{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"068-654-804-760-022","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"068-654-804-760-022","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":12063,"type":"PATENT","title":"Wayne State University Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":2773,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8588,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately:Wayne State Univ*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2610
Search Applicants and Owners separately:Wayne State Univ*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2610
allowing the matrix/analyte association to enter the gas phase of an ion source of a mass spectrometer wherein the gas phase comprises an intermediate pressure zone or a high vacuum zone such that the matrix/analyte association is exposed to a decrease in pressure\n
thereby producing the multiply-charged analyte ions."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["A method of claim 1 wherein the gas phase comprises an intermediate pressure zone with a pressure from about 10−3 Torr to about 200 Torr."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["A method of claim 1 wherein the gas phase comprises a high vacuum zone with a pressure from about 10−9 Torr to about 10−3 Torr."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["A method of claim 1 wherein the method further comprises heating an ionization region comprising a sample stage on which the matrix/analyte association is contacted with the force."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["A method of claim 1 wherein the force is a laser beam."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["A method of claim 5 wherein the laser beam contacts the matrix/analyte association through transmission geometry or through reflection geometry."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["A method of claim 1 wherein the matrix is an organic composition that comprises a compound of FIGS. 135A-135L."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["A method of claim 1 wherein the analyte is an intact, enzymatically digested, oxidized, acetylated, methylated, sulfonated, or phosphorylated protein or peptide."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["A method of claim 8 wherein the oxidation, acetylation or phosphorylation of the protein or peptide is indicative of a disease state."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["A method of claim 1 wherein the analyte is a lipid, a lipid including fragile gangliosides, a carbohydrate, an oligonucleotide, a synthetic polymer, a biofilm, a cell culture, a synthetic surface, animal tissue, plant tissue, a drug, a drug metabolite, or an endogenous metabolite."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["A method of claim 1 wherein the mass spectrometer is a high vacuum time-of-flight (TOF) and/or TOF/TOF mass spectrometer and the method extends the fragmentation and mass accuracy associated with multiply charged ions on the mass spectrometer."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["A method of claim 1 wherein the mass spectrometer is a Fourier Transform-Ion Cyclotron Resonance (FT-ICR) mass spectrometer, a Q-TOF mass spectrometer (SYNAPT G2, Waters Corporation, Milford, Mass.) or an Orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, Mass.) and the method extends the mass range, mass accuracy, and fragmentation of the mass spectrometer."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["A method of claim 1 wherein the mass spectrometer is an ion trap, triple quadrupole, and single quadrupole mass spectrometer and the method extends the mass range, mass accuracy, and fragmentation of the mass spectrometer."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["A method of gas phase separation of an analyte comprising\n
contacting a matrix/analyte association with a force that generates transfer and receipt of charge between the matrix and analyte;\n
allowing the matrix/analyte association to enter the gas phase of an ion source of a mass spectrometer wherein the gas phase comprises an intermediate pressure zone or a high vacuum zone such that the matrix/analyte association is exposed to a decrease in pressure\n
thereby producing multiply-charged ions and enabling the differentiation of isomers by cross section shape, and permitting cross section analysis using modeling of ions from surfaces using ion mobility spectrometry-mass spectrometry instrumentation."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["A method of claim 14 wherein the ion mobility spectrometry-mass spectrometry instrumentation is a drift tube, a traveling wave or field asymmetry (FAIMS) technology."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["A method of claim 14 comprising enhancing fragmentation in MS/MS instrumentation through collision induced dissociation, electron transfer dissociation or electron capture dissociation."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["A method of preparing and analyzing a matrix/analyte association using mass spectrometry and/or ion mobility spectrometry-mass spectrometry comprising associating a matrix with an analyte to form a matrix/analyte association before or after deposition onto a metal or glass plate;\n
contacting the matrix/analyte association with a force that dislodges the matrix/analyte association from the plate thereby exposing the matrix/analyte association to an intermediate pressure or high vacuum zone within a mass spectrometer or ion mobility mass spectrometer so that the matrix/analyte association is exposed to a decrease in pressure thereby producing multiply-charged ions that are positively or negatively charged depending on the operation of the mass spectrometer and detector;\n
acquiring a mass spectrum, fragmentation spectrum or IMS data of the analyte; and\n
evaluating the mass spectrum, fragmentation spectrum or IMS data to determine a characteristic of the analyte."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["A method of claim 17 wherein the associating of the matrix with the analyte occurs by mixing an analyte solution with a matrix solution to form a matrix/analyte solution in a separate container or on the sample plate and drying the analyte/matrix solution for form the association or bringing a solid analyte including native surfaces into contact with a matrix in which the matrix can be placed below or on top of the analyte surface."],"number":18,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}