{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"071-959-258-183-752","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"071-959-258-183-752"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8903,"type":"PATENT","title":"Johns Hopkins Univ Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":17938,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8218,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
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Total patents: 12941
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Total patents: 12941
obtaining a biological sample from the subject; and\n
detecting an altered expression level of a gene product of Milk Fat Globule Protein Epidermal Growth Factor-8 (MFG-E8) or at least one other gene listed in Table 1 in the biological sample, relative to a reference level,\nthereby diagnosing or prognosing an age-associated vascular disorder in the subject."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the reference level is the level of expression of the gene product:\n
a) in a biological sample obtained from the same subject at an earlier time point;\n
b) in a biological sample obtained from another subject that does not have the age-associated vascular disorder;\n
c) in a biological sample obtained from a younger individual; or\n
d) a statistical reference value indicative of expression in a subject that does not have the age-associated vascular disorder."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the gene product of MFG-E8 or the at least one other gene listed in Table 1 is a protein and detection of altered expression of the gene product comprises detecting the MFG-E8 protein or other protein listed in Table 1."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, further comprising enrichment of the MFG-E8 protein or other protein listed in Table 1 in the biological sample."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, further comprising:\n
producing a protein digest from the biological sample;\n
and detecting a fragment of the MFG-E8 protein or other protein listed in Table 1 in the protein digest, thereby detecting the MFG-E8 protein or other protein in Table 1 in the biological sample."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 5, wherein the peptide fragment of MFG-E8 comprises the amino acid sequence of any one of SEQ ID NOs: 1, 2 and 9-12."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 6, wherein the peptide fragment of MFG-E8 consists of the amino acid sequence of any one of SEQ ID NOs: 1, 2 and 9-12."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 5, further comprising fractionating the protein digest prior to detecting the fragment of the MFG-E8 protein or other protein listed in Table 1 in the protein digest."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 8, wherein fractionating the protein digest comprises separation of the digest by chromatography."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 9, wherein the chromatography comprises one or more of gel electrophoresis, capillary electrophoresis, nano-reversed phase liquid chromatography, high performance liquid chromatography, reverse phase high performance liquid chromatography, ion exchange chromatography, phosphorylation enrichment affinity chromatography, glycosylation enrichment affinity chromatography, and protein affinity chromatography."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 5, wherein the protein digest comprises a serine protease digest."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 11 wherein the serine protease digest comprises a trypsin digest."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, wherein detecting protein comprises mass spectrometry."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, wherein detecting the MFG-E8 protein or other protein listed in Table 1 comprises contacting the MFG-E8 protein or other protein listed in Table 1 with an antibody that specifically binds the MFG-E8 protein or other protein listed in Table 1."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, further comprising quantitating the MFG-E8 protein or other protein listed in Table 1, or a fragment thereof."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 15, wherein quantitating the MFG-E8 protein or other protein listed in Table 1 comprises comparing an amount of a fragment of the MFG-E8 or other protein listed in Table 1 to a peptide standard of known amount."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 16, wherein the peptide standard comprises a peptide having the amino acid sequence of any one of SEQ ID NOs: 1, 2 or 9-12."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 17, wherein the peptide standard consists of a peptide having the amino acid sequence of any one of SEQ ID NOs: 1, 2 or 9-12."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 16, wherein the peptide standard is an isotopically labeled peptide."],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 19, wherein the isotopically labeled peptide comprises one or more heavy stable isotopes selected from 18O, 17O, 34S, 15N, 13C, 2H or combinations thereof."],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, wherein the MFG-E8 protein or other protein listed in Table 1, or a fragment thereof is post-translationally modified."],"number":21,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the gene product of MFG-E8 or another gene listed in Table 1 is a nucleic acid and detecting altered expression of the gene product comprises detecting the nucleic acid."],"number":22,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 22, wherein detecting the nucleic acid comprises detecting mRNA."],"number":23,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 23, wherein detecting mRNA comprises using a reverse-transcription-polymerase chain reaction (RT-PCR) or mass spectrometry."],"number":24,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 24, wherein the RT-PCR comprises quantitative RT-PCR."],"number":25,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 22, wherein detecting expression comprises using a microarray."],"number":26,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein detecting altered expression further comprises detecting an expression level of MFG-E8 and at least one other gene listed in Table 1."],"number":27,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the altered expression level of MFG-E8 or the other gene listed in Table 1 comprises an increase in the expression level of MFG-E8 or the other gene listed in Table 1."],"number":28,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 28, wherein the increased expression level of MFG-E8 or the other gene listed in Table 1 comprises an increase in protein or nucleic acid level."],"number":29,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the biological sample comprises a blood sample, a urine sample, a serum sample, an ascites sample, a saliva sample, a cell, or a portion of a tissue sample."],"number":30,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 30, wherein the biological sample comprises a serum or a plasma sample."],"number":31,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 31, wherein the tissue sample is obtained by biopsy."],"number":32,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 32, wherein the tissue sample comprises vascular tissue."],"number":33,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}