{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"081-503-348-156-069","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"081-503-348-156-069","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11797,"type":"PATENT","title":"Queen's University at Kingston Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":5666,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8427,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: queen* AND univ*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1261
Search Applicants and Owners separately: queen* AND univ*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1261
(i) a strong ubiquitous promoter;\n
(ii) downstream of said strong ubiquitous promoter, a conditional-STOP cassette comprising a coding region of a protein and polyadenylation signal, flanked on either side by a recombinase recognition site; and\n
(iii) downstream of said conditional-STOP cassette, a sequence encoding a fusion protein comprising a caspase linked in frame with a FK Binding Protein (FKBP) or variant thereof, wherein said fusion protein is not expressed when said conditional-STOP cassette is present in the construct, wherein\n
expression of a recombinase in an auditory hair cell of the mouse results in expression of the fusion protein in the auditory hair cell, and exposure of the mouse to an FKBP dimerizing agent results in hearing loss in the mouse; or\n
expression of a recombinase in pancreatic beta cell of the mouse results in expression of the fusion protein, and exposure of the mouse to an FKBP dimerizing agent results in an increase in a blood glucose level in the mouse."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The transgenic mouse of claim 1, wherein said recombinant nucleic acid molecule is operably linked to one or more additional regulatory sequences."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["A cell isolated from the transgenic mouse of claim 1 having a recombinant nucleic acid molecule stably integrated into the genome of said cell, said recombinant nucleic acid molecule comprising:\n
(i) a strong ubiquitous promoter;\n
(ii) downstream of said strong ubiquitous promoter, a conditional-STOP cassette comprising a coding region of a protein and polyadenylation signal, flanked on either side by a recombinase recognition site; and\n
(iii) downstream of said conditional-STOP cassette, a sequence encoding a fusion protein comprising a caspase linked in frame with a FK Binding Protein (FKBP) or variant thereof, wherein said fusion protein is not expressed when said conditional-STOP cassette is present in the construct, wherein\n
expression of a recombinase in said cell results in expression of the fusion protein in the cell."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The cell of claim 3, wherein said cell is a stem cell or a germ cell."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The transgenic mouse of claim 1, wherein the caspase is caspase 3."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["A method for the production of a transgenic mouse of claim 1, comprising introduction of a recombinant nucleic acid molecule into a germ cell, an embryonic cell, or an egg cell, a recombinant nucleic acid molecule comprising:\n
(i) a strong ubiquitous promoter;\n
(ii) downstream of said strong ubiquitous promoter, a conditional-STOP cassette including a coding region of a protein and polyadenylation signal, flanked on either side by a recombinase recognition site; and\n
(iii) downstream of said conditional-STOP cassette, a sequence encoding a fusion protein comprising a caspase linked in frame with a FK Binding Protein (FKBP) or variant thereof, wherein said fusion protein is not expressed when said conditional-STOP cassette is present in the construct."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6, further comprising crossing said transgenic mouse with a second mouse, wherein an auditory hair cell or a pancreatic beta cell of said second mouse express a recombinase that binds to and excises said stop sequence."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The transgenic mouse of claim 1, wherein said strong ubiquitous promoter is a CMV, β-actin, or ROSA26 promoter."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The transgenic mouse of claim 1, wherein expression of a recombinase in an auditory hair cell of the mouse results in expression of the fusion protein in the auditory hair cell, and exposure of the mouse to an FKBP dimerizing agent results in hearing loss in the mouse."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The transgenic mouse of claim 9, wherein the mouse expresses a recombinase in an auditory hair cell."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The transgenic mouse of claim 10, wherein the recombinase is expressed from a cell-specific promoter."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The transgenic mouse of claim 10, wherein the recombinase is cyclization recombination (Cre) recombinase or flippase (Flp) recombinase."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The transgenic mouse of claim 11, wherein the cell-specific promoter is Brn3c."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The transgenic mouse of claim 1, wherein expression of a recombinase in a pancreatic beta cell of the mouse results in expression of the fusion protein, and exposure of the mouse to an FKBP dimerizing agent results in an increase in a blood glucose level in the mouse."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The transgenic mouse of claim 14, wherein the mouse expresses a recombinase in a pancreatic beta cell."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The transgenic mouse of claim 15, wherein the recombinase is expressed from a cell-specific promoter."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The transgenic mouse of claim 15, wherein the recombinase is cyclization recombination (Cre) recombinase or flippase (Flp) recombinase."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The transgenic non-human mouse of claim 16, wherein the cell-specific promoter is the insulin promoter."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 7, wherein the recombinase is cyclization recombination (Cre) recombinase or flippase (Flp) recombinase."],"number":19,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}