{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"124-559-457-804-302","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"124-559-457-804-302"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10838,"type":"PATENT","title":"Michigan State University - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":5685,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8287,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: Michigan AND State AND Univ*
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 4400
Search Applicants and Owners separately: Michigan AND State AND Univ*
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 4400
(i) at least one isolated first nucleic acid molecule operatively linked to a first expression control sequence and a first terminator sequence, said first nucleic acid molecule being selected from the group of nucleic acid molecules consisting of:(a) a nucleic acid molecule having a nucleic acid sequence as shown in any one of SEQ ID NO: 27;(b) a nucleic acid molecule encoding a polypeptide having an amino acid sequence as shown in any one of SEQ ID NO: 28;(c) a nucleic acid molecule having a nucleic acid sequence which is at least 70% identical to the nucleic acid sequence of the nucleic acid molecule of (a) or (b), wherein said nucleic acid molecule encodes a polypeptide having or contemplating plastidic pyruvate kinase activity;(d) a nucleic acid molecule encoding an amino acid sequence being at least 70% identical to an amino acid sequence encoded by the nucleic acid molecule of (a) or (b), wherein said nucleic acid molecule encodes a polypeptide having or contemplating plastidic pyruvate kinase activity; and(e) a nucleic acid molecule being a fragment of any one of (a) to (c), wherein said fragment encodes a polypeptide having or contemplating plastidic pyruvate kinase activity, and
(ii) at least one second isolated nucleic acid molecule operatively linked to a second expression control sequence and a second terminator sequence, said second nucleic acid molecule being selected from the group of nucleic acid molecules consisting of:"],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The polynucleotide of claim 1, wherein said polynucleotide comprises further(a) a nucleic acid molecule having a nucleic acid sequence as shown in any one of SEQ ID NO: 23;(b) a nucleic acid molecule encoding a polypeptide having an amino acid sequence as shown in any one of SEQ ID NO: 24;(c) a nucleic acid molecule having a nucleic acid sequence which is at least 70% identical to the nucleic acid sequence of the nucleic acid molecule of (a) or (b), wherein said nucleic acid molecule encodes a polypeptide having or contemplating plastidic pyruvate kinase activity;(d) a nucleic acid molecule encoding an amino acid sequence being at least 70% identical to an amino acid sequence encoded by the nucleic acid molecule of (a) or (b), wherein said nucleic acid molecule encodes a polypeptide having or contemplating plastidic pyruvate kinase activity; and(e) a nucleic acid molecule being a fragment of any one of (a) to (d), wherein said fragment encodes a polypeptide having or contemplating plastidic pyruvate kinase activity.
(iii) at least one third isolated nucleic acid molecule operatively linked to a third expression control sequence and a third terminator sequence, said second nucleic acid molecule being selected from the group of nucleic acid molecules consisting of:"],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The polynucleotide of claim 1 or 2, wherein said first, second and/or third expression control sequence(s) allow for a seed specific expression of the said first, second or third nucleic acid."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The polynucleotide of any one of claims 1 to 3, wherein said first, second and/or third terminator sequence(s) are biologically active in seeds."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The polynucleotide of any one of claims 1 to 4, wherein said first expression control sequence has a nucleic acid sequence as shown in SEQ ID NO: 31."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The polynucleotide of any one of claims 1 to 5, wherein said second expression control sequence has a nucleic acid sequence as shown in SEQ ID NO: 30."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The polynucleotide of any one of claims 2 to 6, wherein said third expression 40 control sequence has a nucleic acid sequence as shown in SEQ ID NO: 29."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The polynucleotide of any one of claims 1 to 7, wherein said first terminator sequence has a nucleic acid sequence as shown in SEQ ID NO: 32 or 34."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The polynucleotide of any one of claims 1 to 8, wherein said second terminator sequence has a nucleic acid sequence as shown in SEQ ID NO: 35."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The polynucleotide of any one of claims 2 to 9, wherein said third terminator sequence has a nucleic acid sequence as shown in SEQ ID NO: 33."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["A vector comprising the polynucleotide of any one of claims 1 to 10."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["A method for the manufacture of a pyruvate kinase polypeptide being capable of increasing the seed storage compound content when expressed in transgenic plants comprising:(a) a nucleic acid molecule having a nucleic acid sequence as shown in any 10 one of SEQ ID NOs: 1,3,5,9,11,13,17,19,25 and 27;(b) a nucleic acid molecule encoding a polypeptide having an amino acid sequence as shown in any one of SEQ ID NOs: 2, 4, 6, 10, 12, 14, 18,20, 26, and 28;(c) a nucleic acid molecule having a nucleic acid sequence which is at least 70% identical to the nucleic acid sequence of the nucleic acid molecule of (a) or (b), wherein said nucleic acid molecule encodes a polypeptide having or contemplating plastidic pyruvate kinase activity;(d) a nucleic acid molecule encoding an amino acid sequence being at least 70% identical to an amino acid sequence encoded by the nucleic acid molecule of (a) or (b), wherein said nucleic acid molecule encodes a polypeptide having or contemplating plastidic pyruvate kinase activity; and(e) a nucleic acid molecule being a fragment of any one of (a) to (d), wherein said fragment encodes a polypeptide having or contemplating plastidic pyruvate kinase activity.
