{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"137-232-544-627-225","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"137-232-544-627-225"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8837,"type":"PATENT","title":"University of Minnesota Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":8933,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8208,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicant and Owners respectively = \"Univ Minnesota\" , \" Minnesota Univ\", \" University of Minnesota\", \"Minnesota University\", \"Univ Minne*\" , \" Minne* Univ*.
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Total patents: 7910
Search Applicant and Owners respectively = \"Univ Minnesota\" , \" Minnesota Univ\", \" University of Minnesota\", \"Minnesota University\", \"Univ Minne*\" , \" Minne* Univ*.
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Total patents: 7910
a nucleic acid sequence positioned between at least two inverted repeats that can bind to an SB protein, the inverted repeats comprising at least one direct repeat comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO.8, SEQ ID NO:9, and SEQ ID NO:10;
wherein the SB protein comprises an amino acid sequence having at least 80% identity to SEQ ID NO:1, binds to the inverted repeat sequence of at least one of SEQ ID NO:4 and SEQ ID NO:5, and catalyzes the integration of the nucleic acid fragment into DNA in an isolated or cultured vertebrate cell."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The fragment of claim 1 wherein the nucleic acid fragment is part of a plasmid."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The fragment of claim 1 wherein the nucleic acid sequence comprises at least a portion of an open reading frame."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The fragment of claim 1 wherein the nucleic acid sequence comprises at least one expression control region."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The fragment of claim 4 wherein the expression control region is selected from the group consisting of a promoter, an enhancer or a silencer."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The fragment of claim 1 wherein the nucleic acid sequence comprises a promoter operably linked to at least a portion of an open reading frame."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The fragment of claim 1 wherein the vertebrate cell is fish cell."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The fragment of claim 1 wherein the vertebrate cell is a mammalian cell."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The fragment of claim 8 wherein the vertebrate cell is a mouse cell or a human cell."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The fragment of claim 1 wherein the DNA into which the fragment is capable of integrating is the cell genome or extrachromosomal DNA."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The fragment of claim 10 wherein the extrachromosomal DNA is selected from the group consisting of an episome and a plasmid."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid fragment of claim 1 wherein at least one of the inverted repeats comprises SEQ ID NO:4 or SEQ ID NO:5."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid fragment of claim 1 wherein the inverted repeats can bind to an SB protein comprising the amino acid sequence of SEQ ID NO:1."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["A gene transfer system to introduce DNA into the DNA of an isolated or cultured vertebrate cell comprising:
a transposase or a nucleic acid encoding a transposase, wherein the transposase is an SB protein; and
a nucleic acid fragment comprising a nucleic acid sequence positioned between at least two inverted repeats that can bind to the SB protein, the inverted repeats comprising at least one direct repeat comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO. 8, SEQ ID NO:9, and SEQ ID NO:10;
wherein the SB protein comprises amino acid sequence having at least 80% identity to SEQ ID NO:1, binds to the inverted repeat sequence of at least one of SEQ ID NO:4 and SEQ ID NO:5, and catalyzes the integration of the nucleic acid fragment into DNA in the vertebrate cell."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 14 wherein the SB protein comprises SEQ ID NO:1."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 14 wherein the transposase is provided to the cell as a protein."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 14 wherein the transposase is provided to the cell as nucleic acid encoding a transposase."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 17 wherein the nucleic acid encoding a transposase is RNA."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 14 wherein the nucleic acid encoding the transposase is integrated into the genome of the cell."],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 14 wherein the nucleic acid fragment is part of a plasmid or a recombinant viral vector."],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 14 wherein the nucleic acid sequence comprises at least a portion of an open reading frame."],"number":21,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 14 wherein the nucleic acid sequence comprises at least a regulatory region of a gene."],"number":22,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 22 wherein the regulatory region is a transcriptional regulatory region."],"number":23,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 22 wherein the regulatory region is selected from the group consisting of a promoter, an enhancer, a silencer, a locus-control region, and a border element."],"number":24,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 14 wherein the nucleic acid sequence comprises a promoter operably linked to at least a portion of an open reading frame."],"number":25,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 14 wherein the vertebrate cell is a fish cell."],"number":26,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 14 wherein the vertebrate cell is a mammalian cell."],"number":27,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 27 wherein the mammalian cell is a mouse cell or a human cell."],"number":28,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 14 wherein the DNA of a cell is the cell genome or extrachromosomal DNA."],"number":29,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 29 wherein the extrachromosomal DNA is selected from the group consisting of an episome and a plasmid."],"number":30,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 14 wherein at least one of the inverted repeats comprises SEQ ID NO:4 or SEQ ID NO:5."],"number":31,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 14 wherein the nucleic acid sequence is obtained from a library of recombinant sequences."],"number":32,"annotation":false,"title":false,"claim":true},{"lines":["The gene transfer system of claim 14 wherein the nucleic acid sequence is introduced into the cell using a method selected from the group consisting of:
