{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"104-349-130-045-438","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"104-349-130-045-438","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8906,"type":"PATENT","title":"Univ Pennsylvania Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":24238,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8221,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search applicants and owners= \"Univ Pennsylvania\", \"Pennsylvania Univ\", \"Univ Penns*\", \"Penn* Univ\", \"University of Pennsylvania\", \"Pennsylvania University\".
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Search applicants and owners= \"Univ Pennsylvania\", \"Pennsylvania Univ\", \"Univ Penns*\", \"Penn* Univ\", \"University of Pennsylvania\", \"Pennsylvania University\".
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(a) providing an injectable vaccine which comprises in a sterile aqueous solution in admixture:
(i) intracellular cytoplasmic antigens separated from disrupted cells Pythium insidiosum by SDS-PAGE; and
(ii) extracellular antigens secreted into a medium for growing the cells of Pythium insidiosum wherein the mixture comprises 28, 30 and 32 kDa antigens as determined by SDS-PAGE; and
(b) vaccinating the mammal with the vaccine."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the antigens have been provided by
(a) growing cells of the Pythium insidiosum in a culture medium and then
(i) killing the cells;
(ii) separating the killed cells from the culture medium so as to produce a first supernatant comprising the extracellular antigens secreted into the medium; and
(iii) disrupting the cells water to provide the intracellular cytoplasmic antigens in a second supernatant which is separated from the disrupted cells; and
(b) separating the extracellular antigens from the first supernatant."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the cells have been disrupted by sonication."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the Pythium insidiosum is deposited as ATCC 74446."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of any one of claims 2 , 3 , or 4 wherein the culture medium is Sabouraud dextrose broth."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 2 wherein the cells are killed with thimersol."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 2 wherein disrupted cells are separated from the culture medium for the cells by centrifugation."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 2 wherein the intracellular cytoplasmic antigens in the second supernatant and the extracellular antigens in the first supernatant are mixed to provide a mixture of antigens, precipitating the mixture of antigens with acetone to provide a precipitate, dissolving the precipitate in sterile distilled water to provide a solution of the antigens, and dialyzing the solution of antigens in sterile distilled water to remove low molecular weight components less than 10,000 MW to provide the vaccine."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the mammal after vaccination is monitored for a change in a Th1 response and a Th2 response, wherein an increase in Th1 response and a decrease in the Th2 indicates that the mammal has developed the Th1 response to the vaccine."],"number":9,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}