{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"082-970-034-600-728","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"082-970-034-600-728"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":14226,"type":"PATENT","title":"Wellcome Trust Sanger Institute Patent Portfolio","description":"
Search Applicants and Owners separately:\"Genome Research Limited\" ; Genome Res. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 164
Search Applicants and Owners separately:\"Genome Research Limited\" ; Genome Res. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 164
(a) a plurality of human IgH V regions, one or more human D regions and one or more human J regions upstream of the mouse constant region; wherein at least one mouse enhancer is maintained in functional arrangement with the mouse constant region and wherein the human DNA is targeted to the region between the J4 exon and the Cmu locus in the mouse genome IgH locus and
(b) human lambda region variable DNA in functional arrangement with a mouse kappa constant region, for example inserted upstream of a kappa constant region; wherein at least one mouse enhancer is maintained in functional arrangement with the mouse constant region; and wherein the mouse is able to produce a repertoire of chimaeric antibodies, or chimaeric heavy or light chains having a mouse constant region and a human variable region."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["A mouse or mouse cell according to claim 1 wherein the mouse enhancer sequence of
(a) is maintained upstream of the mouse Mu constant region in its native position with respect to the distance from the constant region."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["A mouse or mouse cell according to claim 1 wherein the mouse enhancer sequence of
(a) is maintained downstream of the mouse Mu constant region, suitably in its nativeposition with respect to the distance from the constant region."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["A mouse or mouse cell according to claim 1-3 wherein an endogenous switch sequence is maintained upstream of the mouse Mu constant region in its native position with respect to distance from the constant region of (a)."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["A mouse or mouse cell according to any preceding claim wherein human variable regions are inserted upstream of a sufficient portion of the mouse constant region to allow the formation of an effective chimaeric antibody capable of specifically recognising an antigen."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["A mouse or mouse cell according to any preceding claim modified to prevent expression of fully mouse specific antibodies."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["A mouse or mouse cell according to any preceding claim which is homozygous at one or two immunoglobulin loci for DNA encoding a chimaeric antibody chain."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["A cell according to any preceding claim which is an ES cell, hematopoietic stem cell or iPS cell capable of developing into a mouse, or capable of contributing to tissues and organs of a mouse, the mouse being able to produce a repertoire of antibodies or antibody chains which are chimaeric, said chimaeric antibodies or chains having a mouse constant region and a human variable region."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["An immortalised cell or cell line derived from a cell of any preceding claim."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["A cell or mouse according to any preceding claim comprising a rat switch sequence."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["A method for producing an antibody or antibody heavy or light chain specific to a desired antigen, the method comprising immunizing a mouse according to any preceding claim with the desired antigen and recovering the antibody or antibody chain or recovering a cell producing the antibody or heavy or light chain."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["A mouse according to any one of claims 1 to 7 and 10 which has been immunised with an antigen to produce an antibody or antibody chain."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["A mouse or cell according to any preceding claim wherein the mouse or cell is from the mouse C57BL/6N,C57BL/6J, 129S5 or 129Sv strain."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["A cell according to claim 1-10 which is AB2.1 or JM8."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["A method for producing a fully humanised antibody or antibody chain comprising immunizing a mouse according to claim 1-7 and 10 and then replacing the mouse constant region of an antibody or chimaeric antibody chain specifically reactive with the antigen with a human constant region, optionally by engineering of the nucleic acid encoding the antibody or chimaeric or antibody chain."],"number":15,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}