{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"070-963-946-042-430","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"070-963-946-042-430","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8876,"type":"PATENT","title":"Northwestern University Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":17491,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8210,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search applicants and Owners separately = \" Northwestern University\", 'Northw* Univ*\" , \"Univ Northwestern\", \"
Search applicants and Owners separately = \" Northwestern University\", 'Northw* Univ*\" , \"Univ Northwestern\", \"
(a) gently agitating the stool sample in a wash solution;\n
(c) centrifuging the stool sample in the wash solution to form a cell pellet;\n
(d) disrupting the cell pellet in a cell preservation solution to form a resuspended cell pellet;\n
(e) filtering the resuspended cell pellet through a first strainer and collecting supernatant;\n
(f) filtering the supernatant through a second strainer to retain mucus layer colonocytes; and\n
(g) combining the mucus layer colonocytes with at least one mucolytic agent and at least one chelating agent to form a suspension of isolated mucus layer colonocytes."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the wash solution contains ammonium thioglycolate."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the first strainer is a filter of about 300 μM."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the second strainer is a filter of about 125 μM."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the at least one mucolytic agent is N-acetyl L-cysteine (NALC)."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 5 wherein the NALC comprises about 0.5 mol/L NALC."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 5 wherein the EDTA comprises about 0.0015 mol/L EDTA."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the at least one chelating agent is ethylene diaminetetraacetic acid (EDTA)."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 further comprising applying the suspension of isolated mucus layer colonocytes to a slide."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9 wherein the mucus layer colonocytes are applied to the slide using an automated slide preparation unit."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9 wherein the mucus layer colonocytes are preserved with ethanol."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 further comprising analyzing RNA expression in the mucus layer colonocytes."],"number":12,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}