{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"143-829-589-078-96X","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"143-829-589-078-96X","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10771,"type":"PATENT","title":"University of Southern California - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":8387,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8241,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately:univ* AND Liege. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1416
Search Applicants and Owners separately:univ* AND Liege. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1416
(a) contacting a genomic sample of DNA with a modifying agent that modifies unmethylated cytosine to produce a converted nucleic acid;\n
(b) amplifying the converted nucleic acid by means of oligonucleotide primers in the presence of one or a plurality of specific oligonucleotide probes, wherein one or a plurality of the oligonucleotide primers or the specific probe(s) are capable of distinguishing between unmethylated and methylated nucleic acid, with the proviso that at least one oligonucleotide probe is a CpG-specific probe capable of distinguishing between unmethylated and methylated nucleic acid; and\n
(c) detecting, in real-time during the amplification, the methylated nucleic acid based on amplification-mediated probe displacement."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the amplifying step is a polymerase chain reaction (PCR)."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the modifying agent is bisulfite."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the converted nucleic acid contains uracil in place of unmethylated cytosine residues present in the unmodified nucleic acid-containing sample."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the probe further comprises one or a plurality of fluorescence label moieties."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 5 wherein the amplification and detection step comprises fluorescence-based quantitative PCR."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 5, wherein the probe is a FRET probe, or a dual-label probe comprising a fluorescence-reporter moiety and fluorescence-quencher moiety."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 7, wherein the FRET probe is one component of a real-time PCR hybridization probe pair."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 7, wherein the probe is a nuclease cleavable dual-label probe."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein at least one of the primers comprises a CpG-speciflc probe."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein detecting based on amplification-mediated probe displacement, comprises detecting amplification-mediated change of probe fluorescence."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["A method for detecting a methylated CpG-containing nucleic acid comprising:\n
(a) contacting a nucleic acid-containing sample with a modifying agent that modifies unmethylated cytosine to produce a converted nucleic acid;\n
(b) amplifying the converted nucleic acid in the sample by means of oligonucleotide primers in the presence of a CpG-specific oligonucleotide probe, wherein the CpG-specific probe, but not the primers, distinguishes between modified unmethylated and methylated nucleic acid; and\n
(c) detecting, in real-time during the amplification, the methylated nucleic acid based on amplification-mediated probe displacement."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12 wherein the amplifying step comprises a polymerase chain reaction (PCR)."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12 wherein the modifying agent comprises bisulfite."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12 wherein the converted nucleic acid contains uracil in place of unmethylated cytosine residues present in the unmodified nucleic acid-containing sample."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12 wherein the probe further comprises one or a plurality of fluorescence label moieties."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 16 wherein the amplification and detection step comprises fluorescence-based quantitative PCR."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 16, wherein the probe is a FRET probe, or a dual-label probe comprising a fluorescence-reporter moiety and fluorescence-quencher moiety."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 18, wherein the FRET probe is one component of a real-time PCR hybridization probe pair."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 18, wherein the probe is a nuclease cleavable dual-label probe."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12, wherein at least one of the primers comprises a CpG-specific probe."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12, wherein detecting based on amplification-mediated probe displacement, comprises detecting amplification-mediated change of probe fluorescence."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12 wherein methylation amounts in the nucleic acid sample are quantitatively determined based on reference to a control reaction for amount of input nucleic acid."],"number":23,"annotation":false,"claim":true,"title":false},{"lines":["A method for detecting a methylated CpG-containing nucleic acid comprising:\n
(a) contacting a nucleic acid-containing sample with a modifying agent that modifies unmethylated cytosine to produce a converted nucleic acid;\n
(b) amplifying the converted nucleic acid in the sample by means of oligonucleotide primers in the presence of a CpG-specific oligonucleotide probe, wherein both the primers and the CpG-specific probe distinguish between modified unmethylated and methylated nucleic acid; and\n
(c) detecting, in real-time during the amplification, the methylated nucleic acid based on amplification-mediated probe displacement."],"number":24,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 24 wherein the amplifying step comprises a polymerase chain reaction (PCR)."],"number":25,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 24 wherein the modifying agent is bisulfite."],"number":26,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 24 wherein the converted nucleic acid contains uracil in place of unmethylated cytosine residues present in the unmodified nucleic acid-containing sample."],"number":27,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 24 wherein the probe further comprises one or a plurality of fluorescence label moieties."],"number":28,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 28 wherein the amplification and detection step comprises fluorescence-based quantitative PCR."],"number":29,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 28, wherein the probe is a FRET probe, or a dual-label probe comprising a fluorescence-reporter moiety and fluorescence-quencher moiety."],"number":30,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 30, wherein the FRET probe is one component of a real-time PCR hybridization probe pair."],"number":31,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 30, wherein the probe is a nuclease cleavable dual-label probe."],"number":32,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 24, wherein at least one of the primers comprises a CpG-specific probe."],"number":33,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 24, wherein detecting based on amplification-mediated probe displacement, comprises detecting amplification-mediated change of probe fluorescence."],"number":34,"annotation":false,"claim":true,"title":false},{"lines":["A method for detecting cytosine methylation within a genomic sample of DNA, comprising: obtaining a methylation kit; conducting, using the kit, a real-time methylation assay of the genomic DNA sample; and determining, based on the methylation assay, whether cytosine methylation is present in the DNA sample, and wherein the methylation kit comprises:\n
a modifying agent that modifies unmethylated cytosine to produce a converted nucleic acid;\n
primers for amplification of the converted nucleic acid;\n
primers for the amplification of control unmodified nucleic acid; and\n
a CpG-specific probe the detection of which, during the amplification of the converted nucleic acid, is in real-time based on amplification-mediated probe displacement, wherein the CpG-specific probe distinguishes between modified unmethylated and methylated nucleic acid, and wherein the primers each may or may not distinguish between unmethylated and methylated nucleic acid."],"number":35,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 35, wherein the modifying agent is bisulfite."],"number":36,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 35 wherein the modifying agent converts cytosine residues to uracil residues."],"number":37,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 35, wherein the CpG-speciflc probe, but not the primers for amplification of the converted nucleic acid, distinguishes between modified unmethylated and methylated nucleic acid."],"number":38,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 35, wherein both the CpG-specific probe, and the primers for amplification of the converted nucleic acid, distinguish between modified unmethylated and methylated nucleic acid."],"number":39,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 35, wherein the CpG-specific probe further comprises one or a plurality of fluorescence label moieties."],"number":40,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 35, wherein one of the primers for amplification of the converted nucleic acid comprises the CpG-specific probe."],"number":41,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 40, wherein the CpG-specific probe is a FRET probe, a real-time PCR hybridization probe, or a dual-label probe."],"number":42,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 35, wherein detecting based on amplification-mediated probe displacement, comprises detecting amplification-mediated change of probe fluorescence."],"number":43,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}