{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"122-207-179-137-818","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"122-207-179-137-818"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8901,"type":"PATENT","title":"University of Michigan Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":14369,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8216,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners= \"Univ Michigan\", \" Michigan Univ\", \" Univ Michigan NOT state NOT tech NOT Eastern\", \"Univ Michigan NOT technological\", \" Univ Michigan NOT Western\"
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Total patent : 14104
Search Applicants and Owners= \"Univ Michigan\", \" Michigan Univ\", \" Univ Michigan NOT state NOT tech NOT Eastern\", \"Univ Michigan NOT technological\", \" Univ Michigan NOT Western\"
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Total patent : 14104
a) providing:\n\ni) a first sample comprising a plurality of proteins;\nii) a second sample comprising a plurality of proteins;\niii) a separating apparatus, wherein said separating apparatus separates proteins based on a physical property;\niv) a mass spectroscopy apparatus; and\n
b) treating said first and second samples with said separating apparatus to produce a first separated protein sample and a second separated protein sample, wherein said first and second separated protein samples are collected from said separating apparatus in a plurality of fractions, each of said fractions defined by a physical property; and\n
c) analyzing said plurality of fractions from each of said first and second separated protein samples with said mass spectroscopy apparatus to produce a protein profile map for each of said first and second samples, wherein said protein profile maps display protein abundance and mass of said first protein sample and said second protein sample, and wherein said protein profile maps displays each protein as a separate band corresponding to said mass of said first protein sample and said second protein sample, and wherein the intensity of said band corresponds to said protein abundance of said first protein sample and said second protein sample; and wherein said protein profile maps for each of said first and second samples are displayed side by side."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, further comprising an automated sample handling device operably linked to said separating apparatus and said mass spectroscopy apparatus, wherein said sample handling device transfers said first and second samples to said separating apparatus, and wherein said sample handling device transfers said first and second separated protein samples from said separating apparatus to said mass spectroscopy apparatus."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2, further comprising a centralized control network operably linked to said automated sample handling device, said separating apparatus, and said mass spectroscopy apparatus, wherein said centralized control network controls the operations of said automated sample handling device, said separating apparatus, and said mass spectroscopy apparatus."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, wherein said centralized control network comprises computer memory and a computer processor."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said first sample comprises a cell lysate from a first cell type and said second sample comprises a cell lysate from second cell type."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 5, wherein said first cell type is a cancerous cell type and said second cell type is a non-cancerous cell type."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said bands are bands of different colors."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said protein abundance and mass are indicative of the cell type of said protein sample."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, further comprising the step of d) determining the identity of individual bands on said protein profile map."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 6, further comprising the step of treating said first sample with an external agent prior to treating said first and second samples with said separating apparatus."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 10, wherein said external agent comprises estradiol."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2, wherein said automated sample handling device comprises a switchable, multi-channel valve."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said first and second samples further comprise a buffer, wherein said plurality of proteins are solubilized in said buffer and wherein said buffer is compatible with said separating apparatus and said mass spectroscopy apparatus."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 13, wherein said buffer comprises a compound of the formula n-octyl C6–C12 glycopyranoside."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 14, wherein said compound of the formula n-octyl C6–C12 glycopyranoside is selected from n-octyl β-D-glucopyranoside and n-octyl β-D-galactopyranoside."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said separating apparatus comprises a liquid phase separating apparatus."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 16, wherein said liquid phase separating apparatus comprises a reverse phase HPLC separating apparatus."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 17, wherein said reverse phase HPLC comprises non-porous reverse phase HPLC."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein prior to said analyzing said first and second separated protein samples by mass spectroscopy, said first and second samples are divided into first and second portions and wherein said second portions are subjected to enzymatic digestion."],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said analyzing said first and second separated protein samples by mass spectrometry comprises analyzing said samples by electrospray ionization-orthogonal acceleration-time-of-flight mass spectrometry."],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said analyzing said first and second separated protein samples by mass spectrometry comprises analyzing said samples by a technique selected from the group consisting of ion trap mass spectrometry, ion trap/time-of-flight mass spectrometry, quadrupole and triple quadrupole mass spectrometry, Fourier Transform (ICR) mass spectrometry, and magnetic sector mass spectrometry."],"number":21,"annotation":false,"title":false,"claim":true},{"lines":["A method of comparing protein profile maps, comprising:\n
