{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"080-866-916-965-670","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"080-866-916-965-670"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10784,"type":"PATENT","title":"Purdue University - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":7369,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8254,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: \"Purdue Uni*\";
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 467
Search Applicants and Owners separately: \"Purdue Uni*\";
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 467
(a) curating kinase specific input data sets consisting of validated endogenous sequences for peptide substrates and the target kinase family interacting partner sequences;\n
(b) encoding the input data sets from step (a) into positional scoring matrices;\n
(c) manipulating the positional scoring matrices generated in step (b) to identify positions required for kinase selectivity, scoring amino acids overrepresented 4 residues upstream and downstream of a phosphorylated residue;\n
(d) generating a candidate list of peptide sequences based on combinatorial peptide sequences of a result in step (c);\n
(e) comparing the combinatorial peptide sequences of step (d) to the combinatorial peptide sequences for at least one other kinase;\n
(f) using the results of steps (c)-(e), substitute residues at non-selective positions in selections from the candidate list of peptide sequences from step (e) with terbium binding residues by comparing sequence similarity scores to a known terbium binding motif; and\n
(g) screening the selections from the candidate list of peptide sequences with substituted residues from step (f) in vitro for the ability to bind terbium in a phosphorylation dependent manner."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the peptide substrate is a substrate for a tyrosine kinase."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the peptide substrate is a substrate for a serine/threonine kinase."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2, wherein the tyrosine kinase is selected from the group consisting of Abl, Arg, Btk, Csk, Fyn, Hck, Jak2, Lck, Lyn, Pyk2, Src, Syk, and Yes."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4, wherein the tyrosine kinase is Abl."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4, wherein the tyrosine kinase is Arg."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4, wherein the tyrosine kinase is Btk."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4, wherein the tyrosine kinase is Csk."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4, wherein the tyrosine kinase is Fyn."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4, wherein the tyrosine kinase is Jak2."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4, wherein the tyrosine kinase is Lck."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4, wherein the tyrosine kinase is Lyn."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4, wherein the tyrosine kinase is Pyk2."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4, wherein the tyrosine kinase is Src."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4, wherein the tyrosine kinase is Syk."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4, wherein the tyrosine kinase is Hck."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4, wherein the tyrosine kinase is Yes."],"number":17,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}