{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"135-738-174-867-912","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"135-738-174-867-912","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8761,"type":"PATENT","title":"University of Chicago","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":5987,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8204,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Searched applicants and Owners= \"Chicago Univ \", \"Univ Chicago\", \"\"Chicago University \" NOT \"loyola\"\", \" \"Univ Chic* NOT \"Loyola\" \".
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Total patents: 4656
Searched applicants and Owners= \"Chicago Univ \", \"Univ Chicago\", \"\"Chicago University \" NOT \"loyola\"\", \" \"Univ Chic* NOT \"Loyola\" \".
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Total patents: 4656
(a) transforming said ciliate with a nucleic acid encoding a fusion protein comprising from N-terminus to C-terminus:\n\n(i) a mucocyst-targeting polypeptide of a mucocyst-targeted protein which is cleaved by a protease endogenous to said mucocyst; and\n(ii) a heterologous polypeptide;\n
wherein expression of said fusion protein results in trafficking of said fusion protein to mucocysts within said ciliate and cleavage of said mucocyst-targeting polypeptide to release said heterologous soluble polypeptide within said mucocysts;\n
(b) stimulating regulated secretion from said mucocysts of said ciliate, whereby an extracellular matrix is formed; and\n
(c) separating said heterologous soluble polypeptide from said extracellular matrix and said ciliates."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["A method for production of a heterologous soluble polypeptide by a ciliate, the method comprising:\n
(a) transforming said ciliate with a nucleic acid encoding a first fusion protein comprising from N-terminus to C-terminus:\n\n(i) a heterologous polypeptide; and\n(ii) at least a mucocyst-targeting polypeptide of a mucocyst-targeted protein;\n
wherein expression of said first fusion protein results in trafficking of said first fusion protein to mucocysts within said ciliate; and\n
wherein an endogenous protease within said mucocysts cleaves a cleavage site within said mucocyst-targeting polypeptide and removes any sequences C-terminal to said cleavage site, thereby producing a second fusion protein within said mucocysts;\n
(b) stimulating regulated secretion from said mucocysts of said ciliate, whereby an extracellular matrix is formed; and\n
(c) separating said heterologous polypeptide from said extracellular matrix and said ciliates."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 2 wherein said first fusion protein further comprises a second protease cleavage site between said heterologous polypeptide and said mucocyst-targeting polypeptide."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 3, further comprising the step of reacting said second fusion protein with a second protease which cleaves said second protease cleavage site after step (b)."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 3, further comprising the step of reacting said second fusion protein with a second protease which cleaves said second protease cleavage site after step (c)."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["A method for producing a desired heterologous polypeptide in a culture of ciliates, the method comprising:\n
(a) expressing a fusion protein comprising said heterologous polypeptide and a polypeptide comprising at least one mucocyst-targeting polypeptide of a mucocyst-targeted protein in said ciliates;\n
(b) stimulating regulated secretion from mucocysts of said ciliates, whereby an extracellular matrix is formed by said secretion;\n
(c) separating said extracellular matrix from said ciliates; and\n
(d) isolating said fusion protein from said extracellular matrix."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of any of claim 1-6 wherein said mucocyst-targeted protein is a Grl protein."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of any of claim 1-6 wherein said mucocyst-targeted protein is selected from the group consisting of a Grl-1 protein, a Grl-2 protein a Grl-3 protein, a Grl-4 protein, a Grl-5 protein, a Grl-6 protein, a Grl-7 protein, a Grl-8 protein, a Grl-9 protein, and a Grl-10 protein."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 8 wherein said mucocyst-targeted protein comprises a pro-domain of said Grl protein."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of any of claim 1-6 wherein said mucocyst-targeted protein is an Igr protein or a granule tip protein."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of any of claim 1-6 wherein said mucocyst-targeted protein is a β/γ crystalline domain or a C-terminal crystallin fold containing protein."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method of any of claim 1-6 wherein said fusion protein further comprises an endoplasmic reticulum-targeting polypeptide N-terminal to said mucocyst-targeting polypeptide."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12 wherein said endoplasmic reticulum-targeting polypeptide is a pre-domain of a Grl protein."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12 wherein said endoplasmic reticulum-targeting polypeptide is heterologous to said mucocyst-targeting polypeptide."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["A method for production of a heterologous soluble polypeptide by a ciliate, the method comprising:\n
(a) transforming said ciliate with a nucleic acid encoding a fusion protein comprising from N-terminus to C-terminus:\n\n(i) a soluble polypeptide endogenous to said mucocyst;\n(ii) a protease cleavage site; and\n(iii) a heterologous polypeptide;\n
wherein expression of said fusion protein results in trafficking of said fusion protein to mucocysts within said ciliate;\n
(b) stimulating regulated secretion from said mucocysts of said ciliate, whereby an extracellular matrix is formed by said secretion;\n
(c) separating said fusion protein from said extracellular matrix and said ciliates; and\n
(d) obtaining said heterologous soluble polypeptide from said fusion protein."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 15 wherein said mucocyst-targeted protein is a β/γcrystalline domain containing or a C-terminal crystallin fold protein."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 15 wherein step (d) comprises the step of reacting said heterologous soluble polypeptide with a protease which cleaves said protease cleavage site."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method of any one of claims 15-17 wherein said fusion protein further comprises an endoplasmic reticulum-targeting polypeptide N-terminal to said heterologous polypeptide."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 18 wherein expression of said fusion protein results in trafficking of said fusion protein to mucocysts within said ciliate and cleavage of said endoplasmic reticulum-targeting polypeptide."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 18 wherein said endoplasmic reticulum-targeting polypeptide is a pre-domain of a Grl protein."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 18 wherein said endoplasmic reticulum-targeting polypeptide is heterologous to said soluble polypeptide endogenous to said mucocyst."],"number":21,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}