{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"086-193-103-030-311","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"086-193-103-030-311","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10707,"type":"PATENT","title":"Duke Univ Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":9941,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8224,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately :' Duke Univ\"; \" Univ Duke\"; \" Uni* Duk*\"; \" Duk* Uni*\"; ' Duke University\" ; \" University Duke\".
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 8489
Search Applicants and Owners separately :' Duke Univ\"; \" Univ Duke\"; \" Uni* Duk*\"; \" Duk* Uni*\"; ' Duke University\" ; \" University Duke\".
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 8489
(a) the GRP94 ligand binding domain polypeptide consists of amino acids 48-316 of SEQ ID NO: 6;\n
(b) the ligand comprises N-ethylcarboxamidoadenosine (NECA); and\n
(c) the crystallized complex has a crystalline form selected from the group consisting of:\n"],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["A method of generating a crystallized GRP94 ligand binding domain polypeptide complexed to N-ethylcarboxamidoadenosine (NECA), the method comprising:\n(i) a crystalline form with unit cell lattice constants of a=99.889 Å,\nb=89.614 Å, c=60.066 Å; β=90.132°; space group symmetry C2; and 2 GRP94+NECA complexes in the asymmetric unit; and\n(ii) a crystalline form with lattice constants of a=89.200 Å,\nb=99.180 Å, c=63.071 Å; α=β=γ=90.0°; space group symmetry C222(1); and 1 GRP94+NECA complex in the asymmetric unit.\n
(a) preparing a solution containing 30 mg/ml of a GRP94 ligand binding domain polypeptide consisting of amino acids 48-316 of SEQ ID NO: 6 in 10 mM Tris-HCl, pH 7.6, 1 mM DTT, 100 mM NaCl and three molar equivalents of NECA to the GRP94 ligand binding domain polypeptide; and\n
(b) growing a crystal at 18° C. using the hanging drop method by mixing equal volumes of the solution of (a) with a reservoir solution consisting of 100 mM Tris-HCl, pH 7.6, 150-250 mM MgCl2, and 33-38% polyethylene glycol 400 (PEG 400), whereby a crystallized GRP94 ligand binding domain polypeptide complexed to NECA is generated."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["A crystalline composition produced by the method of claim 2."],"number":3,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}