{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"073-538-371-595-246","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"073-538-371-595-246","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8878,"type":"PATENT","title":"Kyoto University Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":10470,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8212,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately = \"Kyoto University\", \"Kyoto Univ\", \" Univ Kyoto\", \" University Kyoto\", \"Kyot* Univ*\", \"Univ* Kyot*\".
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Search Applicants and Owners separately = \"Kyoto University\", \"Kyoto Univ\", \" Univ Kyoto\", \" University Kyoto\", \"Kyot* Univ*\", \"Univ* Kyot*\".
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(1) culturing the pluripotent stem cell in a medium containing one or more WNT signaling activators, and\n
(2) replacing the medium from step (1) with a medium containing one or more WNT signaling inhibitors wherein a plurality of cardiac differentiated cells are produced,\n
wherein said one or more WNT signaling inhibitors comprise the compound of Formula (I):\n\n
wherein\n\nR1 to R5 are each independently a hydrogen atom; a halogen atom; a hydroxyl group; a linear or branched alkoxy group having 1 to 5 carbon atoms; a linear or branched alkyl group having 1 to 5 carbon atoms which is unsubstituted or substituted with a halogen atom; or a group —NR12R13, wherein R12 and R13 are each independently a hydrogen atom, an oxygen atom, or a linear or branched alkyl group having 1 to 5 carbon atoms which is unsubstituted or substituted with a halogen atom; wherein two adjacent groups among R1 to R5 may join together to form —O—CH2—O—or —O—(CH2)2—O—,\nR6 to R9 are each independently a hydrogen atom; a halogen atom; a hydroxyl group; a linear or branched alkoxy group having 1 to 5 carbon atoms; a linear or branched alkoxy group having 1 to 5 carbon atoms which is substituted with a group —C(O)A, wherein A is a saturated or unsaturated 5- or 6-membered ring which is unsubstituted or substituted with a linear or branched alkyl group having 1 to 5 carbon atoms and the ring may contain 1 or 2 atoms independently selected from a nitrogen atom, an oxygen atom and a sulfur atom; a linear or branched alkyl group having 1 to 5 carbon atoms which is unsubstituted or substituted with a halogen atom; or a group —NR12R13, wherein R12 and R13 are each independently a hydrogen atom, an oxygen atom, or a linear or branched alkyl group having 1 to 5 carbon atoms which is unsubstituted or substituted with a halogen atom; wherein two adjacent groups among R6 to R9 may join together to form —O—CH2—O—or —O—(CH2)2—O—,\nR10 to R11 are each independently a hydrogen atom; or a linear or branched alkyl group having 1 to 5 carbon atoms,\nX is a sulfur atom, and\nn is an integer of 0 to 6,\n
or a salt thereof."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said compound of Formula (I) is selected from the group consisting of\n\n\nor a salt thereof."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 2, wherein the one or more WNT signaling inhibitors comprise\nor a salt thereof."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the one or more WNT signaling inhibitors further comprise a WNT signaling inhibitor selected from XAV939 and IWP-2."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the one or more WNT signaling activators comprise a GSK3β inhibitor."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 5, wherein the GSK3β inhibitor is selected from BIO and CHIR99021."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the medium in the step (1) and the medium in the step (2) do not comprise serum."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the medium in the step (1) and the medium in the step (2) do not comprise a cytokine."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the medium in the step (1) and the medium in the step (2) comprise albumin."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the medium in the step (1) and the medium in the step (2) do not comprise a protein other than albumin."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the two culturing steps are conducted in the absence of a xenogeneic component."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the pluripotent stem cell is a monkey or human pluripotent stem cell."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the differentiated cells include a cardiomyocyte."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["A kit for promoting cardiac differentiation comprising one or more WNT signaling activators and/or one or more WNT signaling inhibitors, wherein the kit is used for the method of claim 1,\n
wherein said one or more WNT signaling inhibitors comprise the compound of Formula (I):\n\n
wherein\n\nor a salt thereof."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein\nR1 to R5 are each independently a hydrogen atom; a halogen atom; a hydroxyl group; a linear or branched alkoxy group having 1 to 5 carbon atoms; a linear or branched alkyl group having 1 to 5 carbon atoms which is unsubstituted or substituted with a halogen atom; or a group —NR12R13, wherein R12, and R13 are each independently a hydrogen atom, an oxygen atom, or a linear or branched alkyl group having 1 to 5 carbon atoms which is unsubstituted or substituted with a halogen atom; wherein two adjacent groups among R1 to R5 may join together to form —O—CH2—O—or —O—(CH2)2—O—,\nR6 to R9 are each independently a hydrogen atom; a halogen atom; a hydroxyl group; a linear or branched alkoxy group having 1 to 5 carbon atoms; a linear or branched alkoxy group having 1 to 5 carbon atoms which is substituted with a group —C(O)A, wherein A is a saturated or unsaturated 5- or 6-membered ring which is unsubstituted or substituted with a linear or branched alkyl group having 1 to 5 carbon atoms and the ring may contain 1 or 2 atoms independently selected from a nitrogen atom, an oxygen atom and a sulfur atom; a linear or branched alkyl group having 1 to 5 carbon atoms which is unsubstituted or substituted with a halogen atom; or a group —NR12R13, wherein R12, and R13 are each independently a hydrogen atom, an oxygen atom, or a linear or branched alkyl group having 1 to 5 carbon atoms which is unsubstituted or substituted with a halogen atom; wherein two adjacent groups among R6 to R9 may join together to form —O—CH2—O—or —O—(CH2)2—O—,\nR10 to R11 are each independently a hydrogen atom; or a linear or branched alkyl group having 1 to 5 carbon atoms,\nX is a sulfur atom, and\nn is an integer of 0 to 6,\n
R1, R4, R5, R6 and R9 each are a hydrogen atom,\n
R2 and R3 are a methoxy group, an ethoxy group or a propoxy group,\n
R10 and R11 are a hydrogen atom, and\n
n is an integer of 0 to 4."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 15, wherein\n
R7 is a halogen atom, and\n
R8 is a hydrogen atom."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 16, wherein n is 2 or 3."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 17, wherein R2 and R3 are a methoxy group."],"number":18,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}