{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"108-908-814-265-937","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"108-908-814-265-937"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11655,"type":"PATENT","title":"University of Freiburg - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":2803,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8321,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
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a) cloning random genomic DNA fragments from at least one organism of interest upstream of a promoter-less reporter gene into a vector, in order to generate a random genomic library,
b) transforming said random genomic library into a host strain,
c) selecting clones with anti-infective-responsive reporter expression from said host strains, comprising treating said clones with eitheri) at least one known anti-infective substance, orii) at least one potentially anti-infective substance, andiii) comparing said treated clones from i) or ii) with at least one untreated control sample, and
d) identifying those clones exhibiting a different anti-infective-responsive reporter expression as biosensors."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method for producing a biosensor for an in vitro screening system for identifying anti-infective substances according to claim 1, wherein said at least one organism of interest is selected from fungi, protozoans, parasites, viruses and bacteria, such as pathogenic bacteria, in particular human or animal pathogenic bacteria, Gram-positive and Gram-negative bacteria."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method for producing a biosensor for an in vitro screening system for identifying anti-infective substances according to claim 1 or 2, wherein said at least one known anti-infective substance is selected from ampicillin, kanamycin, evemimicin, fluoroquinolones, bacitracin, cefotaxim, cefsulodin, cycloserine, fosfomycin, imipenem, vancomycin, cerulenin, thiolactomycin, triclosan (Irgasan), avilamycin, azithromycin, blasticidin, chloramphenicol, puromycin, gentamycin, hygromycin, neomycin, streptomycin, tetracycline, actinomycin, ciprofloxacin, novobiocin, phleomycin, rifampicin, and polymyxin."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method for producing a biosensor for an in vitro screening system for identifying anti-infective substances according to any of claims 1 to 3, further comprising analyzing
a) the specificity and sensitivity of said reporter gene expression in response to at least one known anti-infective substance having a defined mode of anti-infective action, and/or
b) the reporter gene expression in response to at least one cellular stress condition."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method for producing a biosensor for an in vitro screening system for identifying anti-infective substances according to claim 4, wherein said defined mode of anti-infective action and/or cellular stress condition is selected from inhibiting bacterial functions that are essential in vitro and in vivo during infection, such as cell wall and protein biosynthesis, peptidoglycan biosynthesis, protein biosynthesis, fatty acid biosynthesis, DNA replication, RNA polymerase, division functions; DNA replication/transcription/intercalation, disruption of the cell membrane, cellular lysis, chelating agents, oxidizers, solvents, and protein denaturation."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method for producing a biosensor for an in vitro screening system for identifying anti-infective substances according to any of claims 1 to 5, further comprising combining at least two complimentary biosensors as identified."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["An in vitro screening system for identifying anti-infective substances, comprising a biosensor produced according to any of claims 1 to 6."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The in vitro screening system for identifying anti-infective substances according to claim 7, comprising at least two complimentary biosensor clones produced according to any of claims 1 to 6, and optionally comprising at least one biosensor clone comprising an anti-infective-responsive reporter expression identified through transcriptome/proteome-based selection, with the provisio that at least one biosensor clone has not been identified through said transcriptome/proteome-based selection."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The in vitro screening system for identifying anti-infective substances according to claim 7 or 8, comprising a mixture of biosensors."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The in vitro screening system for identifying anti-infective substances according to any of claims 7 to 9, wherein the combination of biosensors is specifically selected for identifying cell wall biosynthesis inhibitors, fatty acid biosynthesis inhibitors, RNA polymerase inhibitors, gyrase inhibitors, topoisomerase inhibitors, protein biosynthesis inhibitors, protein biosynthesis inhibitors that bind to the ribosome at a site close to the avilamycin binding-site, or antibacterial compounds active against Gram-negative and/or Gram-positive bacterial pathogens, Mycobacteria, Treponema, and/or Mollicutes."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["An in vitro method for screening anti-infective substances comprising contacting at least one potentially anti-infective substance with the in vitro screening system according to any of claims 7 to 10, and identifying the potentially anti-infective substance based on a difference in the anti-infective-responsive reporter expression, compared to a control."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The in vitro method for screening anti-infective substances according to claim 11, wherein a mixture of individual biosensors is combined and their responses measured in parallel."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The in vitro method for screening anti-infective substances according to claim 12, wherein multiple fluorescent proteins are detected in parallel using flow cytometry, fluorescence plate readers, and/or bioluminescence plate readers, and /or wherein additional parameters, such as biosensor cell number, biosensor cell size, biosensor membrane properties, are recorded."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["A method for generating a response profile for the anti-infective substance screened according to claim 12 or 13 comprising combining the response data from several different biosensors, and optionally further comprising comparing and/or combining said response profile as generated with a response profile for a set of antibacterial molecules with known modes of action."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["Use of the in vitro screening system according to any of claims 7 to 10 for screening anti-infective substances."],"number":15,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}