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Search Applicants and Owners separately: colorado state uni*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1775
Search Applicants and Owners separately: colorado state uni*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1775
(i) providing a cultured diploid ungulate differentiated cell;\n
(ii) inserting an exogenous DNA into said cell;\n
(iii) selecting for integration of said exogenous DNA in said cell;\n
(iv) inserting the nucleus of said cell into an enucleated, metaphase II-arrested oocyte of the same species to reconstruct an embryo capable of developing to term;\n
(v) activating the reconstructed embryo;\n
(vi) transferring the activated embryo into a recipient female of the same species; and\n
(vii) generating a transgenic ungulate from the reconstructed embryo."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said exogenous DNA is introduced into said cell by transformation, transfection, or microinjection."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said ungulate is bovine."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said ungulate is a sheep."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said ungulate is a pig."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said ungulate is a goat."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said cell is a fibroblast cell."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["A method of producing a transgenic ungulate comprising:\n
(i) providing a cultured diploid ungulate differentiated cell;\n
(ii) deleting, duplicating, or modifying the endogenous DNA of said cell to genetically modify said cell;\n
(iii) selecting for a genetically modified cell;\n
(iv) inserting the nucleus of said genetically modified cell into an enucleated, metaphase II-arrested oocyte of the same species to reconstruct an embryo capable of developing to term;\n
(v) activating the reconstructed embryo;\n
(vi) transferring the activated embryo into a recipient female of the same species; and\n
(vii) generating a transgenic ungulate from the reconstructed embryo."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 8, wherein said cell is a fibroblast cell."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 8, wherein said ungulate is bovine."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 8, wherein said ungulate is a sheep."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 8, wherein said ungulate is a pig."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 8, wherein said ungulate is a goat."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["A method of producing a transgenic ungulate comprising:\n
(i) providing an activated, transgenic, reconstructed embryo comprising a nucleus from a cultured diploid ungulate differentiated cell;\n
(ii) culturing said reconstructed embryo; and\n
(iii) transferring the cultured embryo into a recipient female of the same species; and\n
(iv) developing the transferred embryo to term."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14, wherein said ungulate is bovine."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14, wherein said ungulate is a sheep."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14, wherein said ungulate is a pig."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14, wherein said ungulate is a goat."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["A method of producing a transgenic ungulate embryo comprising:\n
(i) providing an activated, transgenic, reconstructed embryo comprising a nucleus from a cultured diploid ungulate differentiated cell; and\n
(ii) culturing said reconstructed embryo; wherein said embryo is capable of developing to term."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 19, wherein said ungulate is bovine."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 19, wherein said ungulate is a sheep."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 19, wherein said ungulate is a pig."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 19, wherein said ungulate is a goat."],"number":23,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}