(a) expressing the polynucleotide of any one of claims 1 to 10 in a host cell under conditions allowing assembly of the pyruvate kinase polypeptide; and
(b) obtaining the said assembled pyruvate kinase polypeptide from the host cell."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["An essentially purified assembled pyruvate kinase polypeptide comprising the polypeptides encoded by the polynucleotide of any one of claims 1 to 10 or which is obtainable by the method of claim 12."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["An antibody which specifically recognizes the assembled pyruvate kinase polypeptide of claim 13."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["A host cell comprising the polynucleotide of any one of claims 1 to 10, the vector of claim 11 or the assembled pyruvate kinase polypeptide of claim 13."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["A transgenic non-human organism comprising the polynucleotide of any one of claims 1 to 10, the vector of claim 11, the host cell of claim 12 or the assembled pyruvate kinase polypeptide of claim 13."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The transgenic non-human organism of claim 16, wherein said non-human transgenic organism is a plant or seed."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["A seed comprising the assembled pyruvate kinase polypeptide of claim 13, in its endosperm, embryo plastids or cytosol, wherein said seed is further characterized in that it has:
(a) an increase in oil content in comparison to an isogenic seed,
(b) an increase in embryo weight in comparison to an isogenic seed,
(c) an increase in the amino acid content in comparison to an isogenic seed for an amino acid selected from the group consisting of: aspartic acid, threonine, glycine, cysteine, valine, methionine, isoleueine, histidine, lysine, arginine, and tryptophan, and/or
(d) an increase in protein content."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["A method for the manufacture of a seed oil comprising the steps of:
(a) cultivating the host cell of claim 12, the transgenic non-human organism of claim 16 or a host cell or transgenic non-human organism comprising the assembled pyruvate kinase of claim 14 under conditions allowing synthesis of the said seed oil; and
(b) obtaining the said seed oil from the host cell or the transgenic non-human organism, said seed storage compound being oil, fatty acids and/or lipids."],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["A method for the manufacture of a plant having a modified amount of a seed oil comprising the steps of:
(a) introducing the polynucleotide of any one of claims 1 to 10 or the vector of claim 11 into a plant cell; and
(b) generating a transgenic plant from the said plant cell, wherein the polypeptide encoded by the polynucleotide modifies the amount of the said seed oil in the transgenic plant, said seed storage compound being oil, fatty acids and/or lipids."],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["A method for the manufacture of a plant having a modified amount of a seed oil comprising the steps of:
(a) obtaining a plant cell comprising the assembled pyruvate kinase polypeptide of claim 13; and
(b) generating a transgenic plant from the said plant cell, wherein the polypeptide encoded by the polynucleotide modifies the amount of the said seed oil in the transgenic plant, said seed storage compound being oil, fatty acids and/or lipids."],"number":21,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 20 or 21, wherein the amount of said seed oil is increased compared to a non-transgenic control plant, said seed storage compound being oil, fatty acids and/or lipids."],"number":22,"annotation":false,"title":false,"claim":true},{"lines":["Use of the transgenic non-human organism of claim 16 or 17 or the seed of claim 18 for the manufacture of food or feed."],"number":23,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}