particle bombardment;
electroporation;
microinjection;
combining the nucleic acid fragment with lipid-containing vesicles or DNA condensing reagents; and
incorporating the nucleic acid fragment into a viral vector and contacting the viral vector with the cell."],"number":33,"annotation":false,"title":false,"claim":true},{"lines":["A nucleic acid encoding an SB protein, wherein the SB protein comprises an amino acid sequence having at least 80% identity to SEQ ID NO:1, binds to the inverted repeat sequence of at least one of SEQ ID NO:4 and SEQ ID NO:5, and catalyzes the integration of nucleic acid into DNA in an isolated or cultured vertebrate cell."],"number":34,"annotation":false,"title":false,"claim":true},{"lines":["A nucleic acid vector comprising the nucleic acid of claim 34 ."],"number":35,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid vector of claim 35 which is a gene expression vector."],"number":36,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid vector of claim 36 which is a plasmid."],"number":37,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 34 wherein the nucleic acid is a linear nucleic acid fragment."],"number":38,"annotation":false,"title":false,"claim":true},{"lines":["An isolated or cultured vertebrate cell comprising the nucleic acid of claim 34 ."],"number":39,"annotation":false,"title":false,"claim":true},{"lines":["The isolated or cultured vertebrate cell of claim 39 which is a fish cell."],"number":40,"annotation":false,"title":false,"claim":true},{"lines":["The isolated or cultured vertebrate cell of claim 39 which is a mammalian cell."],"number":41,"annotation":false,"title":false,"claim":true},{"lines":["An isolated or cultured vertebrate cell comprising the nucleic acid of claim 34 , wherein the nucleic acid is integrated into the genome of the cell."],"number":42,"annotation":false,"title":false,"claim":true},{"lines":["An SB protein comprising the amino acid sequence of SEQ ID NO:1."],"number":43,"annotation":false,"title":false,"claim":true},{"lines":["A method for introducing nucleic acid into DNA in an isolated or cultured vertebrate cell comprising the step of:
introducing into the cell a nucleic acid fragment comprising a nucleic acid sequence positioned between at least two inverted repeats that can bind to an SB protein, the inverted repeats comprising at least one direct repeat comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO. 8, SEQ ID NO:9, and SEQ ID NO:10; wherein the SB protein comprises an amino acid sequence having at least 80% identity to SEQ ID NO:1, binds to the inverted repeat sequence of at least one of SEQ ID NO:4 and SEQ ID NO:5, and catalyzes the integration of the nucleic acid fragment into DNA in the cell."],"number":44,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 44 further comprising introducing the SB protein into the cell."],"number":45,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 45 wherein the SB protein is introduced to the cell as RNA."],"number":46,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 44 wherein the cell comprises nucleic acid encoding the SB protein."],"number":47,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 47 wherein the nucleic acid encoding the SB protein is integrated into the cell genome."],"number":48,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 47 wherein the SB protein is stably expressed in the cell."],"number":49,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 47 wherein the SB protein is under the control of an inducible promoter."],"number":50,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 44 wherein introducing the nucleic acid fragment into the cell comprises using a method selected from the group consisting of:
microinjection;
electroporation;
combining the nucleic acid fragment with cationic lipid vesicles or DNA condensing reagents; and
incorporating the nucleic acid fragment into a viral vector and contacting the viral vector with the cell."],"number":51,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 51 wherein the viral vector is selected from the group consisting of a retroviral vector, an adenovirus vector or an adeno-associated viral vector."],"number":52,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 44 further comprising introducing a DNA or an RNA encoding the SB protein into the cell."],"number":53,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 44 wherein the nucleic acid sequence encodes a protein."],"number":54,"annotation":false,"title":false,"claim":true},{"lines":["An isolated or cultured vertebrate cell producing the protein of claim 54 ."],"number":55,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 44 wherein the protein is a marker protein."],"number":56,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 44 wherein the inverted repeats can bind to an SB protein comprising the amino acid sequence of SEQ ID NO:1."],"number":57,"annotation":false,"title":false,"claim":true},{"lines":["A protein comprising an amino acid sequence having at least 80% identity to SEQ ID NO:1, wherein the protein binds to the inverted repeat sequence of at least one of SEQ ID NO:4 and SEQ ID NO:5 and catalyzes the integration of nucleic acid into DNA of a vertebrate cell."],"number":58,"annotation":false,"title":false,"claim":true},{"lines":["A protein with transposase activity and comprising an amino acid sequence having at least 80% identity to SEQ ID NO:1, wherein the protein binds to at least one nucleic acid sequence selected from the group consisting of SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9 and SEQ ID NO:10."],"number":59,"annotation":false,"title":false,"claim":true},{"lines":["A nucleic acid encoding an SB protein comprising the amino acid sequence of SEQ ID NO:1."],"number":60,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 60 selected from the group consisting of RNA and DNA."],"number":61,"annotation":false,"title":false,"claim":true},{"lines":["A composition comprising a vertebrate cell comprising a nucleic acid encoding an SB protein, wherein the SB protein comprises an amino acid sequence having at least 80% identity to SEQ ID NO:1, binds to the inverted repeat sequence of at least one of SEQ ID NO:4 and SEQ ID NO:5, and catalyzes the integration of nucleic acid into DNA in the vertebrate cell."],"number":62,"annotation":false,"title":false,"claim":true},{"lines":["The composition of claim 62 wherein the nucleic acid is integrated into the genome of the vertebrate cell."],"number":63,"annotation":false,"title":false,"claim":true},{"lines":["A composition comprising a vertebrate cell comprising an SB protein comprising an amino acid sequence having at least 80% identity to SEQ ID NO:1, wherein the SB protein binds to the inverted repeat sequence of at least one of SEQ ID NO:4 and SEQ ID NO:5 and catalyzes the integration of nucleic acid into DNA in the vertebrate cell."],"number":64,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}