{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"112-992-062-464-78X","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"112-992-062-464-78X"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11813,"type":"PATENT","title":"University of Aberdeen Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":27926,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8443,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: univ* aberdeen. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1658
Search Applicants and Owners separately: univ* aberdeen. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1658
culturing cocoa callus cells derived from a Theobroma cacao plant in a culture medium for a time sufficient and under conditions sufficient to result in production of cocoa oligomeric procyanidins at a first rate; and\n
inducing the cells to produce the cocoa oligomeric procyanidins at a second rate that is higher than the first rate by introducing a selected amount of a carbohydrate to the cells, the cells producing greater than 100 mg of procyanidins per liter of cell culture, greater than 200 mg of procyanidins per liter of packed cells, and less than 0.5 ug caffeine and less than 0.75 ug theobromine per mg of procyanidins, thereby preparing cocoa oligomeric procyanidins,\n
wherein said step of culturing the cocoa cells is carried out in the presence of dissolved oxygen concentration at 1% to 400% of air saturation."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, further comprising extracting the cocoa oligomeric procyanidins from the cells after the inducing step."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4, wherein the extracting occurs between 1 day to 10 days after introduction of the carbohydrate."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4, wherein the extracting is carried out with an ethanol-based extraction solution or an acidic extraction solution, or both."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, wherein the carbohydrate comprises glucose."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, wherein the carbohydrate is introduced during or after a last phase of an exponential growth state."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, wherein the culture medium is a hormone-free medium."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 9, further comprising introducing a carbohydrate to the cells in the hormone-free medium in an amount sufficient for inducing the cells to produce the cocoa oligomeric procyanidins at a third rate that is higher than the second rate."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, wherein introduction of the carbohydrate is in an amount of about 0.5% to about 20% by volume of the culture medium and results in one or more of stable cell line maintenance, induction of overproduction of the cocoa oligomeric procyanidins, or prevention of cell aggregation."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, wherein said step of inducing the cells is carried out in the presence of dissolved oxygen concentration at 1% to 400% of air saturation."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, further comprising one or more of the following:\n
harvesting the cells;\n
homogenizing cell biomass in a suitable solvent for extraction of polyphenol rich fraction;\n
isolating a procyanidin rich fraction using solvent-solvent extraction and/or chromatography; or\n
drying or concentrating the procyanidin fraction."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, further comprising one or more of the following:\n
growing callus cells derived from a plant belonging to the genus Theobroma on solid growth medium;\n
selecting a rapidly growing cell line from a callus culture comprising cells derived from a plant belonging to the genus Theobroma; or\n
initiating a cell suspension culture by inoculating the rapidly growing cell line into liquid medium."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["A method of preparing cocoa oligomeric procyanidins, the method comprising:\n
culturing cocoa callus cells derived from a Theobroma cacao plant in a hormone-free medium for a time sufficient and under conditions sufficient for inducing the cells to produce cocoa oligomeric procyanidins at a first rate; and\n
inducing the cells to produce the cocoa oligomeric procyanidins at a second rate that is higher than the first rate by introducing a selected amount of a carbohydrate to the cells, the cells producing greater than 100 mg of procyanidins per liter of cell culture, greater than 200 mg of procyanidins per liter of packed cells, and less than 0.5 ug caffeine and less than 0.75 ug theobromine per mg of procyanidins, thereby preparing cocoa oligomeric procyanidins."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 15, further comprising extracting the cocoa oligomeric procyanidins from the cells after the inducing step."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 15, wherein the carbohydrate comprises glucose."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 15, wherein introduction of the carbohydrate is in an amount of about 0.5% to about 20% by volume of the culture medium and results in one or more of stable cell line maintenance, induction of overproduction of the cocoa oligomeric procyanidins, or prevention of cell aggregation."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 15, wherein said step of culturing the cocoa cells is carried out in the presence of dissolved oxygen concentration at 1% to 400% of air saturation during a growth stage."],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 15, wherein said step of inducing the cocoa cells is carried out in the presence of dissolved oxygen concentration at 1% to 400% of air saturation."],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["The isolated cocoa cell line of claim 1, wherein the callus cells are selected to have a doubling time between 10 and 15 days and are selected to grow to a biomass concentration between 40% and 69% packed cell volume in cell suspension culture."],"number":21,"annotation":false,"title":false,"claim":true},{"lines":["The isolated cocoa cell line of claim 1, wherein the procyanidins include oligomeric procyanidins selected from the group consisting of dimers through dodecamers."],"number":22,"annotation":false,"title":false,"claim":true},{"lines":["The isolated cocoa cell line of claim 1, wherein the callus cells are selected to produce at least 250 mg of procyanidins per liter of cell culture and greater than 500 mg of procyanidins per liter of packed cells."],"number":23,"annotation":false,"title":false,"claim":true},{"lines":["The isolated cocoa cell line of claim 1, wherein the callus cells are selected to produce at least 1600 mg of procyanidins per liter of cell culture and greater than 5000 mg of procyanidins per liter of packed cells."],"number":24,"annotation":false,"title":false,"claim":true},{"lines":["A cell suspension culture, comprising:\n
the cocoa callus cells of claim 1; and\n
a suspension medium,\n
the callus cells growing in the suspension medium, the suspension medium comprising a carbon source, a nitrogen source, and one or more supplements selected from the group consisting of a macronutrient, a micronutrient, an auxin, a cytokinin, a vitamin, an amino acid, a hormone, inositol, and a myo-inositol, the callus cells producing greater than 100 mg of procyanidins per liter of cell culture, greater than 200 mg of procyanidins per liter of packed cells, and less than 0.5 ug caffeine and less than 0.75 ug theobromine per mg of procyanidins in the suspension medium."],"number":25,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}