a) providing:\n\ni) a cell lysate derived from a cell of unknown type, said cell lysate comprising a plurality of proteins;\nii) a first protein profile map generated by the method of claim 1;\niii) a separating apparatus, wherein said separating apparatus separates proteins based on a physical property; and\niv) a mass spectroscopy apparatus; and\n
b) treating said cell lysate with said separating apparatus to produce a separated protein sample; wherein said separated protein sample is collected from said separating apparatus in a plurality of fractions, each of said fractions defined by a physical property;\n
c) analyzing said plurality of fractions from said separated protein sample with said mass spectroscopy apparatus to produce a second protein profile map, wherein said second protein profile maps displays each protein as a separate band corresponding to said mass of said first protein sample and said second protein sample, and wherein the intensity of said band corresponds to said protein abundance of said first protein sample and said second protein sample; and\n
d) comparing said first protein profile map and said second protein profile map, wherein said first and second protein profile maps are displayed side by side."],"number":22,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 22, wherein said first protein profile map displays protein abundance and mass from cell lysates of several known cell types and said second protein profile map displays protein abundance and mass from said cell lysate of unknown type."],"number":23,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 22, wherein said bands are bands of different colors."],"number":24,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 23, wherein said protein abundance and mass are indicative of a cell identity."],"number":25,"annotation":false,"title":false,"claim":true},{"lines":["A system for the production of a data representation of a protein profile map, comprising:\n
a) a non-porous reverse phase HPLC separating apparatus;\n
b) an automated sample handling apparatus configured to receive first and second separated protein samples from said reverse phase HPLC separating apparatus;\n
c) a mass spectroscopy apparatus configured to receive proteins from said automated sample handling apparatus;\n
d) a processor configured to produce a data representation of a protein profile map for said first and second separated protein samples analyzed by said mass spectroscopy apparatus, wherein said protein profile map displays protein abundance and mass of a separated protein sample, wherein said protein profile map displays proteins as separate bands corresponding to said protein abundance and mass of said separated protein sample, and wherein the intensity of said bands corresponds to the abundance of said proteins, wherein said protein profile maps for each of said first and second samples are displayed side by side; and\n
e) a display apparatus that displays said protein profile maps."],"number":26,"annotation":false,"title":false,"claim":true},{"lines":["The system of claim 26, wherein said protein profile map displays protein abundance as bands of varying intensity."],"number":27,"annotation":false,"title":false,"claim":true},{"lines":["The system of claim 27, wherein said protein abundance is expressed as bands of different colors."],"number":28,"annotation":false,"title":false,"claim":true},{"lines":["The system of claim 26, wherein said protein abundance and mass are indicative of a cell type of said protein sample."],"number":29,"annotation":false,"title":false,"claim":true},{"lines":["The system of claim 26, wherein said processor is configured to determine the identity of individual bands on said protein profile map."],"number":30,"annotation":false,"title":false,"claim":true},{"lines":["The system of claim 26, wherein said automated sample handling device comprises a switchable, multi-channel valve."],"number":31,"annotation":false,"title":false,"claim":true},{"lines":["The system of claim 26, wherein said mass spectrometry apparatus comprises a electrospray ionization-orthogonal acceleration-time-of-flight mass spectrometry apparatus."],"number":32,"annotation":false,"title":false,"claim":true},{"lines":["A method of producing protein profile maps, comprising:\n
a) providing:\n\ni) a first sample comprising a plurality of proteins;\nii) a second sample comprising a plurality of proteins;\niii) a separating apparatus, wherein said separating apparatus separates proteins based on a physical property;\niv) a mass spectroscopy apparatus; and\n
b) treating said first and second samples with said separating apparatus to produce a first separated protein sample and a second separated protein sample, wherein said first and second separated protein samples are collected from said separating apparatus in a plurality of fractions, each of said fractions defined by a physical property;\n
c) analyzing said plurality of fractions from each of said first and second separated protein samples with said mass spectroscopy apparatus to produce first and second protein profile maps for each of said first and second protein samples, wherein said protein profile maps display protein abundance and mass of said first protein sample and said second protein sample; and\n
d) displaying a differential display protein map of said first and second protein profile maps, wherein said differential display protein map displays the difference in protein abundance versus mass between proteins in said first and second protein samples, and wherein said differential display protein profile map displays the difference in protein abundance between each protein as a separate band corresponding to said mass of said first protein sample and said second protein sample, and wherein the intensity of said band corresponds to the difference in protein abundance."],"number":33,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 33, further comprising the step of displaying said first and second protein profile maps."],"number":34,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 34, wherein said first and second protein profile maps and said differential display map are displayed side by side."],"number":35,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}