{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"087-884-570-651-52X","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"087-884-570-651-52X","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8909,"type":"PATENT","title":"Cornell Univ Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":11969,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8223,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search applicants and owners= \"Cornell Univ\", \" Univ Cornell\", \" Cornell Res* Found*\", \"Corne* Univ* Tech* Enter* Comm*\".
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Total patent: 10677
Search applicants and owners= \"Cornell Univ\", \" Univ Cornell\", \" Cornell Res* Found*\", \"Corne* Univ* Tech* Enter* Comm*\".
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Total patent: 10677
i) a recombinant vector encoding a fusion protein comprising a purification tag and\n
ii) a small ubiquitin-like molecule (SUMO) cleavable by an active fragment of Ulp1 protease comprising amino acid residues 403-621 of yeast Ulp1, wherein said vector further comprises a multiple cloning site suitable for cloning a nucleic acid sequence encoding a poly-amino acid of interest, wherein the poly-amino acid of interest is expressed at 1% or less than 1% of total protein by weight or is expressed defectively when it does not contain the small ubiquitin-like molecule (SUMO) that functions as an expression enhancing amino acid sequence in a recombinant expression system."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The kit according to claim 3, further comprising an antibody that specifically binds an amino acid sequence of the SUMO protein."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The kit according to claim 4, wherein the SUMO protein is Smt3 identified in SEQ ID NO: 3."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["A DNA molecule comprising a nucleotide sequence encoding a small ubiquitin-like molecule (SUMO) cleavable by an active fragment of Ulp1 protease comprising amino acid residues 403-621 of yeast Ulp1, and a poly-amino acid of interest, wherein the poly-amino acid of interest is expressed at 1% or less than 1% of total protein by weight or is expressed defectively when it does not contain the small ubiquitin-like molecule (SUMO) that functions as an expression enhancing amino acid sequence in a recombinant expression system."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The DNA molecule according to claim 6, comprising the nucleotide sequence present from the BamHI restriction site to the NheI restriction site of plasmid pSUMO (ATCC PTA-6647)."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["A DNA vector comprising a nucleotide sequence encoding a small ubiquitin-like molecule (SUMO) cleavable by an active fragment of Ulp1 protease comprising amino acid residues 403-621 of yeast Ulp1, and a poly-amino acid of interest, wherein the poly-amino acid of interest is expressed at 1% or less than 1% of total protein by weight or is expressed defectively when it does not contain the small ubiquitin-like molecule (SUMO) that functions as an expression enhancing amino acid sequence in a recombinant expression system."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["A DNA vector having the nucleotide sequence of plasmid, pSUMO (ATCC PTA-6647)."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["A recombinant vector according to claim 1, wherein the poly-amino acid of interest is an immunoglobulin, a membrane associated protein, a protein or peptide that precipitates in recombinant host cells, or a protein produced in a non-native or inactive form."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["A kit according to claim 3, wherein the poly-amino acid of interest is an immunoglobulin, a membrane associated protein, a protein or peptide that precipitates in recombinant host cells, or a protein produced in a non-native or inactive form."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["A kit according to claim 3, further comprising a nucleic acid sequence encoding a poly-amino acid of interest."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["A recombinant vector according to claim 1, wherein the SUMO comprises amino acid residues 1-98 of SEQ ID NO: 3."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["A recombinant vector according to claim 1, wherein the SUMO comprises amino acid residues 13-101 of SEQ ID NO: 3."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["A recombinant vector according to claim 1, wherein the SUMO comprises amino acid residues 14-98 of SEQ ID NO: 3."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["A kit according to claim 3, wherein the SUMO comprises amino acid residues 1-98 of SEQ ID NO: 3."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["A kit according to claim 3, wherein the SUMO comprises amino acid residues 13-101 of SEQ ID NO: 3."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["A kit according to claim 3, wherein the SUMO comprises amino acid residues 14-98 of SEQ ID NO: 3."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["A DNA molecule according to claim 6, wherein the SUMO comprises amino acid residues 1-98 of SEQ ID NO: 3."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["A DNA molecule according to claim 6, wherein the SUMO comprises amino acid residues 13-101 of SEQ ID NO: 3."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["A DNA molecule according to claim 6, wherein the SUMO comprises amino acid residues 14-98 of SEQ ID NO: 3."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["A DNA vector according to claim 8, wherein the SUMO comprises amino acid residues 1-98 of SEQ ID NO: 3."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["A DNA vector according to claim 8, wherein the SUMO comprises amino acid residues 13-101 of SEQ ID NO: 3."],"number":23,"annotation":false,"claim":true,"title":false},{"lines":["A DNA vector according to claim 8, wherein the SUMO comprises amino acid residues 14-98 of SEQ ID NO: 3."],"number":24,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}