{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"145-979-161-582-280","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"145-979-161-582-280","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11616,"type":"PATENT","title":"Case Western Reserve University - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":4001,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8291,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: weste* reser* AND Univ*
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2737
Search Applicants and Owners separately: weste* reser* AND Univ*
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2737
a self-assembled, scaffold-free, high-density cell aggregate, the cell aggregate including a plurality of cells and a plurality of biocompatible and degradable nanoparticles and/or microparticles that are incorporated within the cell aggregate, the nanoparticles and/or microparticles acting as a bulking agent within the cell aggregate to increase the cell aggregate size and/or thickness and improve the mechanical properties of the cell aggregate allowing the cell aggregate to be readily manipulated and formed into tissue constructs with defined architectures."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 1, the nanoparticles and/or microparticles having a diameter of about 1 nm to about 200 μm."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 2, the size, shape, and/or composition of the nanoparticles and/or microparticles provided in the tissue construct being varied to control, alter and/or adjust the function of the cells in the construct and/or mechanical properties of the tissue construct."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 1, the nanoparticles and/or microparticles comprising a biocompatible and/or degradable polymer."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 4, the degradable and/or biocompatible polymer including a hydrogel that comprises macromers, the macromers being cross-linked to vary the bioactivity and/or mechanical properties and/or degradation profile of the nanoparticles and/or microparticles."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 4, wherein the degradable and/or biocompatible polymer is gelatin and the gelatin is cross-linked with genipin."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 1, the nanoparticles and/or microparticles including at least one bioactive agent that is differentially and/or controllably released by the nanoparticles and/or microparticles."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 7, the bioactive agent(s) being incorporated in and/or physically associated with the nanoparticles and/or microparticles and spatially presented in specific locations within the aggregate and/or temporally released with a defined release profile from the nanoparticles and/or microparticles."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 1, the cell aggregate comprising undifferentiated and/or substantially differentiated progenitor cells, a plurality of nanoparticles and/or microparticles, and/or a self-secreted extracellular matrix that promotes or affects specific function or functions of the cells and/or promotes their adherence to the nanoparticles and/or microparticles."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 1, the cells being at least about 30%, by volume of the cell aggregate based on the total volume of the cell aggregate."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 10, the progenitor cells being isolated from at least one of tendon/ligament tissue, bone morrow, adipose tissue or dental pulp."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 1, the cell aggregate being a self-assembled, scaffold-free, high density cell sheet."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 12, comprising a heterogenous cell aggregate that includes defined regions or portions of differing or similar cell aggregate materials, at least one of the differing regions or portions of the heterogenous cell aggregate being provided or formed with or without nanoparticles and/or microparticles and having similar or different properties to vary the properties of the tissue construct for particular tissue engineering application."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 13, the heterogenous cell aggregate comprising layers of cell sheets comprising different cells, nanoparticles and/or microparticles, and/or mechanical properties to vary the properties of the tissue construct for particular tissue engineering applications."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 1, the cell aggregate being modified to form an acellular aggregate that is free of cells."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["A tissue construct comprising:\n
a self-assembled, scaffold-free, high-density cell aggregate, the cell aggregate including a plurality of undifferentiated and/or substantially differentiated progenitor cells, a plurality of degradable and biocompatible nanoparticles and/or microparticles, and/or a self-secreted extracellular matrix of the undifferentiated and/or substantially differentiated progenitor cells, the nanoparticles and/or microparticles acting as a bulking agent within the cell sheet to increase the cell sheet thickness and improve the mechanical properties of the cell sheet allowing the cell sheet to be readily manipulated and formed into tissue constructs with defined architectures, wherein the size, shape, and/or composition of the nanoparticles and/or microparticles provided in the tissue construct being varied to control, alter and/or adjust the function of the cells in the construct and/or mechanical properties of the tissue construct."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 16, the nanoparticles and/or microparticles having a diameter of about 1 nm to about 200 μm."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 16, the nanoparticles and/or microparticles comprising a biocompatible and/or degradable polymer."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 18, the degradable and/or biocompatible polymer including a hydrogel that comprises macromers, the macromers being cross-linked to vary the bioactivity and/or mechanical properties and/or degradation profile of the nanoparticles and/or microparticles."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 16, the nanoparticles and/or microparticles including at least one bioactive agent that is differentially and/or controllably released by the nanoparticles and/or microparticles."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 20, the bioactive agent being incorporated in and/or physically associated with the nanoparticles and/or microparticles and spatially presented in specific locations within the aggregate and/or temporally released with a defined release profile from the nanoparticles and/or microparticles."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 16, comprising a heterogenous cell aggregate that includes defined regions or portions of differing or similar cell aggregate materials, at least one of the differing regions or portions of the heterogenous cell aggregate being provided or formed with or without nanoparticles and/or microparticles and having similar or different properties to vary the properties of the tissue construct for particular tissue engineering application."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 22, the heterogenous cell aggregate comprising layers of cell sheets comprising different cells, nanoparticles and/or microparticles, and/or mechanical properties to vary the properties of the tissue construct for particular tissue engineering applications."],"number":23,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 16, have shape that is defined by or substantially similar to the tissue defect or region being treated."],"number":24,"annotation":false,"claim":true,"title":false},{"lines":["The tissue construct of claim 1, the cell aggregate being modified to form an acellular aggregate that is free of cells."],"number":25,"annotation":false,"claim":true,"title":false},{"lines":["A method of forming a cell aggregate, the method comprising:\n
isolating undifferentiated and/or substantially differentiated progenitor cells;\n
expanding the undifferentiated and/or substantially differentiated progenitor cells;\n
combining the undifferentiated and/or substantially differentiated progenitor cells with a plurality of the nanoparticles and/or microparticles so that the nanoparticles and/or microparticles are dispersed and suspended with the undifferentiated and/or substantially differentiated progenitor cells in a culture medium; and culturing the suspension of nanoparticles and/or microparticles and undifferentiated and/or substantially differentiated progenitor cells so that an cell aggregate is formed that can be readily manipulated and formed into tissue constructs with defined architectures."],"number":26,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 26, further comprising mechanically stimulation the cell aggregate and/or tissue construct to enhance cell differentiation and tissue formation of the tissue construct."],"number":27,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}