{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"136-084-176-894-347","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"136-084-176-894-347","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11920,"type":"PATENT","title":"Indian Institute of Science Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":3853,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8508,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately:india* AND insti* AND Scien*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 535
Search Applicants and Owners separately:india* AND insti* AND Scien*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 535
b. providing a second sample comprising non malignant tissue from the gingivo-buccal area of the oral cavity of said cancer patient;\n
c. micro-dissecting transformed epithelial cells from frozen sections of the malignant tissue and the non transformed epithelial layer from frozen sections of the non malignant tissue;\n
d. separating proteins of lysates prepared from the micro-dissected transformed and non transformed epithelial cells from (c) by two dimensional gel electrophoresis and staining with silver;\n
e. generating micro-dissected epithelial cell protein profiles from the samples in c) and d);\n
f. comparing the micro-dissected epithelial cell protein profiles generated in step (e) and generating a short list of protein spots commonly expressed in both normal and transformed epithelium;\n
g. punching out the short listed protein spots from a silver stained two dimensional gel of micro-dissected transformed epithelial cells;\n
h. destaining and drying the protein spots and digesting obtained protein with trypsin;\n
i. analyzing eluted dried peptides by mass spectroscopy to obtain identities of proteins;\n
j. comparing the expression of each short-listed protein spot in non malignant and transformed epithelial cells to identity a set of co expressed differentiator marker proteins for the transformed epithelium from the group consisting of lactate dehydrogenase, alpha enolase, prohibitin, cathepsin D, apolipoprotein A-I, tumor protein translationally controlled-1, a stratifin (SFN) family protein, 14-3-3σ, tropomyosin and 14-3-3ζ (YWHAZ); and\n
k. identifying key differentiator protein spots using Linear Discriminator Analysis."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["A method as claimed in claim 1, wherein the key differentiator protein spots identified in step 14(k) respectively comprise the following proteins: 14-3-3σ, lactate dehydrogenase, and apolipoprotein A-I, and wherein upregulation of 14-3-3σ and downregulation of apolipoprotein is indicative of the presence of cancer."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["A method for screening, detection, prognosis, or preparing a treatment regimen for cancer of gingivo-buccal complex in a patient, the method comprising the steps of:\n
(a) collecting a sample of epithelium tissue from the patient;\n
(b) a step for assaying the epithelium tissue in the sample to enable generation of an expression profile of a plurality of differentiator proteins comprising at least 14-3-3 σ, lactate dehydrogenase, and apolipoprotein A-I;\n
(c) generating the expression profile of the plurality of differentiator proteins;\n
(d) comparing the expression profile of the proteins generated in step (c) with a profile of the proteins from non-malignant tissue; and\n
(e) determining whether cancer may be present in the gingivo-buccal complex of the patient based on whether there is a difference in the expression profile of the proteins generated in step (c) and the profile of the proteins from non-malignant tissue."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 3, further comprising a step for conducting a biological assay or immunoassay for determining a level in the patient of a protein or proteins selected from the group consisting of 14-3-3 σ, lactate dehydrogenase, apolipoprotein A-I and a combination thereof."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 4, wherein the immunoassay is performed with an antibody against the protein or proteins."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 4, wherein the biological assay or immunoassay identities an upregulation of 14-3-3 σ and a downregulation of apolipoprotein and the method further comprises preparing a treatment regimen for treating the patient for cancer of the gingiva buccal complex based on the identification."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 3, wherein the plurality of differentiator proteins comprises a further protein or proteins in addition to 14-3-3 σ, lactate dehydrogenase, and apolipoprotein A-I."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 7, wherein the further protein or proteins are selected from the group consisting of alpha enolase, prohibitin, cathepsin D, tumor protein translationally controlled-1, a stratifin (SFN) family protein, tropomyosin and 14-3-3 ζ (YWHAZ)."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 8, wherein the assaying comprises an immunoassay to determine a level of the plurality of differentiator proteins in the epithelium tissue of the sample."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 9, wherein the immunoassay is performed with an antibody against the protein or proteins."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["A method for identifying potential therapeutic targets for cancer of gingivo-buccal complex in a patient, the method comprising the steps of:\n
(a) collecting a sample of epithelium tissue from the patient;\n
(b) assaying the epithelium tissue in the sample to enable generation of an expression profile of a plurality of differentiator proteins comprising at least 14-3-3 σ, lactate dehydrogenase, and apolipoprotein A-I;\n
(c) generating the expression profile of the plurality of differentiator proteins;\n
(d) comparing the expression profile of the differentiator proteins generated in step (c) with a profile of the proteins from non-malignant tissue and determining whether cancer may be present in the gingivo-buccal complex of the patient based on whether there is a difference in the expression profile of the proteins generated in step (c) and the profile of the proteins in the non-malignant tissue; and\n
(e) if the comparing in step (d) indicates a difference in the expression profile of the proteins generated in step (c) and the profile from non-malignant tissue, a step for conducting a biological assay or immunoassay for determining a level in the patient of one or more of the 14-3-3 σ, lactate dehydrogenase, and apolipoprotein A-I."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 11, comprising conducting an immunoassay for determining a level in the patient of a protein or proteins selected from the group consisting of 14-3-3 σ, lactate dehydrogenase, apolipoprotein A-I and a combination thereof."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 12, wherein the immunoassay is performed with an antibody against at least one of the differentiator proteins."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 11, wherein the biological assay or immunoassay in step (e) identifies an upregulation of 14-3-3 σ and a downregulation of apolipoprotein and the method further comprises preparing a treatment regimen for treating the patient for cancer of the gingiva buccal complex based on the identification."],"number":14,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}