting EVCs,\n
wherein said EVCs express CD73 and one or more of CD105 and CD146."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method for differentiating of claim 1, wherein the TGF βinhibitor is SB431542."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method for differentiating of claim 1, wherein the concentration of VEGF is between about 1 and 50 ng/ml."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method for differentiating of claim 1, wherein the suitable material is type IV collagen."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method for differentiating of claim 1, wherein the media used for culturing are supplemented with serum."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method for differentiating of claim 5, wherein the media are supplemented with 10% serum."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method for differentiating of claim 1, wherein prior to the step of adding VEGF the cells are harvested from the surface and then plated onto a surface coated with the suitable materials with VEGF in the culture medium."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method for differentiating of claim 1, further comprising a step of embedding the harvested resulting EVC cells in a matrix following the step of harvesting."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method for differentiating of claim 8, wherein after embedding the harvested resulting EVC cells in a matrix, the cells comprise a population that can self-organize into vascular networks."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method for differentiating of claim 9, wherein the matrix is a hydrophilic matrix."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method for differentiating of claim 10, wherein the hydrophilic matrix is a hydrogel selected from the group consisting of hyaluronic acid (HA) hydrogel and collagen hydrogel."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method for differentiating of claim 8, wherein the matrix is a hydrophilic matrix."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The method for differentiating of claim 12, wherein the hydrophilic matrix is a hydrogel selected from the group consisting of hyaluronic acid (HA) hydrogel and collagen hydrogel."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method for differentiating of claim 1, further comprising the step of sub-culturing the EVCs in a medium containing serum on a tissue-culture treated surface and removing unattached cells after 4 hours."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The method for differentiating of claim 14, wherein the medium for sub-culturing EVCs also contains Angiopoietin 1 or TGFb-1."],"number":15,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}