{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"099-586-407-015-953","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"099-586-407-015-953"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10731,"type":"PATENT","title":"The University of North Carolina at Chapel Hill - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":8887,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8232,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately:\"uni* north caro*\". Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1000
Search Applicants and Owners separately:\"uni* north caro*\". Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1000
contacting Ring2 with a protein substrate in the presence of a test compound; and\n
detecting the level of ubiquitination of the protein substrate under conditions sufficient to provide ubiquitination of the protein substrate, wherein a change in ubiquitination of the protein substrate as compared with the level of ubiquitination of the protein substrate in the absence of the test compound indicates that the test compound is a modulator of the ubiquitin E3 ligase activity of Ring2."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the method is a method of identifying a compound that modulates histone ubiquitin E3 ligase activity of Ring2."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2, wherein the method is a method of identifying a compound that modulates histone H2A ubiquitin E3 ligase activity of Ring2."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, wherein the method is a method of identifying a compound that modulates histone H2A-K119 ubiquitin E3 ligase activity of Ring2."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2, wherein the histone substrate is a core histone, a mononucleosome, a dinucleosome or an oligonucleosome."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the Ring2 is a recombinant protein."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein a reduction in ubiquitination of the protein substrate as compared with the level of ubiquitination of the protein substrate in the absence of the test compound indicates that the test compound is an inhibitor of the ubiquitin E3 ligase activity of Ring2."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein an increase in ubiquitination of the protein substrate as compared with the level of ubiquitination of the protein substrate in the absence of the test compound indicates that the test compound is an activator of the ubiquitin E3 ligase activity of Ring2."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the method is a cell-free method."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["A method of identifying a compound that modulates ubiquitin E3 ligase activity of a complex comprising Ring2, the method comprising:\n
contacting the complex with a protein substrate in the presence of a test compound; and\n
detecting the level of ubiquitination of the protein substrate under conditions sufficient to provide ubiquitination of the protein substrate, wherein a change in ubiquitination of the protein substrate as compared with the level of ubiquitination of the protein substrate in the absence of the test compound indicates that the test compound is a modulator of the ubiquitin E3 ligase activity of the complex."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 10, wherein the complex comprises Ring2, Ring1, Bmi1 and HPH2."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 10, wherein the complex comprises dRing1, Psc and ph."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 10, wherein the method is a method of identifying a compound that modulates histone ubiquitin E3 ligase activity of the complex."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 13, wherein the method is a method of identifying a compound that modulates histone H2A ubiquitin E3 ligase activity of the complex."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 14, wherein the method comprises detecting the level of H2A-K119 ubiquitination."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 10, wherein the complex is a recombinant complex."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 10, wherein a reduction in ubiquitination of the protein substrate as compared with the level of ubiquitination of the protein substrate in the absence of the test compound indicates that the test compound is an inhibitor of the ubiquitin E3 ligase activity of the complex."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 10, wherein an increase in ubiquitination of the protein substrate as compared with the level of ubiquitination of the protein substrate in the absence of the test compound indicates that the test compound is an activator of the ubiquitin E3 ligase activity of the complex."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["A method of identifying a candidate compound for treating cancer, the method comprising:\n
contacting Ring2 with a histone H2A substrate in the presence of a test compound; and\n
detecting the level of ubiquitination of the histone H2A substrate under conditions sufficient to provide histone H2A ubiquitination, wherein a reduction in histone H2A ubiquitination as compared with the level of histone H2A ubiquitination in the absence of the test compound indicates that the test compound is a candidate compound for the treatment of cancer."],"number":19,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}